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- W2022793552 abstract "Post-transcriptional gene silencing is commonly observed in polyploid species and often poses a major limitation to plant improvement via biotechnology. Five plant viral suppressors of RNA silencing were evaluated for their ability to counteract gene silencing and enhance the expression of the Enhanced Yellow Fluorescent Protein (EYFP) or the β-glucuronidase (GUS) reporter gene in sugarcane, a major sugar and biomass producing polyploid. Functionality of these suppressors was first verified in Nicotiana benthamiana and onion epidermal cells, and later tested by transient expression in sugarcane young leaf segments and protoplasts. In young leaf segments co-expressing a suppressor, EYFP reached its maximum expression at 48–96 h post-DNA introduction and maintained its peak expression for a longer time compared with that in the absence of a suppressor. Among the five suppressors, Tomato bushy stunt virus-encoded P19 and Barley stripe mosaic virus-encoded γb were the most efficient. Co-expression with P19 and γb enhanced EYFP expression 4.6-fold and 3.6-fold in young leaf segments, and GUS activity 2.3-fold and 2.4-fold in protoplasts compared with those in the absence of a suppressor, respectively. In transgenic sugarcane, co-expression of GUS and P19 suppressor showed the highest accumulation of GUS levels with an average of 2.7-fold more than when GUS was expressed alone, with no detrimental phenotypic effects. The two established transient expression assays, based on young leaf segments and protoplasts, and confirmed by stable transgene expression, offer a rapid versatile system to verify the efficiency of RNA silencing suppressors that proved to be valuable in enhancing and stabilizing transgene expression in sugarcane." @default.
- W2022793552 created "2016-06-24" @default.
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- W2022793552 date "2013-06-14" @default.
- W2022793552 modified "2023-09-26" @default.
- W2022793552 title "Enhanced Transgene Expression in Sugarcane by Co-Expression of Virus-Encoded RNA Silencing Suppressors" @default.
- W2022793552 cites W144423133 @default.
- W2022793552 cites W1504404542 @default.
- W2022793552 cites W1518586105 @default.
- W2022793552 cites W1539503448 @default.
- W2022793552 cites W1567505127 @default.
- W2022793552 cites W160384607 @default.
- W2022793552 cites W1677297091 @default.
- W2022793552 cites W1925002711 @default.
- W2022793552 cites W1950258105 @default.
- W2022793552 cites W1965148885 @default.
- W2022793552 cites W1975050099 @default.
- W2022793552 cites W1975651397 @default.
- W2022793552 cites W1979418286 @default.
- W2022793552 cites W1979965542 @default.
- W2022793552 cites W1983389451 @default.
- W2022793552 cites W1986322119 @default.
- W2022793552 cites W1990425635 @default.
- W2022793552 cites W1997585530 @default.
- W2022793552 cites W2007959167 @default.
- W2022793552 cites W2009211919 @default.
- W2022793552 cites W2013393496 @default.
- W2022793552 cites W2015456365 @default.
- W2022793552 cites W2017743929 @default.
- W2022793552 cites W2018731279 @default.
- W2022793552 cites W2022605927 @default.
- W2022793552 cites W2024556021 @default.
- W2022793552 cites W2028046624 @default.
- W2022793552 cites W2028454636 @default.
- W2022793552 cites W2030083133 @default.
- W2022793552 cites W2030267802 @default.
- W2022793552 cites W2036828137 @default.
- W2022793552 cites W2039956664 @default.
- W2022793552 cites W2040150052 @default.
- W2022793552 cites W2042335471 @default.
- W2022793552 cites W2043809230 @default.
- W2022793552 cites W2047565250 @default.
- W2022793552 cites W2052821301 @default.
- W2022793552 cites W2052839443 @default.
- W2022793552 cites W2055632313 @default.
- W2022793552 cites W2058967345 @default.
- W2022793552 cites W2059992279 @default.
- W2022793552 cites W2062188301 @default.
- W2022793552 cites W2069065184 @default.
- W2022793552 cites W2072133011 @default.
- W2022793552 cites W2072680951 @default.
- W2022793552 cites W2076043262 @default.
- W2022793552 cites W2076628997 @default.
- W2022793552 cites W2079671380 @default.
- W2022793552 cites W2082997857 @default.
- W2022793552 cites W2083698039 @default.
- W2022793552 cites W2085916168 @default.
- W2022793552 cites W2088037129 @default.
- W2022793552 cites W2088978128 @default.
- W2022793552 cites W2094830599 @default.
- W2022793552 cites W2096512019 @default.
- W2022793552 cites W2100925430 @default.
- W2022793552 cites W2103743117 @default.
- W2022793552 cites W2108733083 @default.
- W2022793552 cites W2111401971 @default.
- W2022793552 cites W2112167962 @default.
- W2022793552 cites W2112234428 @default.
- W2022793552 cites W2114165797 @default.
- W2022793552 cites W2114683332 @default.
- W2022793552 cites W2115246339 @default.
- W2022793552 cites W2122430167 @default.
- W2022793552 cites W2126106778 @default.
- W2022793552 cites W2129401531 @default.
- W2022793552 cites W2136433950 @default.
- W2022793552 cites W2138498005 @default.
- W2022793552 cites W2138835651 @default.
- W2022793552 cites W2153327762 @default.
- W2022793552 cites W2155985666 @default.
- W2022793552 cites W2157212209 @default.
- W2022793552 cites W2159190071 @default.
- W2022793552 cites W2159767268 @default.
- W2022793552 cites W2161617747 @default.
- W2022793552 cites W2163323887 @default.
- W2022793552 cites W2166880408 @default.
- W2022793552 cites W2167878665 @default.
- W2022793552 cites W2411914397 @default.