FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022792234> ?p ?o ?g. }

• W2022792234 endingPage "942" @default.
• W2022792234 startingPage "932" @default.
• W2022792234 abstract "ABSTRACT The bulk of bacterial protein secretion occurs through the conserved SecY translocation channel that is powered by SecA-dependent ATP hydrolysis. Many Gram-positive bacteria, including the human pathogen Listeria monocytogenes , possess an additional nonessential specialized ATPase, SecA2. SecA2-dependent secretion is required for normal cell morphology and virulence in L. monocytogenes ; however, the mechanism of export via this pathway is poorly understood. L. monocytogenes secA2 mutants form rough colonies, have septation defects, are impaired for swarming motility, and form small plaques in tissue culture cells. In this study, 70 spontaneous mutants were isolated that restored swarming motility to L. monocytogenes secA2 mutants. Most of the mutants had smooth colony morphology and septated normally, but all were lysozyme sensitive. Five representative mutants were subjected to whole-genome sequencing. Four of the five had mutations in proteins encoded by the lmo2769 operon that conferred lysozyme sensitivity and increased swarming but did not rescue virulence defects. A point mutation in secY was identified that conferred smooth colony morphology to secA2 mutants, restored wild-type plaque formation, and increased virulence in mice. This secY mutation resembled a prl suppressor known to expand the repertoire of proteins secreted through the SecY translocation complex. Accordingly, the Δ secA2prlA1 mutant showed wild-type secretion levels of P60, an established SecA2-dependent secreted autolysin. Although the prl mutation largely suppressed almost all of the measurable SecA2-dependent traits, the Δ secA2prlA1 mutant was still less virulent in vivo than the wild-type strain, suggesting that SecA2 function was still required for pathogenesis." @default.
• W2022792234 created "2016-06-24" @default.
• W2022792234 creator A5002707693 @default.
• W2022792234 creator A5012504037 @default.
• W2022792234 creator A5024640246 @default.
• W2022792234 date "2015-03-01" @default.
• W2022792234 modified "2023-09-27" @default.
• W2022792234 title "A <i>prl</i> Mutation in SecY Suppresses Secretion and Virulence Defects of Listeria monocytogenes secA2 Mutants" @default.
• W2022792234 cites W106446473 @default.
• W2022792234 cites W1747708829 @default.
• W2022792234 cites W1850330698 @default.
• W2022792234 cites W1889870303 @default.
• W2022792234 cites W1895557972 @default.
• W2022792234 cites W1899820272 @default.
• W2022792234 cites W1964212831 @default.
• W2022792234 cites W1966347327 @default.
• W2022792234 cites W1981801930 @default.
• W2022792234 cites W1985213836 @default.
• W2022792234 cites W1992800404 @default.
• W2022792234 cites W1993356535 @default.
• W2022792234 cites W1994019126 @default.
• W2022792234 cites W1994226427 @default.
• W2022792234 cites W1995517639 @default.
• W2022792234 cites W2006946062 @default.
• W2022792234 cites W2014047550 @default.
• W2022792234 cites W2017066482 @default.
• W2022792234 cites W2024060791 @default.
• W2022792234 cites W2030360373 @default.
• W2022792234 cites W2031269216 @default.
• W2022792234 cites W2031647212 @default.
• W2022792234 cites W2035295085 @default.
• W2022792234 cites W2035752210 @default.
• W2022792234 cites W2041976154 @default.
• W2022792234 cites W2044246903 @default.
• W2022792234 cites W2046185634 @default.
• W2022792234 cites W2046417457 @default.
• W2022792234 cites W2048528832 @default.
• W2022792234 cites W2053541349 @default.
• W2022792234 cites W2059212804 @default.
• W2022792234 cites W2062523428 @default.
• W2022792234 cites W2066510985 @default.
• W2022792234 cites W2069897942 @default.
• W2022792234 cites W2076198022 @default.
• W2022792234 cites W2088888346 @default.
• W2022792234 cites W2088909375 @default.
• W2022792234 cites W2097534440 @default.
• W2022792234 cites W2099899918 @default.
• W2022792234 cites W2100071391 @default.
• W2022792234 cites W2107174986 @default.
• W2022792234 cites W2107477483 @default.
• W2022792234 cites W2108234281 @default.
• W2022792234 cites W2108742330 @default.
• W2022792234 cites W2110125929 @default.
• W2022792234 cites W2115502948 @default.
• W2022792234 cites W2120047279 @default.
• W2022792234 cites W2124081897 @default.
• W2022792234 cites W2126221140 @default.
• W2022792234 cites W2126520065 @default.
• W2022792234 cites W2126569085 @default.
• W2022792234 cites W2129624971 @default.
• W2022792234 cites W2133884021 @default.
• W2022792234 cites W2136038023 @default.
• W2022792234 cites W2136361611 @default.
• W2022792234 cites W2139855150 @default.
• W2022792234 cites W2140378007 @default.
• W2022792234 cites W2140472867 @default.
• W2022792234 cites W2148537117 @default.
• W2022792234 cites W2155234325 @default.
• W2022792234 cites W2155975415 @default.
• W2022792234 cites W2160672142 @default.
• W2022792234 cites W2162340653 @default.
• W2022792234 cites W2162717091 @default.
• W2022792234 cites W2163610076 @default.
• W2022792234 cites W2167198550 @default.
• W2022792234 cites W2168212223 @default.
• W2022792234 cites W2169721606 @default.
• W2022792234 cites W2170551349 @default.
• W2022792234 cites W2171654260 @default.
• W2022792234 cites W4212861730 @default.
• W2022792234 cites W59314449 @default.
• W2022792234 doi "https://doi.org/10.1128/jb.02284-14" @default.
• W2022792234 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4325107" @default.
• W2022792234 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25535272" @default.
• W2022792234 hasPublicationYear "2015" @default.
• W2022792234 type Work @default.
• W2022792234 sameAs 2022792234 @default.
• W2022792234 citedByCount "21" @default.
• W2022792234 countsByYear W20227922342016 @default.
• W2022792234 countsByYear W20227922342017 @default.
• W2022792234 countsByYear W20227922342019 @default.
• W2022792234 countsByYear W20227922342020 @default.
• W2022792234 countsByYear W20227922342021 @default.
• W2022792234 countsByYear W20227922342022 @default.
• W2022792234 crossrefType "journal-article" @default.
• W2022792234 hasAuthorship W2022792234A5002707693 @default.
• W2022792234 hasAuthorship W2022792234A5012504037 @default.
• W2022792234 hasAuthorship W2022792234A5024640246 @default.
• W2022792234 hasBestOaLocation W20227922341 @default.

• next