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- W2022807649 abstract "A method for the separation and quantification of indole-3-acetic acid and abscisic acid in Japanese chestnuts has been developed by use of Sephadex G-10 column chromatography and a reversed-phase high-performance liquid chromatography (HPLC). For the first purification step, the plant hormones are extracted with methanol—water (80:20) then eluted as individual hormone fractions from a Sephadex column. The final purification was performed on a reverse phase (LiChrosorb RP-18, 5 μm), eluted with a linear gradient of 10–50% acetonitrile in 1% glacial acetic acid. The column effluent is monitored at 254 nm. This technique has been used effectively to analyze plant hormones in biological samples." @default.
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- W2022807649 title "Determination of indole-3-acetic acid and abscisic acid in Japanese chestnuts by column chromatography on sephadex G-10 and reversed-phase high-performance liquid chromatography" @default.
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