FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022873560> ?p ?o ?g. }

• W2022873560 endingPage "e2696" @default.
• W2022873560 startingPage "e2696" @default.
• W2022873560 abstract "Trypanosoma cruzi calreticulin (TcCRT) is a virulence factor that binds complement C1, thus inhibiting the activation of the classical complement pathway and generating pro-phagocytic signals that increase parasite infectivity. In a previous work, we characterized a clonal cell line lacking one TcCRT allele (TcCRT+/−) and another overexpressing it (TcCRT+), both derived from the attenuated TCC T. cruzi strain. The TcCRT+/− mutant was highly susceptible to killing by the complement machinery and presented a remarkable reduced propagation and differentiation rate both in vitro and in vivo. In this report, we have extended these studies to assess, in a mouse model of disease, the virulence, immunogenicity and safety of the mutant as an experimental vaccine. Balb/c mice were inoculated with TcCRT+/− parasites and followed-up during a 6-month period. Mutant parasites were not detected by sensitive techniques, even after mice immune suppression. Total anti-T. cruzi IgG levels were undetectable in TcCRT+/− inoculated mice and the genetic alteration was stable after long-term infection and it did not revert back to wild type form. Most importantly, immunization with TcCRT+/− parasites induces a highly protective response after challenge with a virulent T. cruzi strain, as evidenced by lower parasite density, mortality, spleen index and tissue inflammatory response. TcCRT+/− clones are restricted in two important properties conferred by TcCRT and indirectly by C1q: their ability to evade the host immune response and their virulence. Therefore, deletion of one copy of the TcCRT gene in the attenuated TCC strain generated a safe and irreversibly gene-deleted live attenuated parasite with high immunoprotective properties. Our results also contribute to endorse the important role of TcCRT as a T. cruzi virulence factor." @default.
• W2022873560 created "2016-06-24" @default.
• W2022873560 creator A5007357728 @default.
• W2022873560 creator A5016675411 @default.
• W2022873560 creator A5023496894 @default.
• W2022873560 creator A5029979309 @default.
• W2022873560 creator A5033236138 @default.
• W2022873560 creator A5037237697 @default.
• W2022873560 creator A5047899126 @default.
• W2022873560 creator A5050106987 @default.
• W2022873560 creator A5054394057 @default.
• W2022873560 creator A5078782756 @default.
• W2022873560 date "2014-02-13" @default.
• W2022873560 modified "2023-10-16" @default.
• W2022873560 title "A Monoallelic Deletion of the TcCRT Gene Increases the Attenuation of a Cultured Trypanosoma cruzi Strain, Protecting against an In Vivo Virulent Challenge" @default.
• W2022873560 cites W1874018940 @default.
• W2022873560 cites W1912914184 @default.
• W2022873560 cites W1972043575 @default.
• W2022873560 cites W1973650923 @default.
• W2022873560 cites W1977152655 @default.
• W2022873560 cites W1978797291 @default.
• W2022873560 cites W1997188707 @default.
• W2022873560 cites W2001937023 @default.
• W2022873560 cites W2017148692 @default.
• W2022873560 cites W2019809965 @default.
• W2022873560 cites W2023207791 @default.
• W2022873560 cites W2028307436 @default.
• W2022873560 cites W2030803676 @default.
• W2022873560 cites W2032980181 @default.
• W2022873560 cites W2034870592 @default.
• W2022873560 cites W2035019807 @default.
• W2022873560 cites W2039481952 @default.
• W2022873560 cites W2042528617 @default.
• W2022873560 cites W2049264972 @default.
• W2022873560 cites W2055361966 @default.
• W2022873560 cites W2058261647 @default.
• W2022873560 cites W2062137938 @default.
• W2022873560 cites W2068012504 @default.
• W2022873560 cites W2068843087 @default.
• W2022873560 cites W2069128133 @default.
• W2022873560 cites W2070854001 @default.
• W2022873560 cites W2076162694 @default.
• W2022873560 cites W2079749183 @default.
• W2022873560 cites W2080657619 @default.
• W2022873560 cites W2091651330 @default.
• W2022873560 cites W2095693566 @default.
• W2022873560 cites W2099983818 @default.
• W2022873560 cites W2100151338 @default.
• W2022873560 cites W2100907409 @default.
• W2022873560 cites W2102089743 @default.
• W2022873560 cites W2105573880 @default.
• W2022873560 cites W2109473935 @default.
• W2022873560 cites W2113554363 @default.
• W2022873560 cites W2122795797 @default.
• W2022873560 cites W2136384724 @default.
• W2022873560 cites W2136953905 @default.
• W2022873560 cites W2152399276 @default.
• W2022873560 cites W2162355257 @default.
• W2022873560 cites W2174379977 @default.
• W2022873560 cites W2179573766 @default.
• W2022873560 cites W2319095344 @default.
• W2022873560 cites W2338770458 @default.
• W2022873560 cites W2412570511 @default.
• W2022873560 doi "https://doi.org/10.1371/journal.pntd.0002696" @default.
• W2022873560 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3923724" @default.
• W2022873560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24551259" @default.
• W2022873560 hasPublicationYear "2014" @default.
• W2022873560 type Work @default.
• W2022873560 sameAs 2022873560 @default.
• W2022873560 citedByCount "21" @default.
• W2022873560 countsByYear W20228735602014 @default.
• W2022873560 countsByYear W20228735602015 @default.
• W2022873560 countsByYear W20228735602016 @default.
• W2022873560 countsByYear W20228735602017 @default.
• W2022873560 countsByYear W20228735602018 @default.
• W2022873560 countsByYear W20228735602019 @default.
• W2022873560 countsByYear W20228735602020 @default.
• W2022873560 countsByYear W20228735602021 @default.
• W2022873560 countsByYear W20228735602022 @default.
• W2022873560 countsByYear W20228735602023 @default.
• W2022873560 crossrefType "journal-article" @default.
• W2022873560 hasAuthorship W2022873560A5007357728 @default.
• W2022873560 hasAuthorship W2022873560A5016675411 @default.
• W2022873560 hasAuthorship W2022873560A5023496894 @default.
• W2022873560 hasAuthorship W2022873560A5029979309 @default.
• W2022873560 hasAuthorship W2022873560A5033236138 @default.
• W2022873560 hasAuthorship W2022873560A5037237697 @default.
• W2022873560 hasAuthorship W2022873560A5047899126 @default.
• W2022873560 hasAuthorship W2022873560A5050106987 @default.
• W2022873560 hasAuthorship W2022873560A5054394057 @default.
• W2022873560 hasAuthorship W2022873560A5078782756 @default.
• W2022873560 hasBestOaLocation W20228735601 @default.
• W2022873560 hasConcept C104317684 @default.
• W2022873560 hasConcept C106358424 @default.
• W2022873560 hasConcept C111684460 @default.
• W2022873560 hasConcept C112418997 @default.
• W2022873560 hasConcept C136764020 @default.
• W2022873560 hasConcept C143065580 @default.

• next