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• W2022773372 abstract "Iron oxide nanoparticles have been shown to magnetically label cells in order to visualize them in vivo via MR imaging. This technology has yet to be implemented in insulin secreting cells, thus it is not known whether the presence of these nanoparticles in the cytoplasm of the cells affects insulin secretion. This study investigates the effectiveness and consequence of labeling mouse insulinoma βTC3 and βTC-tet cells with monocrystalline iron oxide nanoparticles (MION). Our data show that MION can be internalized in both βTC3 and βTC-tet cells following a 24 h exposure to 0.02 mg/ml MION solution. The metabolic and secretory activities of both MION-labeled cell lines were statistically indistinguishable from sham treatment. Furthermore, cell viability and apoptosis remained constant throughout the cell’s exposure to MION. Finally, MR images demonstrated significant contrast between labeled and sham-treated cells. Thus, labeling murine insulinoma cell lines with magnetic iron oxide nanoparticles does not hinder their insulin secretion, while it provides MR imaging contrast." @default.
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• W2022773372 date "2004-06-01" @default.
• W2022773372 modified "2023-10-11" @default.
• W2022773372 title "Magnetically labeled insulin-secreting cells" @default.
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• W2022773372 doi "https://doi.org/10.1016/j.bbrc.2004.04.195" @default.
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