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- W2022903908 abstract "An updated method for obtaining purified glucosylgalactosyl hydroxylysine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration. Identification is confirmed by consecutive mild acid hydrolysis of the extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxylysine (Hyl). High-performance liquid chromatography (HPCL) with a sodium acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc-Gal-Hyl is not commercially available but this easy and efficient method can provide large amounts of it for collagen metabolism studies." @default.
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- W2022903908 date "1996-05-01" @default.
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- W2022903908 title "Improved rapid method for the isolation, purification and identification of collagen glycosides" @default.
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- W2022903908 doi "https://doi.org/10.1016/0021-9673(95)01296-6" @default.
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