Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022771107> ?p ?o ?g. }
- W2022771107 endingPage "e1002076" @default.
- W2022771107 startingPage "e1002076" @default.
- W2022771107 abstract "Clostridium difficile is the etiological agent of antibiotic-associated diarrhoea (AAD) and pseudomembranous colitis in humans. The role of the surface layer proteins (SLPs) in this disease has not yet been fully explored. The aim of this study was to investigate a role for SLPs in the recognition of C. difficile and the subsequent activation of the immune system. Bone marrow derived dendritic cells (DCs) exposed to SLPs were assessed for production of inflammatory cytokines, expression of cell surface markers and their ability to generate T helper (Th) cell responses. DCs isolated from C3H/HeN and C3H/HeJ mice were used in order to examine whether SLPs are recognised by TLR4. The role of TLR4 in infection was examined in TLR4-deficient mice. SLPs induced maturation of DCs characterised by production of IL-12, TNFα and IL-10 and expression of MHC class II, CD40, CD80 and CD86. Furthermore, SLP-activated DCs generated Th cells producing IFNγ and IL-17. SLPs were unable to activate DCs isolated from TLR4-mutant C3H/HeJ mice and failed to induce a subsequent Th cell response. TLR4−/− and Myd88−/−, but not TRIF−/− mice were more susceptible than wild-type mice to C. difficile infection. Furthermore, SLPs activated NFκB, but not IRF3, downstream of TLR4. Our results indicate that SLPs isolated from C. difficile can activate innate and adaptive immunity and that these effects are mediated by TLR4, with TLR4 having a functional role in experimental C. difficile infection. This suggests an important role for SLPs in the recognition of C. difficile by the immune system." @default.
- W2022771107 created "2016-06-24" @default.
- W2022771107 creator A5004433955 @default.
- W2022771107 creator A5006474195 @default.
- W2022771107 creator A5008400282 @default.
- W2022771107 creator A5021348424 @default.
- W2022771107 creator A5022430976 @default.
- W2022771107 creator A5024299625 @default.
- W2022771107 creator A5027879562 @default.
- W2022771107 creator A5034064298 @default.
- W2022771107 creator A5041413253 @default.
- W2022771107 creator A5052201193 @default.
- W2022771107 creator A5061511792 @default.
- W2022771107 creator A5062328111 @default.
- W2022771107 creator A5064155320 @default.
- W2022771107 creator A5075187737 @default.
- W2022771107 creator A5076098159 @default.
- W2022771107 creator A5078082158 @default.
- W2022771107 creator A5080205405 @default.
- W2022771107 creator A5086513049 @default.
- W2022771107 creator A5090282281 @default.
- W2022771107 creator A5060584763 @default.
- W2022771107 date "2011-06-30" @default.
- W2022771107 modified "2023-10-18" @default.
- W2022771107 title "A Role for TLR4 in Clostridium difficile Infection and the Recognition of Surface Layer Proteins" @default.
- W2022771107 cites W1484335948 @default.
- W2022771107 cites W1524159069 @default.
- W2022771107 cites W1527612695 @default.
- W2022771107 cites W1533878687 @default.
- W2022771107 cites W1702969026 @default.
- W2022771107 cites W1891734594 @default.
- W2022771107 cites W1940704816 @default.
- W2022771107 cites W1961120709 @default.
- W2022771107 cites W1965301424 @default.
- W2022771107 cites W1966505731 @default.
- W2022771107 cites W1970100427 @default.
- W2022771107 cites W1973650764 @default.
- W2022771107 cites W1978926805 @default.
- W2022771107 cites W1980604167 @default.
- W2022771107 cites W1984687595 @default.
- W2022771107 cites W2004465286 @default.
- W2022771107 cites W2007377453 @default.
- W2022771107 cites W2018248632 @default.
- W2022771107 cites W2019676172 @default.
- W2022771107 cites W2025024815 @default.
- W2022771107 cites W2027566765 @default.
- W2022771107 cites W2038267036 @default.
- W2022771107 cites W2056041704 @default.
- W2022771107 cites W2060785255 @default.
- W2022771107 cites W2070597700 @default.
- W2022771107 cites W2073653589 @default.
- W2022771107 cites W2074694828 @default.
- W2022771107 cites W2075515711 @default.
- W2022771107 cites W2083089709 @default.
- W2022771107 cites W2097809605 @default.
- W2022771107 cites W2098672681 @default.
- W2022771107 cites W2101674097 @default.
- W2022771107 cites W2105118935 @default.
- W2022771107 cites W2108975442 @default.
- W2022771107 cites W2109757350 @default.
- W2022771107 cites W2111428445 @default.
- W2022771107 cites W2121576483 @default.
- W2022771107 cites W2122746019 @default.
- W2022771107 cites W2125181303 @default.
- W2022771107 cites W2125573161 @default.
- W2022771107 cites W2128897591 @default.
- W2022771107 cites W2130421043 @default.
- W2022771107 cites W2134715322 @default.
- W2022771107 cites W2135358916 @default.
- W2022771107 cites W2145708021 @default.
- W2022771107 cites W2147036501 @default.
- W2022771107 cites W2147218692 @default.
- W2022771107 cites W2156451877 @default.
- W2022771107 cites W2158896201 @default.
- W2022771107 doi "https://doi.org/10.1371/journal.ppat.1002076" @default.
- W2022771107 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3128122" @default.
- W2022771107 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21738466" @default.
- W2022771107 hasPublicationYear "2011" @default.
- W2022771107 type Work @default.
- W2022771107 sameAs 2022771107 @default.
- W2022771107 citedByCount "139" @default.
- W2022771107 countsByYear W20227711072012 @default.
- W2022771107 countsByYear W20227711072013 @default.
- W2022771107 countsByYear W20227711072014 @default.
- W2022771107 countsByYear W20227711072015 @default.
- W2022771107 countsByYear W20227711072016 @default.
- W2022771107 countsByYear W20227711072017 @default.
- W2022771107 countsByYear W20227711072018 @default.
- W2022771107 countsByYear W20227711072019 @default.
- W2022771107 countsByYear W20227711072020 @default.
- W2022771107 countsByYear W20227711072021 @default.
- W2022771107 countsByYear W20227711072022 @default.
- W2022771107 countsByYear W20227711072023 @default.
- W2022771107 crossrefType "journal-article" @default.
- W2022771107 hasAuthorship W2022771107A5004433955 @default.
- W2022771107 hasAuthorship W2022771107A5006474195 @default.
- W2022771107 hasAuthorship W2022771107A5008400282 @default.
- W2022771107 hasAuthorship W2022771107A5021348424 @default.