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- W2022832051 abstract "Abstract New pathways leading to a better understanding of the cell nucleus morpho-functional aspects are being opened up by cryofixation and cryosubstitution methods. These techniques are able to produce excellent morphology of the nuclear fine structure with subtle, sometimes even notable, ultrastructural differences when compared with conventional chemically fixed, dehydrated and embedded material. It is of particular interest, when carrying out these cryotechniques, to avoid the use of any cryoprotectant and chemical fixation. This permits an analysis of the cell's fine structure, which may well approach its native state. Cryofixation, because of its extreme rapidity, should be able to immobilize all cell activities and capture a well defined physiological state of the cell without causing major changes in molecular structures. Immunocrytochemical studies carried out using cryofixation and cryosubstitution, gave very satisfactory results, often revealing antigenic sites which were not, or hardly ever, detected when using conventional fixation techniques. The applications of various cryofixation and cryosubstitution methods in the study of the cell nucleus are summarized and an attempt to critically evaluate their results is made in this review." @default.
- W2022832051 created "2016-06-24" @default.
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- W2022832051 date "1993-01-01" @default.
- W2022832051 modified "2023-09-26" @default.
- W2022832051 title "The study of the cell nucleus using cryofixation and cryosubstitution" @default.
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- W2022832051 doi "https://doi.org/10.1016/0968-4328(93)90027-x" @default.
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