FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022873110> ?p ?o ?g. }

• W2022873110 endingPage "207" @default.
• W2022873110 startingPage "202" @default.
• W2022873110 abstract "Altered DNA methylation has been linked to neoplastic cell transformation and is a hallmark of cancer progression. Therefore, the screening for differentially methylated sequences as tumor biomarkers has a significant implication in the clinical setting. To determine the cancer-linked alterations in DNA methylation pattern, we have applied an endonuclease, McrBC, to the existing methylation-sensitive arbitrarily primed polymerase chain reaction (msAP-PCR) method and developed McrBC-msAP-PCR. This modified approach allows detection of differentially methylated sites within unmethylated DNA domains enriched by regulatory sequences and CpG islands. In this method, we used digestion of DNA with the McrBC methylation-sensitive endonuclease to selectively exclude the methylated fraction of DNA, which comprises interspersed and tandem-repeated sequences and exons other than first exons, from analysis. The subsequent digestion of unmethylated DNA fragments with SmaI and HpaII methylation-sensitive restriction endonucleases followed by AP-PCR amplification resulted in the detection of unknown unique sequences associated with cancer-linked methylation changes in genomic DNA. Hypermethylation and hypomethylation are visualized by the increase or decrease in the band intensity of DNA fingerprints. By using this technique, we were able to differentiate clearly, identify, and characterize a number of novel unique DNA sequences with differentially methylated sites in normal and breast cancer cell lines and in normal and rat tumor liver tissues." @default.
• W2022873110 created "2016-06-24" @default.
• W2022873110 creator A5001137259 @default.
• W2022873110 creator A5015793767 @default.
• W2022873110 creator A5062637673 @default.
• W2022873110 date "2006-09-01" @default.
• W2022873110 modified "2023-09-25" @default.
• W2022873110 title "Identification of differentially methylated sites within unmethylated DNA domains in normal and cancer cells" @default.
• W2022873110 cites W1504303645 @default.
• W2022873110 cites W1755052449 @default.
• W2022873110 cites W1969073965 @default.
• W2022873110 cites W1969727351 @default.
• W2022873110 cites W1973745530 @default.
• W2022873110 cites W1974747095 @default.
• W2022873110 cites W1985827318 @default.
• W2022873110 cites W1990381590 @default.
• W2022873110 cites W2004172747 @default.
• W2022873110 cites W2012976264 @default.
• W2022873110 cites W2018063523 @default.
• W2022873110 cites W2029874395 @default.
• W2022873110 cites W2034641677 @default.
• W2022873110 cites W2036300762 @default.
• W2022873110 cites W2040149948 @default.
• W2022873110 cites W2043033879 @default.
• W2022873110 cites W2053616835 @default.
• W2022873110 cites W2067003561 @default.
• W2022873110 cites W2079476845 @default.
• W2022873110 cites W2087676315 @default.
• W2022873110 cites W2097040991 @default.
• W2022873110 cites W2098203176 @default.
• W2022873110 cites W2101259235 @default.
• W2022873110 cites W2103163028 @default.
• W2022873110 cites W2111584908 @default.
• W2022873110 cites W2118062759 @default.
• W2022873110 cites W2128107471 @default.
• W2022873110 cites W2146872344 @default.
• W2022873110 cites W2154929782 @default.
• W2022873110 cites W2157985757 @default.
• W2022873110 cites W2162292699 @default.
• W2022873110 cites W2166319541 @default.
• W2022873110 cites W2168909179 @default.
• W2022873110 doi "https://doi.org/10.1016/j.ab.2006.05.019" @default.
• W2022873110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16824473" @default.
• W2022873110 hasPublicationYear "2006" @default.
• W2022873110 type Work @default.
• W2022873110 sameAs 2022873110 @default.
• W2022873110 citedByCount "24" @default.
• W2022873110 countsByYear W20228731102012 @default.
• W2022873110 countsByYear W20228731102013 @default.
• W2022873110 countsByYear W20228731102015 @default.
• W2022873110 countsByYear W20228731102018 @default.
• W2022873110 countsByYear W20228731102019 @default.
• W2022873110 countsByYear W20228731102021 @default.
• W2022873110 crossrefType "journal-article" @default.
• W2022873110 hasAuthorship W2022873110A5001137259 @default.
• W2022873110 hasAuthorship W2022873110A5015793767 @default.
• W2022873110 hasAuthorship W2022873110A5062637673 @default.
• W2022873110 hasConcept C104317684 @default.
• W2022873110 hasConcept C116114238 @default.
• W2022873110 hasConcept C128319531 @default.
• W2022873110 hasConcept C136953436 @default.
• W2022873110 hasConcept C140173407 @default.
• W2022873110 hasConcept C150194340 @default.
• W2022873110 hasConcept C153911025 @default.
• W2022873110 hasConcept C154253233 @default.
• W2022873110 hasConcept C17757408 @default.
• W2022873110 hasConcept C190727270 @default.
• W2022873110 hasConcept C33288867 @default.
• W2022873110 hasConcept C54355233 @default.
• W2022873110 hasConcept C552990157 @default.
• W2022873110 hasConcept C86803240 @default.
• W2022873110 hasConceptScore W2022873110C104317684 @default.
• W2022873110 hasConceptScore W2022873110C116114238 @default.
• W2022873110 hasConceptScore W2022873110C128319531 @default.
• W2022873110 hasConceptScore W2022873110C136953436 @default.
• W2022873110 hasConceptScore W2022873110C140173407 @default.
• W2022873110 hasConceptScore W2022873110C150194340 @default.
• W2022873110 hasConceptScore W2022873110C153911025 @default.
• W2022873110 hasConceptScore W2022873110C154253233 @default.
• W2022873110 hasConceptScore W2022873110C17757408 @default.
• W2022873110 hasConceptScore W2022873110C190727270 @default.
• W2022873110 hasConceptScore W2022873110C33288867 @default.
• W2022873110 hasConceptScore W2022873110C54355233 @default.
• W2022873110 hasConceptScore W2022873110C552990157 @default.
• W2022873110 hasConceptScore W2022873110C86803240 @default.
• W2022873110 hasIssue "2" @default.
• W2022873110 hasLocation W20228731101 @default.
• W2022873110 hasLocation W20228731102 @default.
• W2022873110 hasOpenAccess W2022873110 @default.
• W2022873110 hasPrimaryLocation W20228731101 @default.
• W2022873110 hasRelatedWork W1969014507 @default.
• W2022873110 hasRelatedWork W1997684837 @default.
• W2022873110 hasRelatedWork W2053397449 @default.
• W2022873110 hasRelatedWork W2083886218 @default.
• W2022873110 hasRelatedWork W2149655654 @default.
• W2022873110 hasRelatedWork W2153751762 @default.
• W2022873110 hasRelatedWork W2313849049 @default.
• W2022873110 hasRelatedWork W2382375971 @default.

• next