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• W2022879923 abstract "To investigate the effects of benzalkonium chloride (BAK) on the human reconstituted corneal epithelial model (HCE) and to optimize the operating potential of this model in the field of ophthalmic toxicology.The HCEs were treated with 0.001% to 0.5% BAK for 24 hours followed or not by a 24-hour postincubation period. To complete the histologic analysis, the authors designed a new MTT procedure to assess cellular viability. Frozen sections were analyzed by using fluorescence confocal microscopy for the presence of TUNEL, activated caspase-3, Ki67, ICAM-1, HLA-DR, E-cadherin, and occludin. Occludin gene expression was also investigated by using quantitative RT-PCR.The MTT test revealed a dose-dependent response of BAK with significant toxic effects for concentrations as low as 0.005%. Increasing BAK concentrations induced an increased number of apoptotic cells, found from the superficial to the deeper layers, with the activation of caspase-3 at 0.01% and 0.02% concentrations. The number of Ki67- and ICAM-1-positive cells increased with 0.01% BAK and with 0.001% to 0.01% BAK, respectively. BAK induced the dose-dependent disappearance of occludin in the superficial layers while increasing its gene expression up to the 0.02% BAK concentration.Fluorescence techniques conjugated with confocal microscopy on 3D-reconstructed corneal epithelia were well suited for the investigation of toxicological markers such as cell junction alteration, apoptosis, cell activation, and proliferation and gave relevant results compared with the known human data. They complement the new sensitive MTT test and improve the operating potential of this new, valuable 3D model in ophthalmic toxicology." @default.
• W2022879923 created "2016-06-24" @default.
• W2022879923 creator A5018656902 @default.
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• W2022879923 date "2009-04-01" @default.
• W2022879923 modified "2023-10-16" @default.
• W2022879923 title "Multiple Endpoint Analysis of the 3D-Reconstituted Corneal Epithelium after Treatment with Benzalkonium Chloride: Early Detection of Toxic Damage" @default.
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• W2022879923 cites W1502075302 @default.
• W2022879923 cites W1508331875 @default.
• W2022879923 cites W1537855055 @default.
• W2022879923 cites W1541538097 @default.
• W2022879923 cites W1560435938 @default.
• W2022879923 cites W158559211 @default.
• W2022879923 cites W1658076554 @default.
• W2022879923 cites W1736987190 @default.
• W2022879923 cites W185887293 @default.
• W2022879923 cites W189381086 @default.
• W2022879923 cites W1964226830 @default.
• W2022879923 cites W1970704125 @default.
• W2022879923 cites W1970731633 @default.
• W2022879923 cites W1977206451 @default.
• W2022879923 cites W1983672529 @default.
• W2022879923 cites W1984282789 @default.
• W2022879923 cites W1984656953 @default.
• W2022879923 cites W1990090315 @default.
• W2022879923 cites W1990526387 @default.
• W2022879923 cites W1991609949 @default.
• W2022879923 cites W2003336901 @default.
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• W2022879923 cites W2009259670 @default.
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• W2022879923 cites W2014579944 @default.
• W2022879923 cites W2018594714 @default.
• W2022879923 cites W2018885276 @default.
• W2022879923 cites W2019688875 @default.
• W2022879923 cites W2032016124 @default.
• W2022879923 cites W2034113249 @default.
• W2022879923 cites W2042478318 @default.
• W2022879923 cites W2043204214 @default.
• W2022879923 cites W2044783794 @default.
• W2022879923 cites W2047223915 @default.
• W2022879923 cites W2047324342 @default.
• W2022879923 cites W2048744275 @default.
• W2022879923 cites W2051912691 @default.
• W2022879923 cites W2058122061 @default.
• W2022879923 cites W2066367825 @default.
• W2022879923 cites W2076111934 @default.
• W2022879923 cites W2078022305 @default.
• W2022879923 cites W2079381455 @default.
• W2022879923 cites W2079544843 @default.
• W2022879923 cites W2080276945 @default.
• W2022879923 cites W2080873175 @default.
• W2022879923 cites W2082206820 @default.
• W2022879923 cites W2082340321 @default.
• W2022879923 cites W2089286833 @default.
• W2022879923 cites W2090719004 @default.
• W2022879923 cites W2093808180 @default.
• W2022879923 cites W2106421222 @default.
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• W2022879923 cites W2141819648 @default.
• W2022879923 cites W2164561539 @default.
• W2022879923 cites W2172160601 @default.
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• W2022879923 doi "https://doi.org/10.1167/iovs.08-2992" @default.
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• W2022879923 hasPublicationYear "2009" @default.
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