FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022891261> ?p ?o ?g. }

• W2022891261 endingPage "51" @default.
• W2022891261 startingPage "47" @default.
• W2022891261 abstract "In this study, a cDNA clone coding for sorghum leaf NADP-malate dehydrogenase [Crétin, C., Luchetta, P., Joly, C., Decottignies, P., Lepiniec, L., Gadal, P., Sallantin, M., Huet, J. C. & Pernollet, J. C. (1990) Eur. J. Biochem. 192, 299–303] was used either in the full-length form or in a shorter form deprived of the 5′ end coding for the transit peptide. Both cDNA fragments were cloned into the expression vector pKK233-2 and the resulting constructions were used to transform E. coli cells. The bacterial cells which do not contain any NADP-dependent malate dehydrogenase before transformation were able to express the protein after transformation and induction, as detected both by activity measurements and by immunoblot. The recombinant proteins could be purified to homogeneity and their biochemical characteristics studied. They were identical to those of the enzyme isolated from corn or sorghum leaves, including the well known redox regulatory properties. The NADP–malate dehydrogenases derived from both constructions had a similar subunit size and the analysis of their N-terminal sequences revealed that E. coli cells were able to recognize the processing signal of the precursor polypeptide and to mature and assemble the protein in a manner similar to higher plants." @default.
• W2022891261 created "2016-06-24" @default.
• W2022891261 creator A5013665346 @default.
• W2022891261 creator A5018264642 @default.
• W2022891261 creator A5030152534 @default.
• W2022891261 creator A5043106379 @default.
• W2022891261 creator A5052124306 @default.
• W2022891261 creator A5071534674 @default.
• W2022891261 creator A5078839593 @default.
• W2022891261 date "1991-07-01" @default.
• W2022891261 modified "2023-09-26" @default.
• W2022891261 title "Properties of recombinant NADP-malate dehydrogenases from Sorghum vulgare leaves expressed in Escherichia coli cells" @default.
• W2022891261 cites W1426433934 @default.
• W2022891261 cites W1521406343 @default.
• W2022891261 cites W1540246217 @default.
• W2022891261 cites W1794227179 @default.
• W2022891261 cites W1803924755 @default.
• W2022891261 cites W1964291174 @default.
• W2022891261 cites W1966650078 @default.
• W2022891261 cites W1973528267 @default.
• W2022891261 cites W1973846400 @default.
• W2022891261 cites W1973968888 @default.
• W2022891261 cites W1977036272 @default.
• W2022891261 cites W1979171257 @default.
• W2022891261 cites W1987616853 @default.
• W2022891261 cites W2004480077 @default.
• W2022891261 cites W2017168119 @default.
• W2022891261 cites W2028622989 @default.
• W2022891261 cites W2029987769 @default.
• W2022891261 cites W2035663056 @default.
• W2022891261 cites W2049105193 @default.
• W2022891261 cites W2049547477 @default.
• W2022891261 cites W2052533514 @default.
• W2022891261 cites W2056354282 @default.
• W2022891261 cites W2068934297 @default.
• W2022891261 cites W2072059333 @default.
• W2022891261 cites W2072588674 @default.
• W2022891261 cites W2077893267 @default.
• W2022891261 cites W2100837269 @default.
• W2022891261 cites W2101108802 @default.
• W2022891261 cites W2135849317 @default.
• W2022891261 doi "https://doi.org/10.1111/j.1432-1033.1991.tb16090.x" @default.
• W2022891261 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2065679" @default.
• W2022891261 hasPublicationYear "1991" @default.
• W2022891261 type Work @default.
• W2022891261 sameAs 2022891261 @default.
• W2022891261 citedByCount "22" @default.
• W2022891261 countsByYear W20228912612014 @default.
• W2022891261 countsByYear W20228912612021 @default.
• W2022891261 crossrefType "journal-article" @default.
• W2022891261 hasAuthorship W2022891261A5013665346 @default.
• W2022891261 hasAuthorship W2022891261A5018264642 @default.
• W2022891261 hasAuthorship W2022891261A5030152534 @default.
• W2022891261 hasAuthorship W2022891261A5043106379 @default.
• W2022891261 hasAuthorship W2022891261A5052124306 @default.
• W2022891261 hasAuthorship W2022891261A5071534674 @default.
• W2022891261 hasAuthorship W2022891261A5078839593 @default.
• W2022891261 hasBestOaLocation W20228912611 @default.
• W2022891261 hasConcept C104317684 @default.
• W2022891261 hasConcept C132901563 @default.
• W2022891261 hasConcept C153911025 @default.
• W2022891261 hasConcept C181199279 @default.
• W2022891261 hasConcept C187882448 @default.
• W2022891261 hasConcept C204241405 @default.
• W2022891261 hasConcept C2776317432 @default.
• W2022891261 hasConcept C40767141 @default.
• W2022891261 hasConcept C547475151 @default.
• W2022891261 hasConcept C55493867 @default.
• W2022891261 hasConcept C86803240 @default.
• W2022891261 hasConceptScore W2022891261C104317684 @default.
• W2022891261 hasConceptScore W2022891261C132901563 @default.
• W2022891261 hasConceptScore W2022891261C153911025 @default.
• W2022891261 hasConceptScore W2022891261C181199279 @default.
• W2022891261 hasConceptScore W2022891261C187882448 @default.
• W2022891261 hasConceptScore W2022891261C204241405 @default.
• W2022891261 hasConceptScore W2022891261C2776317432 @default.
• W2022891261 hasConceptScore W2022891261C40767141 @default.
• W2022891261 hasConceptScore W2022891261C547475151 @default.
• W2022891261 hasConceptScore W2022891261C55493867 @default.
• W2022891261 hasConceptScore W2022891261C86803240 @default.
• W2022891261 hasIssue "1" @default.
• W2022891261 hasLocation W20228912611 @default.
• W2022891261 hasLocation W20228912612 @default.
• W2022891261 hasOpenAccess W2022891261 @default.
• W2022891261 hasPrimaryLocation W20228912611 @default.
• W2022891261 hasRelatedWork W1988346978 @default.
• W2022891261 hasRelatedWork W2038831974 @default.
• W2022891261 hasRelatedWork W2061338339 @default.
• W2022891261 hasRelatedWork W2170874906 @default.
• W2022891261 hasRelatedWork W2338137066 @default.
• W2022891261 hasRelatedWork W2350146650 @default.
• W2022891261 hasRelatedWork W2373690171 @default.
• W2022891261 hasRelatedWork W2382285667 @default.
• W2022891261 hasRelatedWork W2386419125 @default.
• W2022891261 hasRelatedWork W349977252 @default.
• W2022891261 hasVolume "199" @default.
• W2022891261 isParatext "false" @default.
• W2022891261 isRetracted "false" @default.

• next