Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022785786> ?p ?o ?g. }
- W2022785786 endingPage "550" @default.
- W2022785786 startingPage "543" @default.
- W2022785786 abstract "Neuroprotectin D1 (NPD1) may serve an endogenous neuroprotective role in brain ischemic injury, yet the underlying mechanism involved is poorly understood. In the present study, we aimed to investigate whether intracerebroventricular (ICV) injection of NPD1 is neuroprotective against transient focal cerebral ischemia. We also sought to verify the neuroprotective mechanisms of NPD1. Rats subjected to 2 h ischemia followed by reperfusion were treated with NPD1 at 2 h after reperfusion. PD98059 was administered 20 min prior to surgery. Western blot analysis was performed to detect the protein levels of calpain-specific aII-spectrin breakdown products of 145 kDa (SBDP145), transient receptor potential canonical (subtype) 6 (TRPC6) and phosphorylation of cAMP/Ca2+-response element binding protein (p-CREB) at 12, 24 and 48 h after reperfusion. The immunoreactivity of p-CREB and TRPC6 was measured by quantum dot‑based immunofluorescence analysis. Infarct volume and neurological scoring were evaluated at 48 h after reperfusion. NPD1, when applied at 2 h after reperfusion, significantly reduced infarct volumes and increased neurological scores at 48 h after reperfusion, accompanied by elevated TRPC6 and p-CREB activity, and decreased SBDP145 activity. When mitogen‑activated protein kinase kinase (MEK) activity was specifically inhibited, the neuroprotective effect of NPD1 was attenuated and correlated with decreased CREB activity. Our results clearly showed that ICV injection of NPD1 at 2 h after reperfusion improves the neurological status of middle cerebral artery occlusion (MCAO) rats through the inhibition of calpain‑mediated TRPC6 proteolysis and the subsequent activation of CREB via the Ras/MEK/ERK pathway." @default.
- W2022785786 created "2016-06-24" @default.
- W2022785786 creator A5036633220 @default.
- W2022785786 creator A5065856990 @default.
- W2022785786 creator A5081341048 @default.
- W2022785786 creator A5090604937 @default.
- W2022785786 date "2013-06-25" @default.
- W2022785786 modified "2023-09-30" @default.
- W2022785786 title "Neuroprotectin D1 attenuates brain damage induced by transient middle cerebral artery occlusion in rats through TRPC6/CREB pathways" @default.
- W2022785786 cites W1527956197 @default.
- W2022785786 cites W1573180347 @default.
- W2022785786 cites W1580653784 @default.
- W2022785786 cites W1969871031 @default.
- W2022785786 cites W1975421593 @default.
- W2022785786 cites W1981057134 @default.
- W2022785786 cites W1992372295 @default.
- W2022785786 cites W1997991609 @default.
- W2022785786 cites W2000056048 @default.
- W2022785786 cites W2011783556 @default.
- W2022785786 cites W2016619600 @default.
- W2022785786 cites W2020279479 @default.
- W2022785786 cites W2022219828 @default.
- W2022785786 cites W2023762813 @default.
- W2022785786 cites W2030603509 @default.
- W2022785786 cites W2031943544 @default.
- W2022785786 cites W2034861130 @default.
- W2022785786 cites W2042082013 @default.
- W2022785786 cites W2053354990 @default.
- W2022785786 cites W2057128909 @default.
- W2022785786 cites W2077999133 @default.
- W2022785786 cites W2079241925 @default.
- W2022785786 cites W2081070804 @default.
- W2022785786 cites W2087426606 @default.
- W2022785786 cites W2091598446 @default.
- W2022785786 cites W2100726346 @default.
- W2022785786 cites W2103371711 @default.
- W2022785786 cites W2144918294 @default.
- W2022785786 cites W2149225235 @default.
- W2022785786 cites W2163720024 @default.
- W2022785786 cites W2169079203 @default.
- W2022785786 cites W2169436026 @default.
- W2022785786 cites W2316145923 @default.
- W2022785786 cites W2792583460 @default.
- W2022785786 doi "https://doi.org/10.3892/mmr.2013.1543" @default.
- W2022785786 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23799606" @default.
- W2022785786 hasPublicationYear "2013" @default.
- W2022785786 type Work @default.
- W2022785786 sameAs 2022785786 @default.
- W2022785786 citedByCount "38" @default.
- W2022785786 countsByYear W20227857862014 @default.
- W2022785786 countsByYear W20227857862015 @default.
- W2022785786 countsByYear W20227857862016 @default.
- W2022785786 countsByYear W20227857862017 @default.
- W2022785786 countsByYear W20227857862018 @default.
- W2022785786 countsByYear W20227857862019 @default.
- W2022785786 countsByYear W20227857862020 @default.
- W2022785786 countsByYear W20227857862021 @default.
- W2022785786 countsByYear W20227857862022 @default.
- W2022785786 countsByYear W20227857862023 @default.
- W2022785786 crossrefType "journal-article" @default.
- W2022785786 hasAuthorship W2022785786A5036633220 @default.
- W2022785786 hasAuthorship W2022785786A5065856990 @default.
- W2022785786 hasAuthorship W2022785786A5081341048 @default.
- W2022785786 hasAuthorship W2022785786A5090604937 @default.
- W2022785786 hasBestOaLocation W20227857861 @default.
- W2022785786 hasConcept C104317684 @default.
- W2022785786 hasConcept C126322002 @default.
- W2022785786 hasConcept C1629964 @default.
- W2022785786 hasConcept C181199279 @default.
- W2022785786 hasConcept C184235292 @default.
- W2022785786 hasConcept C185592680 @default.
- W2022785786 hasConcept C25498285 @default.
- W2022785786 hasConcept C541997718 @default.
- W2022785786 hasConcept C55493867 @default.
- W2022785786 hasConcept C56928146 @default.
- W2022785786 hasConcept C57074206 @default.
- W2022785786 hasConcept C71924100 @default.
- W2022785786 hasConcept C86339819 @default.
- W2022785786 hasConcept C98274493 @default.
- W2022785786 hasConceptScore W2022785786C104317684 @default.
- W2022785786 hasConceptScore W2022785786C126322002 @default.
- W2022785786 hasConceptScore W2022785786C1629964 @default.
- W2022785786 hasConceptScore W2022785786C181199279 @default.
- W2022785786 hasConceptScore W2022785786C184235292 @default.
- W2022785786 hasConceptScore W2022785786C185592680 @default.
- W2022785786 hasConceptScore W2022785786C25498285 @default.
- W2022785786 hasConceptScore W2022785786C541997718 @default.
- W2022785786 hasConceptScore W2022785786C55493867 @default.
- W2022785786 hasConceptScore W2022785786C56928146 @default.
- W2022785786 hasConceptScore W2022785786C57074206 @default.
- W2022785786 hasConceptScore W2022785786C71924100 @default.
- W2022785786 hasConceptScore W2022785786C86339819 @default.
- W2022785786 hasConceptScore W2022785786C98274493 @default.
- W2022785786 hasIssue "2" @default.
- W2022785786 hasLocation W20227857861 @default.
- W2022785786 hasLocation W20227857862 @default.
- W2022785786 hasOpenAccess W2022785786 @default.
- W2022785786 hasPrimaryLocation W20227857861 @default.