FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022846914> ?p ?o ?g. }

• W2022846914 endingPage "5473" @default.
• W2022846914 startingPage "5464" @default.
• W2022846914 abstract "The bacteriophage P1 Cre/loxP site-specific recombination system is a useful tool in a number of genetic engineering processes. The Cre recombinase has been shown to act on DNA sequences that vary considerably from that of its bacteriophage recognition sequence, loxP. However, little is known about the sequence requirements for functional lox-like sequences. In this study, we have implemented a randomized library approach to identify the sequence characteristics of functional lox site domains. We created a randomized spacer library and a randomized arm library, and then tested them for recombination in vivo and in vitro. Results from the spacer library show that, while there is great plasticity, identity between spacer pairs is the most important factor influencing function, especially in in vitro reactions. The presence of one completely randomized arm in a functional loxP recombination reaction revealed that only three wild-type loxP arms are necessary for successful recombination in Cre-expressing bacteria, and that there are nucleotide preferences at the first three and last three positions of the randomized arm for the most efficiently recombined sequences. Finally, we found that in vitro Cre recombination reactions are much more stringent for evaluating which sequences can support efficient recombination compared to the 294-CRE system." @default.
• W2022846914 created "2016-06-24" @default.
• W2022846914 creator A5047381478 @default.
• W2022846914 creator A5058303202 @default.
• W2022846914 creator A5085095319 @default.
• W2022846914 date "2007-08-17" @default.
• W2022846914 modified "2023-09-23" @default.
• W2022846914 title "A randomized library approach to identifying functional lox site domains for the Cre recombinase" @default.
• W2022846914 cites W1500382785 @default.
• W2022846914 cites W1556784368 @default.
• W2022846914 cites W1586539673 @default.
• W2022846914 cites W1590546489 @default.
• W2022846914 cites W1601944560 @default.
• W2022846914 cites W1678580492 @default.
• W2022846914 cites W1968541952 @default.
• W2022846914 cites W1976918496 @default.
• W2022846914 cites W1983188063 @default.
• W2022846914 cites W1984242216 @default.
• W2022846914 cites W1988309865 @default.
• W2022846914 cites W1989460113 @default.
• W2022846914 cites W1991830373 @default.
• W2022846914 cites W1999563094 @default.
• W2022846914 cites W2002581509 @default.
• W2022846914 cites W2002669389 @default.
• W2022846914 cites W2007414034 @default.
• W2022846914 cites W2018168127 @default.
• W2022846914 cites W2020024953 @default.
• W2022846914 cites W2020777893 @default.
• W2022846914 cites W2022245153 @default.
• W2022846914 cites W2029397598 @default.
• W2022846914 cites W2038970416 @default.
• W2022846914 cites W2045729962 @default.
• W2022846914 cites W2048139403 @default.
• W2022846914 cites W2053200644 @default.
• W2022846914 cites W2055859583 @default.
• W2022846914 cites W2065238750 @default.
• W2022846914 cites W2072761181 @default.
• W2022846914 cites W2084773215 @default.
• W2022846914 cites W2086791288 @default.
• W2022846914 cites W2094853557 @default.
• W2022846914 cites W2095310293 @default.
• W2022846914 cites W2104854307 @default.
• W2022846914 cites W2119095571 @default.
• W2022846914 cites W2123311560 @default.
• W2022846914 doi "https://doi.org/10.1093/nar/gkm604" @default.
• W2022846914 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2018622" @default.
• W2022846914 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17702764" @default.
• W2022846914 hasPublicationYear "2007" @default.
• W2022846914 type Work @default.
• W2022846914 sameAs 2022846914 @default.
• W2022846914 citedByCount "30" @default.
• W2022846914 countsByYear W20228469142012 @default.
• W2022846914 countsByYear W20228469142013 @default.
• W2022846914 countsByYear W20228469142014 @default.
• W2022846914 countsByYear W20228469142015 @default.
• W2022846914 countsByYear W20228469142016 @default.
• W2022846914 countsByYear W20228469142017 @default.
• W2022846914 countsByYear W20228469142018 @default.
• W2022846914 countsByYear W20228469142019 @default.
• W2022846914 countsByYear W20228469142021 @default.
• W2022846914 countsByYear W20228469142022 @default.
• W2022846914 crossrefType "journal-article" @default.
• W2022846914 hasAuthorship W2022846914A5047381478 @default.
• W2022846914 hasAuthorship W2022846914A5058303202 @default.
• W2022846914 hasAuthorship W2022846914A5085095319 @default.
• W2022846914 hasBestOaLocation W20228469141 @default.
• W2022846914 hasConcept C101434241 @default.
• W2022846914 hasConcept C102230213 @default.
• W2022846914 hasConcept C102414288 @default.
• W2022846914 hasConcept C104317684 @default.
• W2022846914 hasConcept C119898374 @default.
• W2022846914 hasConcept C125929170 @default.
• W2022846914 hasConcept C135300049 @default.
• W2022846914 hasConcept C141035611 @default.
• W2022846914 hasConcept C156695909 @default.
• W2022846914 hasConcept C2776441376 @default.
• W2022846914 hasConcept C38027674 @default.
• W2022846914 hasConcept C54355233 @default.
• W2022846914 hasConcept C547475151 @default.
• W2022846914 hasConcept C552990157 @default.
• W2022846914 hasConcept C70721500 @default.
• W2022846914 hasConcept C86803240 @default.
• W2022846914 hasConceptScore W2022846914C101434241 @default.
• W2022846914 hasConceptScore W2022846914C102230213 @default.
• W2022846914 hasConceptScore W2022846914C102414288 @default.
• W2022846914 hasConceptScore W2022846914C104317684 @default.
• W2022846914 hasConceptScore W2022846914C119898374 @default.
• W2022846914 hasConceptScore W2022846914C125929170 @default.
• W2022846914 hasConceptScore W2022846914C135300049 @default.
• W2022846914 hasConceptScore W2022846914C141035611 @default.
• W2022846914 hasConceptScore W2022846914C156695909 @default.
• W2022846914 hasConceptScore W2022846914C2776441376 @default.
• W2022846914 hasConceptScore W2022846914C38027674 @default.
• W2022846914 hasConceptScore W2022846914C54355233 @default.
• W2022846914 hasConceptScore W2022846914C547475151 @default.
• W2022846914 hasConceptScore W2022846914C552990157 @default.
• W2022846914 hasConceptScore W2022846914C70721500 @default.
• W2022846914 hasConceptScore W2022846914C86803240 @default.

• next