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- W2022787507 abstract "The reduction of the P. pastoris-derived hepatitis B surface antigen (HBsAg) has been investigated by size exclusion chromatography performed in a detergent solution containing 0.3% sodium dodecyl sulfate (SDS) and 0.1 M Tris-HCl, pH 7.0. The HBsAg, reduced under different conditions and passed through the TSK G4000 SW column (600x7.5 mm I.D.) at 0.9 ml min(-1), was resolved into two peaks corresponding to the reduced, monomeric, and non-reduced forms, respectively. Under these conditions, the antigen fraction corresponding to the HBsAg dimer can be separated and completely reduced to monomers by repeated reductive treatment with simultaneous lipid removal. The efficiency of reduction was maximal after sample treatment with an equal volume of a solution containing 417 mM dithiothreitol, 4.2% (w/v) SDS and 16% (v/v) 2-mercaptoethanol. In conclusion, complete reduction of recombinant HBsAg to monomer subunits is possible and depends on the efficiency of lipid removal during the reductive treatment." @default.
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- W2022787507 date "1998-08-01" @default.
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- W2022787507 title "Size-exclusion chromatographic study of the reduction of recombinant hepatitis B surface antigen" @default.
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- W2022787507 doi "https://doi.org/10.1016/s0378-4347(98)00181-9" @default.
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