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- W2022802337 abstract "Survival of Escherichia coli O157:H7 and Campylobacter jejuni that were separately inoculated into bottled purified drinking water was investigated during storage at 22, 4, and -18 °C for 5, 7, and 2 days, respectively. Two inoculation levels were used, 1 and 10 CFU/ml (10(2) and 10(3) CFU/100 ml). In samples inoculated with 10(2) CFU/100 ml, C. jejuni was not detectable (>2-log reduction) after storage under the conditions specified above. E. coli O157:H7 was detected on nonselective and selective media at log reductions of 1.08 to 1.25 after storage at 22 °C, 1.19 to 1.56 after storage at 4 °C, and 1.54 to 1.98 after storage at -18 °C. When the higher inoculation level of 10(3) CFU/100 ml was used, C. jejuni was able to survive at 22 and 4 °C, with 2.25- and 2.17-log reductions, respectively, observed on nonselective media. At these higher inoculation levels, E. coli O157:H7 was detectable at 22, 4, and -18 °C, with log reductions of 0.76, 0.97, and 1.21, respectively, achieved on nonselective media. Additionally, E. coli O157:H7 showed significant differences in culturability (P<0.05) on the nonselective and selective culture media under the different storage conditions, with storage at -18 °C for 2 days being the treatment most inhibiting. The percentage of sublethal injury of E. coli O157:H7 ranged from ∼33 to 75%, indicating that microbial examination of bottled water must be done carefully, otherwise false-negative results or underestimation of bacterial numbers could pose a health risk when low levels of pathogens are present." @default.
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- W2022802337 date "2011-02-01" @default.
- W2022802337 modified "2023-09-26" @default.
- W2022802337 title "Survival of Escherichia coli O157:H7 and Campylobacter jejuni in Bottled Purified Drinking Water under Different Storage Conditions" @default.
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- W2022802337 doi "https://doi.org/10.4315/0362-028x.jfp-10-368" @default.
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