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- W2022831988 abstract "Conformational transitions of horse heart ferricytochrome c (ferricyt c ) have been investigated in the presence of strong inorganic acids and their salts by optical absorption spectroscopy, magnetic circular dichroism and circular dichroism. In the presence of acids (HClO 4 or H 2 SO 4 , pH 2) or their salts (1 M NaClO 4 or Na 2 SO 4 , pH 2, 25 °C), the three ligation states of ferricyt c heme were identified. One is the high-spin state: His18-Fe-H 2 O (40-50%), and two are the low-spin states: His18-Fe-Met80 (30-25%) and His18-Fe-His (30-25%). Under these conditions low temperatures facilitate native heme coordination of ferricyt c . Transition from low-spin to high-spin heme coordination of ferricyt c is complete in 1 M HClO 4 or 3 M H 2 SO 4 . At the concentration of HClO 4 and H 2 SO 4 above 3 M, different behavior in spectral transitions of ferricyt c near the heme is observed. High-spin pentacoordinated ferricyt c with the heme ligand of His18-Fe is formed in 8 M H 2 SO 4 . This state is unstable at higher concentration of H 2 SO 4 and porphyrin ferricyt c is formed. At HClO 4 concentration higher than 3 M, the new, until this time not observed heme coordination structure of ferricyt c originates." @default.
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- W2022831988 date "2006-01-01" @default.
- W2022831988 modified "2023-10-17" @default.
- W2022831988 title "Conformational Transitions of Ferricytochrome c in Strong Inorganic Acids" @default.
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- W2022831988 doi "https://doi.org/10.1135/cccc20061627" @default.
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