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- W2022773810 abstract "The content of α,α′-trehalose in fronds of Selaginella martensii was shown to be 2.4% by isotope dilution analysis. Trehalose-14C supplied to the cut ends of detached fronds did not accumulate, however, but gave a labelling pattern in the leaves identical with that obtained with d-glucose-14C, indicating a common pathway of metabolism, preceded in the case of trehalose by an initial hydrolysis to glucose. A specific trehalase was purified approximately 15-fold from extracts of fronds by precipitation with ammonium sulfate and chromatography on DEAE cellulose. The enzyme had a pH optimum of about 5.7 and a Km for trehalose of 1.7 × 10−3m. Considerable trehalase activity remained bound to particulate debris in the tissue homogenates. A sensitive and rapid assay for trehalase activity is described which depends upon the separation of labeled glucose from trehalose-14C by high-voltage electrophoresis in borate buffer." @default.
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- W2022773810 date "1969-09-01" @default.
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- W2022773810 title "Trehalase activity in Selaginella martensii" @default.
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- W2022773810 doi "https://doi.org/10.1016/0003-9861(69)90469-x" @default.
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