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- W2022800903 abstract "N,N-Dimethyl, diethyl, dipropyl, dibutyl, and N-monoisopropylaminonaphthylenesulfonyl derivatives of melanotropin inhibiting factor (MIF) and its metabolites were prepared, and their chromatographic behavior was investigated with thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC), using five solvent systems on polyamide layers and ten solvent systems on μBondapak C18 and μBondapak phenyl columns. A mixture of MIF and its metabolites derivatized with Dns chloride was adequately resolved by two-dimensional chromatography on polyamide layer with solvent systems, formic acid-water (3:97) and benzene-acetic acid (9:1). Bns-MIF and its metabolites were separated with μBondapak C18 column with the solvent system acetonitrile-0.01 M sodium sulphate buffer, pH 7 (50:50). They were separated into five groups: Gly and Bns acid; Pro-Leu, Leu-Gly and Leu; Pro; Gly-NH2; and MIF. The alkylaminonaphthylenesulfonyl derivatives had strong fluorescence, which permitted their detection at the level of 10−11 to 10−9 mol. Dns-MIF and its derivatives had the lowest detectable amounts. HPLC with the aid of the Dns derivatization is reliable and fast, and is the preferable method for study of neuropeptide breakdown." @default.
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- W2022800903 date "1980-05-01" @default.
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- W2022800903 title "Separation of alkylaminonaphthylenesulfonyl peptides and amino acids by high-performance liquid chromatography Methods for measuring melanotropin inhibiting factor breakdown" @default.
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- W2022800903 doi "https://doi.org/10.1016/s0021-9673(80)80009-4" @default.
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