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• W2022807614 abstract "Experiments of incorporation of a nucleolytic enzyme into human cells cultured in vitro have been carried out with the aim of inducing structural chromosome variations. Human heteroploid cells, either as asynchronous populations or enriched in mitoses, and PHA-stimulated lymphocytes were used as recipients. We found that all these cells when exposed to pancreatic DNAase I encapsulated in liposomes, either of multilamellar (MLV) or of small unilamellar (SUV) type, show an incidence of chromosome damage higher than that induced by the enzyme free in the incubation buffer. Our results indicate that liposomes are suitable vehicles for the transfer of an exogenous nuclease into human cultured cells. The enzyme remains functionally active and interacts with nuclear DNA, giving rise to chromosome lesions." @default.
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• W2022807614 date "1987-03-01" @default.
• W2022807614 modified "2023-09-30" @default.
• W2022807614 title "Chromosomal aberrations induced in human cultured cells by liposome-encapsulated deoxyribonuclease I" @default.
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• W2022807614 doi "https://doi.org/10.1016/0027-5107(87)90027-3" @default.
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