FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022791047> ?p ?o ?g. }

• W2022791047 endingPage "13987" @default.
• W2022791047 startingPage "13974" @default.
• W2022791047 abstract "The binding of selectins to carbohydrate ligands expressed on leukocytes regulates immunity and inflammation. Among the human selectin ligands, the O-linked glycans at the N-terminus of the leukocyte cell-surface molecule P-selectin glycoprotein ligand-1 (PSGL-1, CD162) are important because they bind all selectins (L-, E-, and P-selectin) with high affinity under hydrodynamic shear conditions. Analysis of glycan microheterogeneity at this site is complicated by the presence of 72 additional potential O-linked glycosylation sites on this mucinous protein. To overcome this limitation, truncated forms of PSGL-1, called PSGL-1 peptide probes, were developed. Ultra-high sensitivity mass spectrometry analysis of glycans released from such probes along with glycoproteomic analysis demonstrate the presence of both the sialyl Lewis-X (sLe(X)) and the di-sialylated T-antigen (NeuAcα2,3Galβ1,3(NeuAcα2,6)GalNAc) at the PSGL-1 N-terminus. Overexpression of glycoprotein-specific ST6GalNAc-transferases (ST6GalNAc1, -2, or -4) in human promyelocytic HL-60 cells altered glycan structures and cell adhesion properties. In particular, ST6GalNAc2 overexpression abrogated cell surface HECA-452/CLA expression, reduced the number of rolling leukocytes on P- and L-selectin-bearing substrates by ~85%, and increased median rolling velocity of remaining cells by 80-150%. Cell rolling on E-selectin was unaltered although the number of adherent cells was reduced by 60%. ST6GalNAc2 partially co-localizes in the Golgi with the core-2 β(1,6)GlcNAc-transferase C2GnT-1. Overall, the data describe the glycan microheterogeneity at the PSGL-1 N-terminus. They suggest that a competition between ST6GalNAc2 and C2GnT-1 for the core-1/Galβ1,3GalNAc glycan may regulate leukocyte adhesion under fluid shear." @default.
• W2022791047 created "2016-06-24" @default.
• W2022791047 creator A5007738960 @default.
• W2022791047 creator A5045831434 @default.
• W2022791047 creator A5054244721 @default.
• W2022791047 creator A5063759058 @default.
• W2022791047 creator A5063933340 @default.
• W2022791047 creator A5064154652 @default.
• W2022791047 creator A5080001443 @default.
• W2022791047 date "2013-05-01" @default.
• W2022791047 modified "2023-10-02" @default.
• W2022791047 title "Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1" @default.
• W2022791047 cites W1491753329 @default.
• W2022791047 cites W1511676968 @default.
• W2022791047 cites W1591879384 @default.
• W2022791047 cites W1609782250 @default.
• W2022791047 cites W1971214150 @default.
• W2022791047 cites W1976484683 @default.
• W2022791047 cites W1981659539 @default.
• W2022791047 cites W1982071884 @default.
• W2022791047 cites W1983177609 @default.
• W2022791047 cites W1994018335 @default.
• W2022791047 cites W1996738918 @default.
• W2022791047 cites W1997196395 @default.
• W2022791047 cites W1998847759 @default.
• W2022791047 cites W1999287861 @default.
• W2022791047 cites W2006791905 @default.
• W2022791047 cites W2011391804 @default.
• W2022791047 cites W2013909472 @default.
• W2022791047 cites W2019781203 @default.
• W2022791047 cites W2022684550 @default.
• W2022791047 cites W2023693583 @default.
• W2022791047 cites W2024475581 @default.
• W2022791047 cites W2026465178 @default.
• W2022791047 cites W2027493099 @default.
• W2022791047 cites W2032587462 @default.
• W2022791047 cites W2047916715 @default.
• W2022791047 cites W2058023151 @default.
• W2022791047 cites W2060512529 @default.
• W2022791047 cites W2067580785 @default.
• W2022791047 cites W2069079409 @default.
• W2022791047 cites W2072821104 @default.
• W2022791047 cites W2074790620 @default.
• W2022791047 cites W2076603801 @default.
• W2022791047 cites W2078363059 @default.
• W2022791047 cites W2084541252 @default.
• W2022791047 cites W2087359011 @default.
• W2022791047 cites W2098721587 @default.
• W2022791047 cites W2103300312 @default.
• W2022791047 cites W2106489698 @default.
• W2022791047 cites W2107877700 @default.
• W2022791047 cites W2124733101 @default.
• W2022791047 cites W2127892958 @default.
• W2022791047 cites W2129405766 @default.
• W2022791047 cites W2139692296 @default.
• W2022791047 cites W2139715930 @default.
• W2022791047 cites W2145731544 @default.
• W2022791047 cites W2147696500 @default.
• W2022791047 cites W2164438030 @default.
• W2022791047 cites W49723729 @default.
• W2022791047 doi "https://doi.org/10.1074/jbc.m113.463653" @default.
• W2022791047 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3656257" @default.
• W2022791047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23548905" @default.
• W2022791047 hasPublicationYear "2013" @default.
• W2022791047 type Work @default.
• W2022791047 sameAs 2022791047 @default.
• W2022791047 citedByCount "41" @default.
• W2022791047 countsByYear W20227910472013 @default.
• W2022791047 countsByYear W20227910472014 @default.
• W2022791047 countsByYear W20227910472015 @default.
• W2022791047 countsByYear W20227910472016 @default.
• W2022791047 countsByYear W20227910472017 @default.
• W2022791047 countsByYear W20227910472018 @default.
• W2022791047 countsByYear W20227910472019 @default.
• W2022791047 countsByYear W20227910472020 @default.
• W2022791047 countsByYear W20227910472021 @default.
• W2022791047 countsByYear W20227910472022 @default.
• W2022791047 countsByYear W20227910472023 @default.
• W2022791047 crossrefType "journal-article" @default.
• W2022791047 hasAuthorship W2022791047A5007738960 @default.
• W2022791047 hasAuthorship W2022791047A5045831434 @default.
• W2022791047 hasAuthorship W2022791047A5054244721 @default.
• W2022791047 hasAuthorship W2022791047A5063759058 @default.
• W2022791047 hasAuthorship W2022791047A5063933340 @default.
• W2022791047 hasAuthorship W2022791047A5064154652 @default.
• W2022791047 hasAuthorship W2022791047A5080001443 @default.
• W2022791047 hasBestOaLocation W20227910471 @default.
• W2022791047 hasConcept C108625454 @default.
• W2022791047 hasConcept C116569031 @default.
• W2022791047 hasConcept C1491633281 @default.
• W2022791047 hasConcept C153911025 @default.
• W2022791047 hasConcept C155619193 @default.
• W2022791047 hasConcept C16224149 @default.
• W2022791047 hasConcept C170493617 @default.
• W2022791047 hasConcept C181199279 @default.
• W2022791047 hasConcept C185592680 @default.
• W2022791047 hasConcept C204641814 @default.
• W2022791047 hasConcept C206212055 @default.

• next