Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022779294> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2022779294 endingPage "22" @default.
- W2022779294 startingPage "17" @default.
- W2022779294 abstract "Nociceptin/orphanin FQ (N/OFQ) is the endogenous ligand for the Gi-coupled N/OFQ receptor (NOP). We have examined the effects of chronic exposure of Chinese hamster ovary cells expressing the recombinant human NOP receptor (CHOhNOP) to 1 nM N/OFQ for up to 48 h in the absence and presence of the NOP selective antagonist [Nphe1]N/OFQ (1–13)NH2 ([Nphe1]). Then, either a concentration–response curve for N/OFQ inhibition of cAMP formation was constructed or the cells were homogenized and membrane receptor density was determined using [125I]Y14N/OFQ. There was a time-dependent reduction in pEC50 (without a change in maximum) for N/OFQ with significant differences observed following >24 h of exposure (control pEC50∼9.5; 48 h pretreatment∼8.7). In cells co-exposed to N/OFQ+[Nphe1] for 48 h, there was no reduction in pEC50. There was a compensatory (∼2.5-fold), [Nphe1]-sensitive increase in cAMP mass in cells exposed to N/OFQ for 24–48 h. N/OFQ pretreatment also resulted in a time-dependent [Nphe1]-sensitive loss of cell surface receptors. At 48 h, Bmax was reduced from ∼2.0 to ∼1.3 pmol mg−1 protein without a change in pKd for N/OFQ. There was a positive correlation between pEC50 for cAMP inhibition and Bmax. The lack of effect on maximum cAMP response probably results from receptor overexpression and the creation of a receptor reserve." @default.
- W2022779294 created "2016-06-24" @default.
- W2022779294 creator A5012278399 @default.
- W2022779294 creator A5028341235 @default.
- W2022779294 creator A5037283388 @default.
- W2022779294 creator A5044328039 @default.
- W2022779294 creator A5061719307 @default.
- W2022779294 date "2002-08-01" @default.
- W2022779294 modified "2023-10-18" @default.
- W2022779294 title "Effects of chronic nociceptin/orphanin FQ exposure on cAMP accumulation and receptor density in Chinese hamster ovary cells expressing human nociceptin/orphanin FQ receptors" @default.
- W2022779294 cites W1481824024 @default.
- W2022779294 cites W1988941422 @default.
- W2022779294 cites W1993551761 @default.
- W2022779294 cites W2000324717 @default.
- W2022779294 cites W2014237020 @default.
- W2022779294 cites W2016562510 @default.
- W2022779294 cites W2016697716 @default.
- W2022779294 cites W2032431494 @default.
- W2022779294 cites W2032488957 @default.
- W2022779294 cites W2032580095 @default.
- W2022779294 cites W2034758911 @default.
- W2022779294 cites W2036685400 @default.
- W2022779294 cites W2039648325 @default.
- W2022779294 cites W2049575401 @default.
- W2022779294 cites W2056529600 @default.
- W2022779294 cites W2066624199 @default.
- W2022779294 cites W2069137844 @default.
- W2022779294 cites W2077581224 @default.
- W2022779294 cites W2086804253 @default.
- W2022779294 cites W2099646741 @default.
- W2022779294 cites W2127433644 @default.
- W2022779294 cites W2326647992 @default.
- W2022779294 doi "https://doi.org/10.1016/s0014-2999(02)01606-0" @default.
- W2022779294 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12163101" @default.
- W2022779294 hasPublicationYear "2002" @default.
- W2022779294 type Work @default.
- W2022779294 sameAs 2022779294 @default.
- W2022779294 citedByCount "9" @default.
- W2022779294 countsByYear W20227792942012 @default.
- W2022779294 countsByYear W20227792942013 @default.
- W2022779294 countsByYear W20227792942015 @default.
- W2022779294 countsByYear W20227792942016 @default.
- W2022779294 countsByYear W20227792942018 @default.
- W2022779294 crossrefType "journal-article" @default.
- W2022779294 hasAuthorship W2022779294A5012278399 @default.
- W2022779294 hasAuthorship W2022779294A5028341235 @default.
- W2022779294 hasAuthorship W2022779294A5037283388 @default.
- W2022779294 hasAuthorship W2022779294A5044328039 @default.
- W2022779294 hasAuthorship W2022779294A5061719307 @default.
- W2022779294 hasConcept C125439840 @default.
- W2022779294 hasConcept C126322002 @default.
- W2022779294 hasConcept C134018914 @default.
- W2022779294 hasConcept C159023312 @default.
- W2022779294 hasConcept C170493617 @default.
- W2022779294 hasConcept C175656101 @default.
- W2022779294 hasConcept C185592680 @default.
- W2022779294 hasConcept C2781063702 @default.
- W2022779294 hasConcept C71924100 @default.
- W2022779294 hasConcept C86803240 @default.
- W2022779294 hasConcept C88016717 @default.
- W2022779294 hasConceptScore W2022779294C125439840 @default.
- W2022779294 hasConceptScore W2022779294C126322002 @default.
- W2022779294 hasConceptScore W2022779294C134018914 @default.
- W2022779294 hasConceptScore W2022779294C159023312 @default.
- W2022779294 hasConceptScore W2022779294C170493617 @default.
- W2022779294 hasConceptScore W2022779294C175656101 @default.
- W2022779294 hasConceptScore W2022779294C185592680 @default.
- W2022779294 hasConceptScore W2022779294C2781063702 @default.
- W2022779294 hasConceptScore W2022779294C71924100 @default.
- W2022779294 hasConceptScore W2022779294C86803240 @default.
- W2022779294 hasConceptScore W2022779294C88016717 @default.
- W2022779294 hasIssue "1-2" @default.
- W2022779294 hasLocation W20227792941 @default.
- W2022779294 hasLocation W20227792942 @default.
- W2022779294 hasOpenAccess W2022779294 @default.
- W2022779294 hasPrimaryLocation W20227792941 @default.
- W2022779294 hasRelatedWork W1964433476 @default.
- W2022779294 hasRelatedWork W1988656905 @default.
- W2022779294 hasRelatedWork W2000027332 @default.
- W2022779294 hasRelatedWork W2022779294 @default.
- W2022779294 hasRelatedWork W2039636436 @default.
- W2022779294 hasRelatedWork W2071934673 @default.
- W2022779294 hasRelatedWork W2072073543 @default.
- W2022779294 hasRelatedWork W2889595272 @default.
- W2022779294 hasRelatedWork W2889721340 @default.
- W2022779294 hasRelatedWork W2970224135 @default.
- W2022779294 hasVolume "449" @default.
- W2022779294 isParatext "false" @default.
- W2022779294 isRetracted "false" @default.
- W2022779294 magId "2022779294" @default.
- W2022779294 workType "article" @default.