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- W2022825062 endingPage "444" @default.
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- W2022825062 abstract "Utilizing Marathon‐ready cDNA library and a gene‐specific primer corresponding to a partial amino acid sequence determined previously, the complete nucleotide sequence for the cDNA of crocalbin, which binds crotoxin (a phospholipase A 2 ) and Ca 2+ , was obtained by polymerase chain reaction. The open reading frame of the cDNA encodes a novel polypeptide of 315 amino acid residues, including a signal sequence of 19 residues. This protein contains six potential Ca 2+ ‐binding domains, one N ‐glycosylation site, and a large amount of acidic amino acid residues. The ability to bind Ca 2+ has been ascertained by calcium overlay experiment. Evidenced by sequence similarity in addition, it is concluded that crocalbin is a new member of the reticulocalbin family of calcium‐binding proteins." @default.
- W2022825062 created "2016-06-24" @default.
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- W2022825062 creator A5066777156 @default.
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- W2022825062 date "1999-02-26" @default.
- W2022825062 modified "2023-10-18" @default.
- W2022825062 title "Crocalbin: a new calcium‐binding protein that is also a binding protein for crotoxin, a neurotoxic phospholipase A<sub>2</sub>" @default.
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- W2022825062 doi "https://doi.org/10.1016/s0014-5793(99)00177-5" @default.
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