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- W2022830140 abstract "A new indirect inhibitive immunoassay using surface plasmon resonance (SPR) coupled with molecularly imprinted polymer (MIP) was developed and applied for the analysis of sulfamethoxazole (SMX). A SMX MIP coating was synthesized in a flexible quartz capillary (30 cm × 0.25 mm i.d.) by in situ polymerization technique. The thickness of the coating was 198 nm illustrated by scanning electron microscope (SEM) image. The detection was conducted on BIAcore 3000 sensor automatically. The MIP-coating capillary was used as online solid phase extraction (SPE) tube before SPR detection. The anti-SMX mono-antibody was inhibited by SMX that was extracted by the MIP coating in a dose-dependent manner. The calibration curve was generated by linear fit in the range of 0.04–10.0 μg L−1. The detection limit of SMX was 0.01 μg L−1. It was directly applied for the analysis of real-world samples. This method has high sensitivity and can be performed automatically." @default.
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- W2022830140 date "2010-07-01" @default.
- W2022830140 modified "2023-10-18" @default.
- W2022830140 title "An Indirect Inhibitive Immunoassay for Detection of Low Concentration Sulfamethoxazole in Aqueous Solution" @default.
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- W2022830140 doi "https://doi.org/10.1016/s1872-2040(09)60058-x" @default.
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