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• W2022872499 abstract "Microbodies (peroxisomes and glyoxysomes), mitochondria, and microsomes from rat liver, dog kidney, spinach leaves sunflower cotyledons, and castor bean endosperm were isolated by sucrose density-gradient centrifugation. The microbody-limiting membrane and microsomes each contained NADH-cytochrome c reductase and had a similar phospholipid composition. NADH-cytochrome c reductase from plant and animal microbodies and microsomes was insensitive to antimycin A, which inhibited the activity in the mitochondrial fractions. The pH optima of cytochrome c reductase in plant microbodies and microsomes was 7.5–9.0, which was 2 pH units higher than the optima for the mitochondrial form of the enzyme. The activity in animal organelles exhibited a broad pH optimum between pH 6 and 9. Rat liver peroxisomes retained cytochrome c reductase activity, when diluted with water, KCl, or EDTA solutions and reisolated. Cytochrome c reductase activity of microbodies was lost upon disruption by digitonin or Triton X-100, but other peroxisomal enzymes of the matrix were not destroyed. The microbody fraction from each tissue also contained a small amount of NADH-cytochrome b5 reductase activity. Peroxisomes from spinach leaves were broken by osmotic shock and particles from rat liver by diluting in alkaline pyrophosphate. Upon recentrifugation liver peroxisomes yielded a core fraction containing urate oxidase at a sucrose gradient density of 1.23 g × cm−3, a membrane fraction at 1.17 g × cm−3 containing NADH-cytochrome c reductase, and soluble matrix enzymes at the top of the gradient. Relative to other organelles the microbodies had a very low level of phospholipid (0.03–0.09 mg per mg protein). Phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol, and phosphatidyl serine were found in all organelle fractions. The percentage of each phospholipid in isolated microbodies from rat liver or spinach leaves was similar in the microbodies and microsomes. Glyoxysomes from germinating castor beans contained little phosphatidyl serine but rather an unidentified phospholipid. From spinach leaves the microsomes contained much phosphatidyl serine and the mitochondria had a significant amount of cardiolipin." @default.
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• W2022872499 date "1972-09-01" @default.
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• W2022872499 title "A comparison of microbody membranes with microsomes and mitochondria from plant and animal tissue" @default.
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• W2022872499 doi "https://doi.org/10.1016/0003-9861(72)90208-1" @default.
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