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- W2022796188 abstract "Abstract Recombinant DNA methods were used to begin a molecular biological study of the biotechnically important lipase produced by the fungus Rhlzopus delemar. Pure, high molecular weight DNA was isolated, mildly digested with Mbo I, ligated into pBR322, and introduced into E. coli by transformation. Transformants were recovered by ampicillin selection. The frequency of insertional inactivation of tetracycline resistance in the transformants was 95%. The cloned DNA inserts ranged in size from approximately 0 to 14 kilobases (average: 4.7). Colony hybridization using fungal genomic DNA as a probe verified the presence of fungal sequences in the transformed cells. A rapid, sensitive assay for lipase production was developed and applied to a sufficient number of transformants to represent several genomic equivalents of fungal DNA. No lipase‐producing clones were detected." @default.
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- W2022796188 date "1990-01-01" @default.
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- W2022796188 title "Construction of aRhizopus delemargenomic library and screening for direct lipase gene expression" @default.
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- W2022796188 doi "https://doi.org/10.1080/08905439009549780" @default.
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