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• W2022889556 abstract "Article29 November 2007free access Neural recognition molecules CHL1 and NB-3 regulate apical dendrite orientation in the neocortex via PTPα Haihong Ye Haihong Ye Institute of Molecular and Cell Biology, Singapore Search for more papers by this author Yen Ling Jessie Tan Yen Ling Jessie Tan Institute of Molecular and Cell Biology, Singapore Search for more papers by this author Sathivel Ponniah Sathivel Ponniah Singapore Search for more papers by this author Yasuo Takeda Yasuo Takeda Department of Clinical Pharmacy and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka, Kagoshima, Japan Search for more papers by this author Shi-Qiang Wang Shi-Qiang Wang State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University College of Life Sciences, Beijing, China Search for more papers by this author Melitta Schachner Melitta Schachner Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA Sino-German Center for Neuroscience, Dalian Medical University, Dalian, China Search for more papers by this author Kazutada Watanabe Kazutada Watanabe Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan Search for more papers by this author Catherine J Pallen Catherine J Pallen Department of Pediatrics, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada Search for more papers by this author Zhi-Cheng Xiao Corresponding Author Zhi-Cheng Xiao Institute of Molecular and Cell Biology, Singapore Department of Clinical Research, Singapore General Hospital, Singapore Search for more papers by this author Haihong Ye Haihong Ye Institute of Molecular and Cell Biology, Singapore Search for more papers by this author Yen Ling Jessie Tan Yen Ling Jessie Tan Institute of Molecular and Cell Biology, Singapore Search for more papers by this author Sathivel Ponniah Sathivel Ponniah Singapore Search for more papers by this author Yasuo Takeda Yasuo Takeda Department of Clinical Pharmacy and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka, Kagoshima, Japan Search for more papers by this author Shi-Qiang Wang Shi-Qiang Wang State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University College of Life Sciences, Beijing, China Search for more papers by this author Melitta Schachner Melitta Schachner Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA Sino-German Center for Neuroscience, Dalian Medical University, Dalian, China Search for more papers by this author Kazutada Watanabe Kazutada Watanabe Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan Search for more papers by this author Catherine J Pallen Catherine J Pallen Department of Pediatrics, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada Search for more papers by this author Zhi-Cheng Xiao Corresponding Author Zhi-Cheng Xiao Institute of Molecular and Cell Biology, Singapore Department of Clinical Research, Singapore General Hospital, Singapore Search for more papers by this author Author Information Haihong Ye1, Yen Ling Jessie Tan1, Sathivel Ponniah2, Yasuo Takeda3, Shi-Qiang Wang4, Melitta Schachner5,6, Kazutada Watanabe7, Catherine J Pallen8 and Zhi-Cheng Xiao 1,9 1Institute of Molecular and Cell Biology, Singapore 2Singapore 3Department of Clinical Pharmacy and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka, Kagoshima, Japan 4State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking University College of Life Sciences, Beijing, China 5Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA 6Sino-German Center for Neuroscience, Dalian Medical University, Dalian, China 7Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan 8Department of Pediatrics, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada 9Department of Clinical Research, Singapore General Hospital, Singapore *Corresponding author. Department of Clinical Research, Singapore General Hospital, Institute of Molecular and Cell Biology, 61 Biopolis Drive Proteos, Proteos, Singapore 138673, Singapore. Tel.: +65 6326 6195; Fax: +65 6321 3606; E-mail: [email protected] or [email protected] The EMBO Journal (2008)27:188-200https://doi.org/10.1038/sj.emboj.7601939 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Apical dendrites of pyramidal neurons in the neocortex have a stereotypic orientation that is important for neuronal function. Neural recognition molecule Close Homolog of L1 (CHL1) has been shown to regulate oriented growth of apical dendrites in the mouse caudal cortex. Here we show that CHL1 directly associates with NB-3, a member of the F3/contactin family of neural recognition molecules, and enhances its cell surface expression. Similar to CHL1, NB-3 exhibits high-caudal to low-rostral expression in the deep layer neurons of the neocortex. NB-3-deficient mice show abnormal apical dendrite projections of deep layer pyramidal neurons in the visual cortex. Both CHL1 and NB-3 interact with protein tyrosine phosphatase α (PTPα) and regulate its activity. Moreover, deep layer pyramidal neurons of PTPα-deficient mice develop misoriented, even inverted, apical dendrites. We propose a signaling complex in which PTPα mediates CHL1 and NB-3-regulated apical dendrite projection in the developing caudal cortex. Introduction Dendrite development is a fundamental process in the formation of a functional nervous system. Communication between neurons requires proper synaptic formation between axons and dendrites. The extent and pattern of dendritic branching determine the range and scope of synaptic inputs and, to a large extent, the output of a neuron (Jan and Jan, 2003). Compared with our current understanding of axon outgrowth and guidance, the control of dendrite development is much less well understood and has proven to be difficult to tackle. However, some progress has been made in understanding certain aspects of dendrite development, such as the oriented growth of apical dendrites of pyramidal neurons in the neocortex. Pyramidal neurons are a major cell type in the neocortex with a single apical dendrite growing toward the pial surface and several highly branched basal dendrites emanating from the cell body. A diffusible chemoattractant present at high levels near the marginal zone can orient apical dendrites toward the pial surface. Semaphorin 3A (Sema3A), acting through its receptor neuropilin-1, is a good candidate for such a factor (Polleux et al, 2000; Whitford et al, 2002). Genetic and biochemical evidence suggests that p59fyn and Cdk5 mediate Sema3A signaling in the orientation of apical dendrites (Sasaki et al, 2002). Recently, another cell surface molecule, Close Homolog of L1 (CHL1), has been shown to regulate apical dendrite orientation in the neocortex. CHL1 is a type I transmembrane protein that belongs to L1 family of cell adhesion molecules (CAMs), a subclass of neural recognition molecules of the immunoglobulin superfamily. In the developing mouse cortex, CHL1 is expressed in deep layer pyramidal neurons in a low-rostral to high-caudal gradient. Accordingly, in the caudal cortex (somatosensory and visual cortices) of Chl1−/− mice, a significant proportion (40–60%) of deep layer pyramidal neurons exhibit misoriented apical dendrites (Demyanenko et al, 2004). Loss of CHL1 also decreases the rate of radial migration and causes the shift of some neurons to lower laminar positions in the caudal cortex (Demyanenko et al, 2004). Interestingly, CHL1-deficient mice display alterations in emotional reactivity and motor coordination (Montag-Sallaz et al, 2002; Pratte et al, 2003). Mutations in call (the chl1 ortholog in human) have been associated with mental retardation and schizophrenia (Angeloni et al, 1999; Chen et al, 2005). Abnormality in radial migration and apical dendrite development may underlie brain malfunction in mice and humans that carry mutations in the chl1 gene. However, the incomplete loss-of-function phenotype in apical dendrite projections in the Chl1−/− neocortex suggests that other molecules are involved in the same process. The mechanism of CHL1 signaling in apical dendrite projections is not clear either. In search for proteins associated with CHL1, we found that NB-3/contactin-6, a member of the F3/contactin family of neural recognition molecules, exists in a complex with CHL1. The F3/contactin family consists of six structurally related proteins: F3/contactin, TAG-1, BIG-1, BIG-2, NB-2, and NB-3. NB-3 lacks an intracellular domain and is anchored at the cell surface via a glycosylphosphatidylinositol (GPI) link. NB-3 has a similar extracellular domain structure as CHL1, consisting of six Ig-like domains and four fibronectin type III (FNIII) repeats (CHL1 has 4.5 FNIII repeats). Interestingly, genomic mapping reveals that the nb-3 and chl1 genes are closely linked. In the mouse genome, they are located on chromosome 6, only 0.8 Mb apart without any other gene in between. In the human genome, these two genes are located on chromosome 3p26. The distance is 0.5 Mb, with only one hypothetical gene in between. NB-3 is expressed exclusively in the nervous system. NB-3 expression in the mouse cortex is maximal at postnatal day 7 (P7), and declines to a lower level thereafter (Lee et al, 2000). At P7, NB-3 is expressed in a graded pattern in the neocortex, with higher expression levels observed in the caudal cortex (Takeda et al, 2003). These findings suggest a potential role for NB-3 in cortical development and function. However, cortical abnormalities, especially in dendrite development, of Nb-3−/− mice have not been investigated. Early work indicated that L1 family members function not only as adhesion molecules, but are also capable of transducing signals into cells upon activation (Maness and Schachner, 2007). CHL1 has a short cytoplasmic domain that binds ankyrin, a spectrin adapter that couples CHL1 to the subcortical actin cytoskeleton. CHL1 acts as a cooperative partner for integrins in promoting migration (Buhusi et al, 2003; Demyanenko et al, 2004). However, the signal transducers for CHL1 that regulate apical dendrite projection are not clear. Several lines of evidence suggest that protein tyrosine phosphatase α (PTPα), a receptor-like protein phosphatase, may be a potential signal mediator of CHL1 and/or NB-3 in the developing cortical neurons. PTPα engages in cis-interactions with neural cell adhesion molecule (NCAM) and F3/contactin, and mediates their signaling to the intracellular tyrosine kinase p59fyn (Zeng et al, 1999; Bodrikov et al, 2005). The kinase activity of p59fyn is inhibited through intramolecular interaction between phosphorylated Tyr-531 and its SH2 domain, which stabilizes a noncatalytic conformation. PTPα activates p59fyn via dephosphorylation of the Tyr-531 site, and in PTPα−/− mouse brains the phosphorylation of p59fyn at Tyr531 is increased and results in reduced p59fyn activity (Bhandari et al, 1998; Ponniah et al, 1999; Su et al, 1999). PTPα is expressed in deep layers of the developing mouse neocortex and hippocampus. Moreover, radial migration of neurons is impaired in both the hippocampus and neocortex of Ptpα−/− mice (Petrone et al, 2003). Interestingly, inversion of apical dendrites of cortical pyramidal neurons was also observed in mice lacking p59fyn (Sasaki et al, 2002). These findings, taken together, prompted us to investigate possible interaction of PTPα with CHL1 and the role of PTPα in dendrite development in neocortex. Here, we investigated the molecular mechanism of neural recognition molecule-regulated dendrite development of pyramidal neurons in the mouse neocortex. We provide evidence indicating that NB-3 also participates in regulation of apical dendrite development in the caudal cortex. Both CHL1 and NB-3 interact with PTPα and regulate its activity. PTPα is also required for correct apical dendrite projections in the neocortex. These results indicate that PTPα mediates NB-3- and CHL1-regulated apical dendrite development in the deep layer of caudal neocortex. Results CHL1 interacts with NB-3 and enhances its cell surface expression To identify proteins that associate with CHL1, we performed co-immunoprecipitation from mouse brain membrane fractions. CHL1 was detected in the precipitates prepared using a rabbit NB-3 antibody (Figure 1A). In contrast, nonspecific rabbit IgG did not precipitate detectable CHL1 (Figure 1A). This co-immunoprecipitation appeared specific since L1, a family member of CHL1 with high homology, was not precipitated by the same NB-3 antibody (Figure 1A). To confirm the interaction between CHL1 and NB-3, we next performed co-immunoprecipitation from transfected HEK293T cells. Cells expressing either NB-3-Myc or CHL1-HA, or both proteins were immunoprecipitated with an anti-Myc antibody. CHL1-HA was detected in the precipitates from the NB-3-Myc/CHL1-HA-coexpressing cells, but not from mock- or single-transfected cells (Figure 1B). Reciprocal experiments were carried out in which NB-3-Myc was detected in the anti-HA immunoprecipitates from the NB-3-Myc/CHL1-HA-coexpressing cells (Figure 1B). Next, to check if NB-3 directly binds to CHL1, purified NB-3/Fc protein was added to wells coated with purified CHL1/Fc, L1/Fc, or F3/Fc proteins. Binding of NB-3/Fc to the coated wells was detected using the rabbit anti-NB-3 antibody. NB-3/Fc showed strong binding to immobilized CHL1/Fc and F3/Fc, but not to L1/Fc (Figure 1D), indicating that there is direct binding between NB-3 and CHL1. Colocalization of NB-3 and CHL1 was also observed in the soma and neurites of cultured cortical neurons (Figure 1E). Together, these results indicate that NB-3 and CHL1 engage in physical interactions. Figure 1.NB-3 interacts with CHL1. (A) CHL1 was co-immunoprecipitated with NB-3 from mouse brains. P7 mouse brain membrane fractions immunoprecipitated (IP) with either rabbit anti-NB-3 or nonspecific rabbit IgG were probed for the presence of CHL1 or L1 as indicated. Input, lysates before immunoprecipitation; IgG, nonspecific rabbit IgG. (B) Association of NB-3-Myc and CHL1-HA in HEK293T cells. Transfected HEK293T cells were precipitated with anti-Myc or anti-HA antibodies and were probed with anti-HA or anti-Myc antibodies, respectively. (C) Purity of recombinant mouse NB-3/Fc and CHL1/Fc proteins used in ELISA as determined by SDS–PAGE and Coomassie blue stain. (D) Binding of NB-3/Fc (50 μg/ml) to immobilized CHL1/Fc (▪), L1/Fc (▴), and F3/Fc (•) (100 μg/ml) in ELISA plates. Background was estimated in BSA-coated (1 mg/ml) wells. Readings for wells coated with NB-3/Fc (5 μg/ml) followed by ELISA were given the value as 1.0. Relative binding values for each well were calculated accordingly. Values are from two independent experiments performed in triplicate. (E) Colocalization of endogenous NB-3 and CHL1 on soma and neurites of cultured cortical neurons. E17 cortical culture (5–10 DIV) were fixed and stained with rabbit anti-NB-3 and mouse anti-CHL1 antibodies. Scale bars, 30 μm. Download figure Download PowerPoint Neural recognition molecules are clustered and activated when binding to their ligands (Bodrikov et al, 2005). To check whether NB-3 and CHL1 could engage in a trans-interaction, we added purified NB-3/Fc protein to live cortical neurons. Unlike the antibody against CHL1, NB-3/Fc did not induce clustering of the cell surface CHL1; neither did CHL1/Fc protein change the surface distribution of NB-3 (Supplementary Figure 1). Moreover, NB-3/Fc-coated microspheres did not bind to CHL1-expressing COS1a cells (data not shown). These results suggest that NB-3 and CHL1 do not function as ligand for each other, and they may engage in a cis-interaction. In COS1a cells expressing only NB-3-Myc, NB-3-Myc protein was mostly located inside the cells (Figure 2A), and only a very faint cell surface staining for NB-3-Myc was detected (Figure 2B(a)). Interestingly, when coexpressed with CHL1-HA, which was mainly located on the cell surface, some NB-3-Myc proteins moved to the cell periphery and colocalized with cell surface CHL1-HA (Figure 2A). We also observed a strong cell surface staining for NB-3-Myc in these cells (Figure 2B(b–e)). Notably, CHL1-negative cells, although in contact with CHL1-positive cells, had a low level of surface NB-3-Myc (Figure 2B(c–e), arrowheads). Figure 2.CHL1 enhances cell surface expression of NB-3. (A) Transfected COS1a cells were fixed and stained with anti-Myc and/or anti-HA antibodies to reveal the localization of NB-3-Myc and CHL1-HA proteins. When expressed alone, NB-3-Myc was mainly located inside the cells. When coexpressed with CHL1-HA, some NB-3-Myc protein moved to the cell periphery and colocalized with CHL1-HA (arrowheads). Scale bars, 50 μm. (B) Increase of cell surface NB-3-Myc when coexpressed with CHL1-HA in COS1a cells. After transfected with NB-3-Myc alone (a) or with both NB-3-Myc and CHL1-HA cDNAs (b–e), live COS1a cells were incubated with anti-Myc antibody followed by fixation and staining for cell surface NB-3-Myc protein alone (a and b), or double staining with anti-HA for CHL1-HA proteins (c–e). Note the cells with high surface NB-3-Myc level also expressed high level of CHL1-HA; CHL1-negative cells (arrowheads) had low levels of surface NB-3-Myc. Scale bars, 50 μm. (C) Analysis of surface expression of NB-3-Myc and CHL1-HA in single- and double-transfected COS1a cells. Cell surface proteins were biotinylated and precipitated using NeutrAvidin Gel. Pull-down samples were blotted with anti-Myc or anti-HA antibodies to assess the level of cell surface NB-3-Myc and CHL1-HA proteins. (D, E) Analysis of surface expression of NB-3 protein in cortical neurons from Chl1−/− mice (D) and CHL1 protein in neurons from Nb-3−/− mice (E). Cell surface proteins of E17 cortical cultures (7 DIV) were biotinylated. Pull-down samples using NeutrAvidin Gel were blotted with antibodies as indicated. For quantification in panels C–E, levels of cell surface proteins were normalized to corresponding input proteins. Results from four independent experiments (n=4) are presented as mean±s.e.m. for each panel. *P<0.05; one-sample t-test. α-α-Tub, anti-α-tubulin antibody. Download figure Download PowerPoint To quantitatively analyze surface expression of NB-3-Myc in transfected COS1a cells, cell surface proteins were biotinylated and precipitated using a NeutrAvidin™ Gel. Probing of precipitates with anti-Myc antibody revealed the amount of cell surface NB-3-Myc. We found an average of 3.7±0.8-fold increase in the amount of cell surface NB-3-Myc in cotransfected cells, compared with that of the NB-3-Myc single-transfected cells, while the cell surface CHL1-HA level was not affected by the coexpressing of NB-3-Myc (Figure 2C). The increase in cell surface NB-3-Myc appeared to be a specific result of coexpression with CHL1-HA, as coexpression with L1 did not significantly change the surface expression of NB-3-Myc in transfected COS1a cells (Supplementary Figure 2). NB-3 and CHL1 are normally expressed in neurons that are very different from transfected COS1a cells. To assess whether CHL1 also affects surface expression of NB-3 in neurons, the surface level of endogenous NB-3 was analyzed in cultured cortical neurons from wild-type and Chl1−/− mice. We detected a significant reduction of cell surface NB-3 in the Chl1−/− cortical neurons (62.1±9.4% of the wild type, Figure 2D). In the Nb-3−/− cortical neurons, however, the level of cell surface CHL1 protein was similar to the wild-type neurons (111.7±13.5% of the wild type, Figure 2E). Together, these results indicate that CHL1 interacts with NB-3 and positively regulates its surface expression in neurons, while NB-3 has no effect on the surface expression of CHL1. Graded expression of NB-3 in developing cortical pyramidal neurons To assess the potential role of NB-3 in cortical development, the spatiotemporal expression of NB-3 was analyzed in the developing mouse neocortex. X-gal staining of the Nb-3+/− mouse, which has a LacZ gene inserted into the Nb-3 locus, could reflect the normal NB-3 expression pattern (Takeda et al, 2003). A weak X-gal signal in the intermediate zone of neocortex was detected as early as embryonic day 15.5 (E15.5) (data not shown). At around E17, deep layer pyramidal neurons in the neocortex begin to extend axonal and dendritic processes. At this stage, X-gal signal was observed in the deeper layer of the caudal cortex (Figure 3A(a and b)). At postnatal day 0 (P0), X-gal signal was mainly present in the layer V of the caudal cortex (Figure 3A(c)), and was higher in the visual cortex than in the auditory cortex (Figure 3A(d)). High-caudal to low-rostral pattern of X-gal signal in the cortex was maintained at P7 (Figure 3A(e and f)) (Takeda et al, 2003) and as late as P17 (data not shown). Figure 3.Expression of NB-3 in the developing mouse neocortex. (A) Localization of cells expressing NB-3 monitored by LacZ expression in the medial sagittal (a, c, and e) and coronal (d and f) sections of the Nb-3+/− brains at various developmental stages. (b) Higher magnification of the selected area in (a). Scale bars, 1 mm. (B) Immunostaining of the medial sagittal sections of P7 wild-type mouse neocortex using rabbit anti-NB-3 antibody. Arrowheads point to NB-3-positive cells. (d–f) Higher magnification of the selected areas in (a–c), respectively. (g) Control staining of Nb-3−/− layer V visual cortex (P7) with the same NB-3 antibody. Motor, motor cortex; SS, somatosensory cortex; Visual, visual cortex. Scale bars, 100 μm. (C) E17 cortical culture (a–f) or P7 mouse brain (g–i) were stained with rabbit anti-NB-3 antibody and monoclonal antibodies against MAP2 or GFAP. NB-3 is expressed in dendrites and soma of neurons in cortical culture (a–c) and brain tissues (g–i; arrowheads points to proximal apical dendrites) but not in astrocytes (d–f). Scale bars, 25 μm. (D) Specificity of the rabbit anti-NB-3 antibody. Whole brain homogenates from wild-type and Nb-3−/− mice were resolved by SDS–PAGE, followed by immunoblotting with the anti-NB-3 antibody. Download figure Download PowerPoint Immunofluorescent staining of NB-3 protein in the wild-type mouse cortex (P7) also showed the strongest signal in the visual cortex, with many deep layer pyramidal neurons stained (Figure 3B(c and f)). Staining in layer I–II may be due to NB-3 protein present in the apical dendritic tufts of deep layer neurons, although the signal was also present on some cell bodies. A moderate number of layer V cells were stained in the somatosensory cortex (Figure 3B(b and e)), and even less in the motor cortex (Figure 3B(a and d)). Staining of the Nb-3−/− cortex with the same NB-3 antibody was minimal (P7 littermate; Figure 3B(g)). The pattern of NB-3 protein expression was largely consistent with the X-gal signal in the Nb-3+/− cortex. Together, these results indicate that NB-3 is expressed in a low-rostral to high-caudal gradient, with the highest level in the visual cortex during the time when deep layer pyramidal neurons develop axonal and dendritic processes. In cultured mouse cortical neurons, only a small percentage of cells (<10%) exhibited higher than background signals when stained with the NB-3 antibody, consistent with the fact that NB-3 is predominantly expressed in layer V of the caudal cortex. NB-3 was expressed in the soma and processes of cortical neurons, including dendrites (Figure 3C(a–c)) and axons (data not shown). Little NB-3 signal was observed in astrocytes (Figure 3C(d–f)). Double staining of P7 visual cortex for NB-3 and MAP2 also showed NB-3 expression in the dendrites of layer V pyramidal neurons (Figure 3C(g–i)). Misorientation of apical dendrites in layer V pyramidal neurons of the Nb-3−/− mouse neocortex The physical interaction between NB-3 and CHL1 and their similar expression pattern suggested that NB-3 functions together with CHL1 in the cortical development. We therefore investigated the positioning and morphology of pyramidal neurons in the Nb-3−/− cortex. The organization of the Nb-3−/− neocortex was indistinguishable from that of the wild-type littermates, with all layers present in the same order (Takeda et al, 2003). To investigate if Nb-3−/− mice exhibit altered layer V pyramidal neuronal distribution as observed in the Chl1−/− mice, NB-3 mutant mice were crossed with a reporter strain in which layer V pyramidal neurons are intrinsically labeled with enhanced yellow fluorescent protein (YFP) (Thy1/YFP transgenic mice line H) (Feng et al, 2000; Demyanenko et al, 2004). In all cortical areas examined, the distribution of layer V pyramidal neurons in the Nb-3−/− mice was indistinguishable from that of the wild-type littermates (Figure 4A and Supplementary Figure 3), suggesting that, unlike CHL1, NB-3 does not regulate migration of deep layer pyramidal neurons. Figure 4.Misorientation of apical dendrites of layer V pyramidal neurons in the visual cortex of Nb-3−/− mice. (A) Morphology of layer V pyramidal neurons in the motor, somatosensory (SS), visual and auditory cortices of Nb-3+/+ (a–d, i) and Nb-3−/− (e–h, j) littermates. To visualize the morphology of layer V neurons in neocortex, NB-3 mutant mice were crossed to the Thy1-YFPH transgenic mice, which express YFP in layer V cells in the neocortex. (i) and (j) are higher magnification of the selected areas in (c) and (g), respectively. Arrowheads point to neurons displaying misoriented apical dendrites. Scale bar, 100 μm. (B) Golgi-impregnated visual cortex of wild-type (wt) and Nb-3−/− mice (1-month-old littermates). Arrows point to neurons with misoriented apical dendrites. Scale bars, 30 μm. (C) Scoring method for apical dendrite orientation (see Supplementary data). (D, E) Average ∣θ∣ of layer III (D) and layer V (E) pyramidal neurons of motor, somatosensory, visual and auditory cortices from wild-type and Nb-3−/− littermates. (F, G) Proportion of pyramidal neurons with misoriented apical dendrites was significantly increased in layer V but not in the layer III visual cortex of Nb-3−/− mice. Misoriented apical dendrites were defined as those with ∣θ∣>8°. For the above quantification, three to six pairs of wild-type and Nb-3−/− littermates (1-month-old) were analyzed. The number for layer V neurons ranged from 80 to 445 for each cortical region of respective genotypes, and ranged from 40 to 145 for layer III neurons. Results are presented as mean±s.e.m. ***P<0.001; two-way ANOVA with repeated measures followed by Bonferroni post-tests. Download figure Download PowerPoint However, in the Nb-3−/− visual cortex, we observed abnormal apical dendrites oriented sideways in many layer V pyramidal neurons, although they ultimately reached layer I and formed apical tufts (Figure 4A(g and j)). Golgi impregnation of Nb-3−/− brains also revealed misoriented apical dendrites in layer V pyramidal neurons of the visual cortex (Figure 4B). This abnormality was not observed in the motor, somatosensory, and auditory cortices (Figure 4A), or in the upper layer of the visual cortex (Figure 4B). To quantitatively compare the apical dendrite orientation of pyramidal neurons in the Nb-3−/− and wild-type cortices (1-month-old), Golgi impregnation was carried out and the direction of apical dendrites was measured as an angle of orientation relative to the pial surface (θ) (Figure 4C). Among all the cortical areas examined, only in the layer V visual cortex was the average ∣θ∣ significantly increased in the Nb-3−/− mice (17.1±1.3°) compared with the wild-type littermates (4.7±0.2°; Figure 4E). For the upper layer pyramidal neurons, the average ∣θ∣ in the Nb-3−/− mice was not significant different from that of the wild-type littermates in all cortical areas (Figure 4D). In each area of the wild-type cortex, 80–90% of neurons had apical dendrites with ∣θ∣⩽8°. Neurons with apical dendrites that projected outside this normal range (∣θ∣>8°) were significantly increased (60.4±2.5%) in layer V of Nb-3−/− visual cortex compared with those in the wild-type littermates (12.2±2.0%) (Figure 4G). This increase was not observed in layer V of other cortical areas (Figure 4G), or in layer III of all cortical regions including the visual cortex (Figure 4F). However, unlike Chl1−/− mice, the number of layer V pyramidal neurons with inverted dendrites (∣θ∣>90°) was not significantly increased in the Nb-3−/− visual cortex (data not shown). Thus, Nb-3−/− mice exhibit a similar, yet less severe, loss-of-function phenotype in apical dendrite projections in the caudal cortex as compared to Chl1−/− mice. Owing to the close linkage of nb-3 and chl1 genes on the chromosome, there is a possibility that disruption of one gene changes the expression level of the other, resulting in the similar phenotypes observed in these two types of knockout mice. However, we found that in the Nb-3−/− brains, the total CHL1 protein level was similar to the wild-type littermates, and vice versa (Supplementary Figure 4), suggesting that the defects observed in the Nb-3−/− mice were not due to changes in CHL1 expression level in the cortex of the mutant mice. The interaction of" @default.
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• W2022889556 title "Neural recognition molecules CHL1 and NB-3 regulate apical dendrite orientation in the neocortex via PTPα" @default.
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