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• W2022794918 endingPage "e60566" @default.
• W2022794918 startingPage "e60566" @default.
• W2022794918 abstract "Human Immunodeficiency virus type-1 (HIV) entry into target cells involves binding of the viral envelope (Env) to CD4 and a coreceptor, mainly CCR5 or CXCR4. The only currently licensed HIV entry inhibitor, maraviroc, targets CCR5, and the presence of CXCX4-using strains must be excluded prior to treatment. Co-receptor usage can be assessed by phenotypic assays or through genotypic prediction. Here we compared the performance of a phenotypic Env-Recombinant Viral Assay (RVA) to the two most widely used genotypic prediction algorithms, Geno2Pheno[coreceptor] and webPSSM.Co-receptor tropism of samples from 73 subtype B and 219 non-B infections was measured phenotypically using a luciferase-tagged, NL4-3-based, RVA targeting Env. In parallel, tropism was inferred genotypically from the corresponding V3-loop sequences using Geno2Pheno[coreceptor] (5-20% FPR) and webPSSM-R5X4. For discordant samples, phenotypic outcome was retested using co-receptor antagonists or the validated Trofile® Enhanced-Sensitivity-Tropism-Assay.The lower detection limit of the RVA was 2.5% and 5% for X4 and R5 minority variants respectively. A phenotype/genotype result was obtained for 210 samples. Overall, concordance of phenotypic results with Geno2Pheno[coreceptor] was 85.2% and concordance with webPSSM was 79.5%. For subtype B, concordance with Geno2pheno[coreceptor] was 94.4% and concordance with webPSSM was 79.6%. High concordance of genotypic tools with phenotypic outcome was seen for subtype C (90% for both tools). Main discordances involved CRF01_AE and CRF02_AG for both algorithms (CRF01_AE: 35.9% discordances with Geno2Pheno[coreceptor] and 28.2% with webPSSM; CRF02_AG: 20.7% for both algorithms). Genotypic prediction overestimated CXCR4-usage for both CRFs. For webPSSM, 40% discordance was observed for subtype A.Phenotypic assays remain the most accurate for most non-B subtypes and new subtype-specific rules should be developed for non-B subtypes, as research studies more and more draw conclusions from genotypically-inferred tropism, and to avoid unnecessarily precluding patients with limited treatment options from receiving maraviroc or other entry inhibitors." @default.
• W2022794918 created "2016-06-24" @default.
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• W2022794918 date "2013-05-08" @default.
• W2022794918 modified "2023-10-14" @default.
• W2022794918 title "HIV-1 Tropism Determination Using a Phenotypic Env Recombinant Viral Assay Highlights Overestimation of CXCR4-Usage by Genotypic Prediction Algorithms for CRRF01_AE and CRF02_AG" @default.
• W2022794918 cites W1486807506 @default.
• W2022794918 cites W1571027354 @default.
• W2022794918 cites W1623426438 @default.
• W2022794918 cites W1664573657 @default.
• W2022794918 cites W1794010035 @default.
• W2022794918 cites W1965019797 @default.
• W2022794918 cites W1981440980 @default.
• W2022794918 cites W1981644527 @default.
• W2022794918 cites W1981724123 @default.
• W2022794918 cites W1985722970 @default.
• W2022794918 cites W1988215875 @default.
• W2022794918 cites W1989035987 @default.
• W2022794918 cites W1996183144 @default.
• W2022794918 cites W1999012053 @default.
• W2022794918 cites W2012848188 @default.
• W2022794918 cites W2018808081 @default.
• W2022794918 cites W2020774496 @default.
• W2022794918 cites W2024190037 @default.
• W2022794918 cites W2024353108 @default.
• W2022794918 cites W2024977015 @default.
• W2022794918 cites W2035864527 @default.
• W2022794918 cites W2039774912 @default.
• W2022794918 cites W2040186892 @default.
• W2022794918 cites W2043182977 @default.
• W2022794918 cites W2048448752 @default.
• W2022794918 cites W2050839471 @default.
• W2022794918 cites W2062148688 @default.
• W2022794918 cites W2063701397 @default.
• W2022794918 cites W2064541590 @default.
• W2022794918 cites W2065839886 @default.
• W2022794918 cites W2076753005 @default.
• W2022794918 cites W2081321475 @default.
• W2022794918 cites W2088668371 @default.
• W2022794918 cites W2096211018 @default.
• W2022794918 cites W2099308199 @default.
• W2022794918 cites W2099959630 @default.
• W2022794918 cites W2100265029 @default.
• W2022794918 cites W2104275641 @default.
• W2022794918 cites W2104289712 @default.
• W2022794918 cites W2104317084 @default.
• W2022794918 cites W2104844807 @default.
• W2022794918 cites W2105307973 @default.
• W2022794918 cites W2108256128 @default.
• W2022794918 cites W2113282092 @default.
• W2022794918 cites W2113633275 @default.
• W2022794918 cites W2114903401 @default.
• W2022794918 cites W2116721805 @default.
• W2022794918 cites W2124212352 @default.
• W2022794918 cites W2128260028 @default.
• W2022794918 cites W2128618062 @default.
• W2022794918 cites W2129663454 @default.
• W2022794918 cites W2132178596 @default.
• W2022794918 cites W2135643648 @default.
• W2022794918 cites W2141692653 @default.
• W2022794918 cites W2149529960 @default.
• W2022794918 cites W2152026267 @default.
• W2022794918 cites W2152327272 @default.
• W2022794918 cites W2154247239 @default.
• W2022794918 cites W2158474242 @default.
• W2022794918 cites W2159899578 @default.
• W2022794918 cites W2164501148 @default.
• W2022794918 cites W2165236706 @default.
• W2022794918 cites W2166136759 @default.
• W2022794918 cites W2166237809 @default.
• W2022794918 cites W2168530883 @default.
• W2022794918 cites W2169054534 @default.
• W2022794918 cites W2319918582 @default.
• W2022794918 cites W2321170269 @default.
• W2022794918 doi "https://doi.org/10.1371/journal.pone.0060566" @default.
• W2022794918 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3734325" @default.
• W2022794918 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23667426" @default.
• W2022794918 hasPublicationYear "2013" @default.
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