FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022792630> ?p ?o ?g. }

• W2022792630 endingPage "386" @default.
• W2022792630 startingPage "361" @default.
• W2022792630 abstract "Group II intron mobility occurs by a target DNA-primed reverse transcription mechanism in which the intron RNA reverse splices directly into one strand of a double-stranded DNA target site, while the intron-encoded protein cleaves the opposite strand and uses it as a primer to reverse transcribe the inserted intron RNA. The group II intron endonuclease, which mediates this process, is an RNP particle that contains the intron-encoded protein and the excised intron RNA and uses both cooperatively to recognize DNA target sequences. Here, we analyzed the interaction of the Lactococcus lactis Ll.LtrB group II intron endonuclease with its DNA target site by DNA footprinting and modification-interference approaches. In agreement with previous mutagenesis experiments showing a relatively large target site, DNase I protection extends from position -25 to +19 from the intron-insertion site on the top strand and from -28 to +16 on the bottom strand. Our results suggest that the protein first recognizes a small number of specific bases in the distal 5'-exon region of the DNA target site via major-groove interactions. These base interactions together with additional phosphodiester-backbone interactions along one face of the helix promote DNA unwinding, enabling the intron RNA to base-pair to DNA top-strand positions -12 to +3 for reverse splicing. Notably, DNA unwinding extends to at least position +6, somewhat beyond the region that base-pairs with the intron RNA, but is not dependent on interaction of the conserved endonuclease domain with the 3' exon. Bottom-strand cleavage occurs after reverse splicing and requires recognition of a small number of additional bases in the 3' exon, the most critical being T+5 in the now single-stranded downstream region of the target site. Our results provide the first detailed view of the interaction of a group II intron endonuclease with its DNA target site." @default.
• W2022792630 created "2016-06-24" @default.
• W2022792630 creator A5004611185 @default.
• W2022792630 creator A5005988075 @default.
• W2022792630 date "2001-06-01" @default.
• W2022792630 modified "2023-09-23" @default.
• W2022792630 title "Interaction of a group II intron ribonucleoprotein endonuclease with its DNA target site investigated by DNA footprinting and modification interference" @default.
• W2022792630 cites W1003162216 @default.
• W2022792630 cites W1259363667 @default.
• W2022792630 cites W1840552006 @default.
• W2022792630 cites W1949630295 @default.
• W2022792630 cites W1965423166 @default.
• W2022792630 cites W1970040635 @default.
• W2022792630 cites W1975260126 @default.
• W2022792630 cites W1982878977 @default.
• W2022792630 cites W1986586522 @default.
• W2022792630 cites W1989250056 @default.
• W2022792630 cites W1992595091 @default.
• W2022792630 cites W1992621299 @default.
• W2022792630 cites W1994715746 @default.
• W2022792630 cites W2006183148 @default.
• W2022792630 cites W2007099197 @default.
• W2022792630 cites W2010750399 @default.
• W2022792630 cites W2011897495 @default.
• W2022792630 cites W2014601660 @default.
• W2022792630 cites W2015831954 @default.
• W2022792630 cites W2017863429 @default.
• W2022792630 cites W2018226449 @default.
• W2022792630 cites W2019534246 @default.
• W2022792630 cites W2020556436 @default.
• W2022792630 cites W2020637859 @default.
• W2022792630 cites W2021960562 @default.
• W2022792630 cites W2034583465 @default.
• W2022792630 cites W2037791873 @default.
• W2022792630 cites W2043810954 @default.
• W2022792630 cites W2053199483 @default.
• W2022792630 cites W2055093811 @default.
• W2022792630 cites W2057153975 @default.
• W2022792630 cites W2068825288 @default.
• W2022792630 cites W2075921959 @default.
• W2022792630 cites W2079781055 @default.
• W2022792630 cites W2090669079 @default.
• W2022792630 cites W2118410076 @default.
• W2022792630 cites W2120202917 @default.
• W2022792630 cites W2131968507 @default.
• W2022792630 cites W2133335149 @default.
• W2022792630 cites W2161717286 @default.
• W2022792630 cites W2221827731 @default.
• W2022792630 cites W2231646766 @default.
• W2022792630 doi "https://doi.org/10.1006/jmbi.2001.4658" @default.
• W2022792630 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11371159" @default.
• W2022792630 hasPublicationYear "2001" @default.
• W2022792630 type Work @default.
• W2022792630 sameAs 2022792630 @default.
• W2022792630 citedByCount "89" @default.
• W2022792630 countsByYear W20227926302012 @default.
• W2022792630 countsByYear W20227926302013 @default.
• W2022792630 countsByYear W20227926302014 @default.
• W2022792630 countsByYear W20227926302015 @default.
• W2022792630 countsByYear W20227926302016 @default.
• W2022792630 countsByYear W20227926302018 @default.
• W2022792630 countsByYear W20227926302019 @default.
• W2022792630 countsByYear W20227926302021 @default.
• W2022792630 countsByYear W20227926302022 @default.
• W2022792630 crossrefType "journal-article" @default.
• W2022792630 hasAuthorship W2022792630A5004611185 @default.
• W2022792630 hasAuthorship W2022792630A5005988075 @default.
• W2022792630 hasConcept C104317684 @default.
• W2022792630 hasConcept C119489563 @default.
• W2022792630 hasConcept C153911025 @default.
• W2022792630 hasConcept C2777028655 @default.
• W2022792630 hasConcept C2778021871 @default.
• W2022792630 hasConcept C3017666073 @default.
• W2022792630 hasConcept C54355233 @default.
• W2022792630 hasConcept C54458228 @default.
• W2022792630 hasConcept C552990157 @default.
• W2022792630 hasConcept C64962909 @default.
• W2022792630 hasConcept C67705224 @default.
• W2022792630 hasConcept C82381507 @default.
• W2022792630 hasConcept C86803240 @default.
• W2022792630 hasConcept C94671646 @default.
• W2022792630 hasConceptScore W2022792630C104317684 @default.
• W2022792630 hasConceptScore W2022792630C119489563 @default.
• W2022792630 hasConceptScore W2022792630C153911025 @default.
• W2022792630 hasConceptScore W2022792630C2777028655 @default.
• W2022792630 hasConceptScore W2022792630C2778021871 @default.
• W2022792630 hasConceptScore W2022792630C3017666073 @default.
• W2022792630 hasConceptScore W2022792630C54355233 @default.
• W2022792630 hasConceptScore W2022792630C54458228 @default.
• W2022792630 hasConceptScore W2022792630C552990157 @default.
• W2022792630 hasConceptScore W2022792630C64962909 @default.
• W2022792630 hasConceptScore W2022792630C67705224 @default.
• W2022792630 hasConceptScore W2022792630C82381507 @default.
• W2022792630 hasConceptScore W2022792630C86803240 @default.
• W2022792630 hasConceptScore W2022792630C94671646 @default.
• W2022792630 hasIssue "2" @default.
• W2022792630 hasLocation W20227926301 @default.
• W2022792630 hasLocation W20227926302 @default.

• next