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- W2022892421 abstract "Metabolic engineering represents a promising approach to enhance the yield of valuable natural products from plants. A method to quantify flux through metabolite measurements is necessary for the analysis of native and modified pathways. Rather than focusing only on the accumulation of the final products, analyzing a wide range of secondary metabolites has significant advantages. We propose a model that organizes the flux analysis by grouping metabolites of similar biosynthetic origin. To this end, we have quantified temporal profiles of metabolites from several branches of the indole alkaloid pathway in Catharanthus roseus hairy root cultures. By analyzing these data, we are able to examine the distribution of flux around key branchpoints. Furthermore, this analysis provides crucial information such as an estimate of total flux to secondary metabolism." @default.
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- W2022892421 date "2002-07-01" @default.
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- W2022892421 title "Quantification of Metabolic Flux in Plant Secondary Metabolism by a Biogenetic Organizational Approach" @default.
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- W2022892421 doi "https://doi.org/10.1006/mben.2002.0224" @default.
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