FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022821292> ?p ?o ?g. }

• W2022821292 abstract "We established a retinal pigment epithelium-derived cell line from transgenic mouse harboring temperature-sensitive simian virus 40 T-antigen gene (tsSV40T) and examined its characteristics. We enucleated both eyes from a 2-month-old transgenic mouse and removed the retinal pigment epithelial (RPE) cells and neuroretinal cells. After cloning the RPE cells, we obtained a cell line (RPET). RPET cells grew well at 33 degrees C but not at 37 degrees C, expressing on the temperature-sensitive character of tsSV40T, and maintained characters of RPE cells such as T1-tyrosinase production, phagocytosis of rod outer segments, and presence of cytokeratin, microvilli on the cell surface and lysosome-like granules around the Golgi apparatus in the cytoplasm. Conditioned medium (CM) from a culture of neuroretinal cells harboring tsSV40T was essentially required for growth. The factor(s) in CM was heat-and acid labile, but was resistant to trypsin digestion. In the presence of 3% CM, epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and strong effects on RPET cells, whereas insulin, insulin-like growth factor I (IGF-I), and IGF-II had moderate growth effects. Interestingly, none of these growth factors stimulated the RPET cells in the absence of CM. EHS-Matrix had growth effect, whereas laminin, collagen types I and IV, and fibronectin had no marked growth effects on RPET cells. RPET cells were morphologically changed on a laminin-coated dish. They could not spread on the coated dish, and the majority of the cells floated. But when the floating cells were transferred to non-coated dishes, they immediately attached themselves. These results suggest that RPET cells are a good model for for finding novel growth factor(s) and for investigating the mechanism of cell-laminin attachment." @default.
• W2022821292 created "2016-06-24" @default.
• W2022821292 creator A5012926112 @default.
• W2022821292 creator A5036256585 @default.
• W2022821292 creator A5039225892 @default.
• W2022821292 creator A5039327799 @default.
• W2022821292 creator A5045711034 @default.
• W2022821292 creator A5063134169 @default.
• W2022821292 date "1996-01-01" @default.
• W2022821292 modified "2023-09-24" @default.
• W2022821292 title "A Reffnal Pigment Epithelium-derived Cell Line from Transgenic Mouse Harboring Temperature-sensitive Simian Virus 40 Large T-antigen Gene." @default.
• W2022821292 cites W103560028 @default.
• W2022821292 cites W142786351 @default.
• W2022821292 cites W143086085 @default.
• W2022821292 cites W152301937 @default.
• W2022821292 cites W1557023478 @default.
• W2022821292 cites W1874652601 @default.
• W2022821292 cites W1963591622 @default.
• W2022821292 cites W1964086351 @default.
• W2022821292 cites W1966600531 @default.
• W2022821292 cites W1969056236 @default.
• W2022821292 cites W1974997884 @default.
• W2022821292 cites W1975043689 @default.
• W2022821292 cites W1975986023 @default.
• W2022821292 cites W1978122258 @default.
• W2022821292 cites W1985332556 @default.
• W2022821292 cites W1985723318 @default.
• W2022821292 cites W1989698868 @default.
• W2022821292 cites W1992425768 @default.
• W2022821292 cites W2012465817 @default.
• W2022821292 cites W2012557091 @default.
• W2022821292 cites W2016648084 @default.
• W2022821292 cites W2020451017 @default.
• W2022821292 cites W2029231027 @default.
• W2022821292 cites W2031970538 @default.
• W2022821292 cites W2033269379 @default.
• W2022821292 cites W2041343040 @default.
• W2022821292 cites W2048948497 @default.
• W2022821292 cites W2051747007 @default.
• W2022821292 cites W2054860528 @default.
• W2022821292 cites W2062523292 @default.
• W2022821292 cites W2071806319 @default.
• W2022821292 cites W2085489324 @default.
• W2022821292 cites W2086790354 @default.
• W2022821292 cites W2088703323 @default.
• W2022821292 cites W2109843381 @default.
• W2022821292 cites W2132180445 @default.
• W2022821292 cites W2137454157 @default.
• W2022821292 cites W2140083987 @default.
• W2022821292 cites W2145539666 @default.
• W2022821292 cites W2163577842 @default.
• W2022821292 cites W2169265616 @default.
• W2022821292 cites W2257098168 @default.
• W2022821292 cites W2278844756 @default.
• W2022821292 cites W2410098848 @default.
• W2022821292 cites W49997331 @default.
• W2022821292 cites W77099808 @default.
• W2022821292 cites W80027751 @default.
• W2022821292 doi "https://doi.org/10.1247/csf.21.459" @default.
• W2022821292 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9078403" @default.
• W2022821292 hasPublicationYear "1996" @default.
• W2022821292 type Work @default.
• W2022821292 sameAs 2022821292 @default.
• W2022821292 citedByCount "8" @default.
• W2022821292 countsByYear W20228212922015 @default.
• W2022821292 crossrefType "journal-article" @default.
• W2022821292 hasAuthorship W2022821292A5012926112 @default.
• W2022821292 hasAuthorship W2022821292A5036256585 @default.
• W2022821292 hasAuthorship W2022821292A5039225892 @default.
• W2022821292 hasAuthorship W2022821292A5039327799 @default.
• W2022821292 hasAuthorship W2022821292A5045711034 @default.
• W2022821292 hasAuthorship W2022821292A5063134169 @default.
• W2022821292 hasBestOaLocation W20228212921 @default.
• W2022821292 hasConcept C153911025 @default.
• W2022821292 hasConcept C170493617 @default.
• W2022821292 hasConcept C189165786 @default.
• W2022821292 hasConcept C2775960820 @default.
• W2022821292 hasConcept C2776362946 @default.
• W2022821292 hasConcept C2776996007 @default.
• W2022821292 hasConcept C2777587049 @default.
• W2022821292 hasConcept C2778024200 @default.
• W2022821292 hasConcept C2779814568 @default.
• W2022821292 hasConcept C2780827179 @default.
• W2022821292 hasConcept C54355233 @default.
• W2022821292 hasConcept C55493867 @default.
• W2022821292 hasConcept C81885089 @default.
• W2022821292 hasConcept C86803240 @default.
• W2022821292 hasConcept C95444343 @default.
• W2022821292 hasConceptScore W2022821292C153911025 @default.
• W2022821292 hasConceptScore W2022821292C170493617 @default.
• W2022821292 hasConceptScore W2022821292C189165786 @default.
• W2022821292 hasConceptScore W2022821292C2775960820 @default.
• W2022821292 hasConceptScore W2022821292C2776362946 @default.
• W2022821292 hasConceptScore W2022821292C2776996007 @default.
• W2022821292 hasConceptScore W2022821292C2777587049 @default.
• W2022821292 hasConceptScore W2022821292C2778024200 @default.
• W2022821292 hasConceptScore W2022821292C2779814568 @default.
• W2022821292 hasConceptScore W2022821292C2780827179 @default.
• W2022821292 hasConceptScore W2022821292C54355233 @default.
• W2022821292 hasConceptScore W2022821292C55493867 @default.

• next