FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022785608> ?p ?o ?g. }

• W2022785608 endingPage "444" @default.
• W2022785608 startingPage "433" @default.
• W2022785608 abstract "Cystic fibrosis results from mutations in the ABC transporter CFTR (cystic fibrosis transmembrane conductance regulator), which functions as a cAMP-regulated anion channel. The most prevalent mutation in CFTR, the Phe(508) deletion, results in the generation of a trafficking and functionally deficient channel. The cellular machineries involved in modulating CFTR trafficking and function have not been fully characterized. In the present study, we identified a role for the COPI (coatomer protein I) cellular trafficking machinery in the development of the CFTR polypeptide into a functional chloride channel. To examine the role of COPI in CFTR biosynthesis, we employed the cell line ldlF, which harbours a temperature-sensitive mutation in epsilon-COP, a COPI subunit, to inhibit COPI function and then determined whether the CFTR polypeptide produced from the transfected gene developed into a cAMP-regulated chloride channel.When COPI was inactivated in the ldlF cells by an elevated temperature pulse (39 degrees C), the CFTR polypeptide was detected on the cell surface by immunofluorescence microscopy and cell-surface biotinylation. Therefore, CFTR proceeded upstream in the secretory pathway in the absence of COPI function, a result demonstrated previously by others. In contrast, electrophysiological measurements indicated an absence of cAMP-stimulated chloride efflux, suggesting that channel function was impaired. In comparison, expression of CFTR at the same elevated temperature (39 degrees C) in an epsilon-COP-rescued cell line [ldlF(ldlF)], which has an introduced wild-type epsilon-COP gene in addition to the mutant epsilon-COP gene, showed restoration of cAMP-stimulated channel activity, confirming the requirement of COPI for channel function.These results therefore suggest that generation of the folded-functional conformation of CFTR requires COPI." @default.
• W2022785608 created "2016-06-24" @default.
• W2022785608 creator A5003629788 @default.
• W2022785608 creator A5047059421 @default.
• W2022785608 creator A5048409002 @default.
• W2022785608 creator A5053921240 @default.
• W2022785608 creator A5056247736 @default.
• W2022785608 creator A5085382062 @default.
• W2022785608 date "2007-08-01" @default.
• W2022785608 modified "2023-09-24" @default.
• W2022785608 title "Cystic fibrosis transmembrane conductance regulator (CFTR) functionality is dependent on coatomer protein I (COPI)" @default.
• W2022785608 cites W1520171546 @default.
• W2022785608 cites W1551219357 @default.
• W2022785608 cites W1567136101 @default.
• W2022785608 cites W1567198911 @default.
• W2022785608 cites W1745204207 @default.
• W2022785608 cites W1966010415 @default.
• W2022785608 cites W1969273529 @default.
• W2022785608 cites W1971954433 @default.
• W2022785608 cites W1976507289 @default.
• W2022785608 cites W1980770642 @default.
• W2022785608 cites W1985017562 @default.
• W2022785608 cites W1996795653 @default.
• W2022785608 cites W2002426370 @default.
• W2022785608 cites W2009198934 @default.
• W2022785608 cites W2012602248 @default.
• W2022785608 cites W2024714939 @default.
• W2022785608 cites W2032588813 @default.
• W2022785608 cites W2034934027 @default.
• W2022785608 cites W2036420384 @default.
• W2022785608 cites W2037250030 @default.
• W2022785608 cites W2040826404 @default.
• W2022785608 cites W2042062413 @default.
• W2022785608 cites W2050662826 @default.
• W2022785608 cites W2051246945 @default.
• W2022785608 cites W2062238440 @default.
• W2022785608 cites W2065199253 @default.
• W2022785608 cites W2069098570 @default.
• W2022785608 cites W2069512007 @default.
• W2022785608 cites W2071673061 @default.
• W2022785608 cites W2075257287 @default.
• W2022785608 cites W2077416083 @default.
• W2022785608 cites W2080423498 @default.
• W2022785608 cites W2082153375 @default.
• W2022785608 cites W2083422797 @default.
• W2022785608 cites W2087645353 @default.
• W2022785608 cites W2089035269 @default.
• W2022785608 cites W2090425242 @default.
• W2022785608 cites W2102948276 @default.
• W2022785608 cites W2104034787 @default.
• W2022785608 cites W2104360518 @default.
• W2022785608 cites W2109562416 @default.
• W2022785608 cites W2109936231 @default.
• W2022785608 cites W2113418681 @default.
• W2022785608 cites W2116511323 @default.
• W2022785608 cites W2118445531 @default.
• W2022785608 cites W2121563770 @default.
• W2022785608 cites W2124827174 @default.
• W2022785608 cites W2125123967 @default.
• W2022785608 cites W2129208664 @default.
• W2022785608 cites W2134568320 @default.
• W2022785608 cites W2137704498 @default.
• W2022785608 cites W2138405580 @default.
• W2022785608 cites W2141857608 @default.
• W2022785608 cites W2146335990 @default.
• W2022785608 cites W2149142984 @default.
• W2022785608 cites W2150321884 @default.
• W2022785608 cites W2152905229 @default.
• W2022785608 cites W2153928404 @default.
• W2022785608 cites W2160513335 @default.
• W2022785608 cites W2269547969 @default.
• W2022785608 cites W2271705513 @default.
• W2022785608 doi "https://doi.org/10.1042/bc20060114" @default.
• W2022785608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17388782" @default.
• W2022785608 hasPublicationYear "2007" @default.
• W2022785608 type Work @default.
• W2022785608 sameAs 2022785608 @default.
• W2022785608 citedByCount "5" @default.
• W2022785608 countsByYear W20227856082012 @default.
• W2022785608 countsByYear W20227856082015 @default.
• W2022785608 countsByYear W20227856082017 @default.
• W2022785608 crossrefType "journal-article" @default.
• W2022785608 hasAuthorship W2022785608A5003629788 @default.
• W2022785608 hasAuthorship W2022785608A5047059421 @default.
• W2022785608 hasAuthorship W2022785608A5048409002 @default.
• W2022785608 hasAuthorship W2022785608A5053921240 @default.
• W2022785608 hasAuthorship W2022785608A5056247736 @default.
• W2022785608 hasAuthorship W2022785608A5085382062 @default.
• W2022785608 hasConcept C104317684 @default.
• W2022785608 hasConcept C119062480 @default.
• W2022785608 hasConcept C143065580 @default.
• W2022785608 hasConcept C145822097 @default.
• W2022785608 hasConcept C153911025 @default.
• W2022785608 hasConcept C158617107 @default.
• W2022785608 hasConcept C206912223 @default.
• W2022785608 hasConcept C2776938444 @default.
• W2022785608 hasConcept C2778428886 @default.
• W2022785608 hasConcept C54009773 @default.

• next