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- W2022774286 abstract "AIMS: To develop a new typing system for group B streptococci based on 35S-methionine-labelled protein profiles of bacterial proteins. METHODS: 377 clinical isolates of group B streptococci were examined by incorporation of 35S-methionine into bacterial proteins under strict anaerobic conditions. After sodium dodecylsulphate-polyacrylamide gel electrophoresis, autoradiography was performed. The patterns produced were visually analysed and categorised into clusters of organisms based on the pattern of band production between 32-46 kilodaltons. RESULTS: 294 of the typed strains classified into seven different groups designated a-g. 32 strains failed to incorporate 35S-methionine sufficiently to be grouped and 11 strains did not fall into one of the seven identified groups. Typability, reproducibility, and discrimination of the system was evident. CONCLUSIONS: This typing system may help to distinguish between colonising and invasive strains of the organism." @default.
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- W2022774286 date "1993-02-01" @default.
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- W2022774286 title "New phenotypic typing scheme for group B streptococci." @default.
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- W2022774286 doi "https://doi.org/10.1136/jcp.46.2.145" @default.
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