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- W2022776676 abstract "A recombinant plasmid containing human interleukin 2 (IL2) cDNA was identified in a cDNA library constructed from mRNA derived from PHA-TPA induced splenocytes. Using this cDNA as a hybridization probe, a DNA fragment containing the IL2 gene was isolated from a collection of hybrid phages derived from human genomic DNA. A unique reading frame was identified from the nucleotide sequence derived from these plasmids coding for a polypeptide of 153 amino acids and containing a putative signal sequence of 20 amino acids. A mature polypeptide starting with either Met-Ala-Pro or Met-Pro was expressed in E.coli under control of the E.colitrap promoter or using a combination of the phage λPL. promoter and a ribosome binding site derived from phage Mu. The bacterial IL2 polypeptide had a molecular weight of 15,000 daltons and accounted for more than 10% of the total E.coli proteins in fully induced cells; it was biologically active in the T-cell specific DNA synthesis assay, even after recovery from a SDS-containing polyacrylamide gel." @default.
- W2022776676 created "2016-06-24" @default.
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- W2022776676 date "1983-01-01" @default.
- W2022776676 modified "2023-10-03" @default.
- W2022776676 title "Molecular cloning of human interleukin 2 cDNA and its expression in<i>E. coli</i>" @default.
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- W2022776676 doi "https://doi.org/10.1093/nar/11.13.4307" @default.
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