Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022893370> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2022893370 endingPage "31" @default.
- W2022893370 startingPage "25" @default.
- W2022893370 abstract "The toxicity of the suicide HSVtk gene approach is known to be targeted to DNA synthesis and, consequently, to dividing cells. This system is therefore useful for the treatment of brain tumors which contain dividing cells surrounded by a quiescent normal tissue. Adenoviruses are efficient vectors for the transfer of the HSVtk gene into the tumor but this can lead to the transduction of quiescent cells. In this study, we focused on the toxicity of the HSVtk/ganciclovir treatment for the two main cell types of the normal brain: astrocytes and neurons. Astrocytes and neurons in primary culture were infected by an adenoviral vector bearing the HSVtk gene (Ad.tk) and cells were exposed to different concentrations of ganciclovir. After 5 days of treatment, an MTT test measured a dramatic decrease in cell viability for treated astrocytes while a small decrease in cell viability was observed for neurons treated in the same experimental conditions. The differential toxicity of the HSVtk/ganciclovir treatment was also observed in cocultures of astrocytes and neurons: an immunocytochemical analysis of the treated cells showed major morphological modifications for astrocytes but not for neurons. Furthermore, our data suggest that a bystander effect is able to kill all the astrocytes while neurons from the same culture remain unaffected." @default.
- W2022893370 created "2016-06-24" @default.
- W2022893370 creator A5013260560 @default.
- W2022893370 creator A5015188143 @default.
- W2022893370 creator A5026379062 @default.
- W2022893370 creator A5036433683 @default.
- W2022893370 creator A5068749565 @default.
- W2022893370 creator A5070477307 @default.
- W2022893370 date "1997-01-01" @default.
- W2022893370 modified "2023-10-14" @default.
- W2022893370 title "Differential toxicity of ganciclovir for rat neurons and astrocytes in primary culture following adenovirus-mediated transfer of the HSVtk gene" @default.
- W2022893370 doi "https://doi.org/10.1038/sj.gt.3300353" @default.
- W2022893370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9068792" @default.
- W2022893370 hasPublicationYear "1997" @default.
- W2022893370 type Work @default.
- W2022893370 sameAs 2022893370 @default.
- W2022893370 citedByCount "14" @default.
- W2022893370 countsByYear W20228933702016 @default.
- W2022893370 crossrefType "journal-article" @default.
- W2022893370 hasAuthorship W2022893370A5013260560 @default.
- W2022893370 hasAuthorship W2022893370A5015188143 @default.
- W2022893370 hasAuthorship W2022893370A5026379062 @default.
- W2022893370 hasAuthorship W2022893370A5036433683 @default.
- W2022893370 hasAuthorship W2022893370A5068749565 @default.
- W2022893370 hasAuthorship W2022893370A5070477307 @default.
- W2022893370 hasBestOaLocation W20228933701 @default.
- W2022893370 hasConcept C104317684 @default.
- W2022893370 hasConcept C111599444 @default.
- W2022893370 hasConcept C126322002 @default.
- W2022893370 hasConcept C15152581 @default.
- W2022893370 hasConcept C153911025 @default.
- W2022893370 hasConcept C19106626 @default.
- W2022893370 hasConcept C203014093 @default.
- W2022893370 hasConcept C2777965375 @default.
- W2022893370 hasConcept C2779820661 @default.
- W2022893370 hasConcept C29730261 @default.
- W2022893370 hasConcept C32470452 @default.
- W2022893370 hasConcept C34981463 @default.
- W2022893370 hasConcept C40767141 @default.
- W2022893370 hasConcept C53227056 @default.
- W2022893370 hasConcept C54355233 @default.
- W2022893370 hasConcept C55493867 @default.
- W2022893370 hasConcept C71924100 @default.
- W2022893370 hasConcept C81885089 @default.
- W2022893370 hasConcept C86803240 @default.
- W2022893370 hasConcept C95444343 @default.
- W2022893370 hasConceptScore W2022893370C104317684 @default.
- W2022893370 hasConceptScore W2022893370C111599444 @default.
- W2022893370 hasConceptScore W2022893370C126322002 @default.
- W2022893370 hasConceptScore W2022893370C15152581 @default.
- W2022893370 hasConceptScore W2022893370C153911025 @default.
- W2022893370 hasConceptScore W2022893370C19106626 @default.
- W2022893370 hasConceptScore W2022893370C203014093 @default.
- W2022893370 hasConceptScore W2022893370C2777965375 @default.
- W2022893370 hasConceptScore W2022893370C2779820661 @default.
- W2022893370 hasConceptScore W2022893370C29730261 @default.
- W2022893370 hasConceptScore W2022893370C32470452 @default.
- W2022893370 hasConceptScore W2022893370C34981463 @default.
- W2022893370 hasConceptScore W2022893370C40767141 @default.
- W2022893370 hasConceptScore W2022893370C53227056 @default.
- W2022893370 hasConceptScore W2022893370C54355233 @default.
- W2022893370 hasConceptScore W2022893370C55493867 @default.
- W2022893370 hasConceptScore W2022893370C71924100 @default.
- W2022893370 hasConceptScore W2022893370C81885089 @default.
- W2022893370 hasConceptScore W2022893370C86803240 @default.
- W2022893370 hasConceptScore W2022893370C95444343 @default.
- W2022893370 hasIssue "1" @default.
- W2022893370 hasLocation W20228933701 @default.
- W2022893370 hasLocation W20228933702 @default.
- W2022893370 hasOpenAccess W2022893370 @default.
- W2022893370 hasPrimaryLocation W20228933701 @default.
- W2022893370 hasRelatedWork W2004332616 @default.
- W2022893370 hasRelatedWork W2011486273 @default.
- W2022893370 hasRelatedWork W2012315955 @default.
- W2022893370 hasRelatedWork W2055825871 @default.
- W2022893370 hasRelatedWork W2062023486 @default.
- W2022893370 hasRelatedWork W2065434365 @default.
- W2022893370 hasRelatedWork W2079360241 @default.
- W2022893370 hasRelatedWork W2323062703 @default.
- W2022893370 hasRelatedWork W2375455239 @default.
- W2022893370 hasRelatedWork W4249791004 @default.
- W2022893370 hasVolume "4" @default.
- W2022893370 isParatext "false" @default.
- W2022893370 isRetracted "false" @default.
- W2022893370 magId "2022893370" @default.
- W2022893370 workType "article" @default.