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- W2022904043 abstract "Many therapeutic strategies have been proposed to treat liver fibrosis, but no drugs have been proved effective. Matrix metalloproteinases (MMPs) have been reported to play a role in some cellular cascades of hepatic inflammation and fibrosis.The purpose of this study was to investigate whether silymarin and pentoxifylline (PTX) have hepatoprotective and antifibrotic effects in experimental hepatic fibrosis.Sprague-Dawley rats were divided into 4 groups: silymarin group (silymarin 4 mg/kg · d(-1) orally, common bile duct ligation [CBDL]); PTX group (PTX 2 mg/kg · d(-1) intraperitoneally, CBDL); sham group (common bile duct [CBD] exploration only); and control group (saline 1 mL/d orally, CBDL). The CBD was explored and dissected sufficiently to allow passage of a 3/0 silk suture via midline laparotomy. On day 10, all animals were euthanized via cervical dislocation. Then, 5-cm(3) liver samples from the right lobe were removed for histomorphologic evaluation and 3-mL blood samples were taken via cardiac puncture for biochemical analyses. Apoptosis was determined using the terminal deoxynucleotidyltransferase-biotin nick end-label (TUNEL) staining method. Plasma levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and γ-glutamyltransferase; total and indirect bilirubin concentration; hepatic MMP-1 and -2 and tissue inhibitor of MMP (TIMP)-l and -2 activity; and transforming-growth factor (TGF)-β1 concentration were measured. Collagen content was determined by measuring hydroxyproline in liver samples. Malondialdehyde (MDA) was used to estimate lipid peroxidation.Thirty-two adult male Sprague-Dawley rats were divided into 4 groups: silymarin group (n = 7), PTX group (n = 7), sham group (n = 9), and control group (n = 9). Compared with the control group (14.6 [2.44]), mean (SD) hepatocyte apoptosis (as measured by the ratio of TUNEL-positive cells) was significantly suppressed in the silymarin group (1.2 [0.13]; P = 0.001) and the PTX group (3.8 [0.34]; P = 0.001). Mean (SD) MMP-2 activity in the silymarin group (57.35 [9.89] μg/mL; P = 0.04) and the PTX group (46.88 [9.56] μg/mL; P = 0.04) was significantly lower than that observed in the control group (232.32 [79.76] μg/mL). Compared with the control group (1.37 [0.38] μg/mL), TIMP-2 activity was significantly lower in the silymarin group (0.55 [0.13] μg/mL; P = 0.04) and the PTX group (0.42 [0.09] μg/mL; P = 0.01). Compared with the control group (909.17 [117.35] μg/mL), TGF-β1 was significantly lower in the silymarin group (518.24 [30.34] μg/mL; P = 0.01) and the PTX group (519.57 [47.27] μg/mL; P = 0.01). Histomorphologic changes were significantly greater in the sham group than in the silymarin and PTX groups: hemorrhage (2.44 [0.29] vs 1.29 [0.18] and 1.57 [0.20], respectively; both, P = 0.04); sinusoidal dilatation (2.22 [0.22] vs 1.57 [0.20] and 1.71 [0.18]; both, P = 0.04); presinusoidal polymorphonuclear cell infiltration (3-44 [0.24] vs 2.57 [0.20] and 2.14 [0.26]; P = 0.03 and P = 0.008, respectively); and inflammation (3.44 [0.24] vs 2.57 [0.20] and 2.14 [0.26]; P = 0.03 and P = 0.008, respectively). In the control group, all biochemical markers were elevated, supporting the presence of liver injury. Compared with the control group (630.00 [46.80] U/L), plasma AST activity was significantly lower in the silymarin group (443.11 [78.73]; P = 0.04) and the PTX group (349.42 [34.00]; P = 0.03). Compared with the control group (191.12 [32.93] U/L), plasma ALT activity was significantly lower in the silymarin group (86.14 [4.97]; P = 0.04) and the PTX group (84.14 [11.21]; P = 0.04). MDA concentration was significantly lower in the silymarin group compared with the control group (0.08 [0.01] vs 0.22 [0.03] nmol/mL; P = 0.004); MDA was also significantly lower in the silymarin group than in the PTX group (0.11 [0.02]; P = 0.03).Silymarin and PTX were associated with lower histopathologic liver damage, hepatocyte apoptosis, and regulation of extracellular matrix proteins. Lipid peroxidation in hepatocytes was significantly lower in the silymarin group compared with the PTX group. Silymarin and PTX appeared to have hepatoprotective effects in this experimental liver fibrosis model, but further clinical and experimental studies are needed." @default.
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- W2022904043 title "Effects of silymarin and pentoxifylline on matrix metalloproteinase-1 and -2 expression and apoptosis in experimental hepatic fibrosis" @default.
- W2022904043 cites W1499850422 @default.
- W2022904043 cites W1600831053 @default.
- W2022904043 cites W1901445830 @default.
- W2022904043 cites W1973774141 @default.
- W2022904043 cites W1983310681 @default.
- W2022904043 cites W1984870498 @default.
- W2022904043 cites W1986527765 @default.
- W2022904043 cites W1989539175 @default.
- W2022904043 cites W1990933733 @default.
- W2022904043 cites W1991382261 @default.
- W2022904043 cites W1992006494 @default.
- W2022904043 cites W1992195166 @default.
- W2022904043 cites W1996361792 @default.
- W2022904043 cites W1996677487 @default.
- W2022904043 cites W2004317356 @default.
- W2022904043 cites W2007551138 @default.
- W2022904043 cites W2008316203 @default.
- W2022904043 cites W2023021687 @default.
- W2022904043 cites W2030019649 @default.
- W2022904043 cites W2033834764 @default.
- W2022904043 cites W2047393168 @default.
- W2022904043 cites W2066504688 @default.
- W2022904043 cites W2072443750 @default.
- W2022904043 cites W2072454412 @default.
- W2022904043 cites W2072591613 @default.
- W2022904043 cites W2076708571 @default.
- W2022904043 cites W2080325240 @default.
- W2022904043 cites W2085071775 @default.
- W2022904043 cites W2086434450 @default.
- W2022904043 cites W2092639174 @default.
- W2022904043 cites W2093554311 @default.
- W2022904043 cites W2095347599 @default.
- W2022904043 cites W2137128482 @default.
- W2022904043 cites W2155405391 @default.
- W2022904043 cites W2160122983 @default.
- W2022904043 cites W2163185124 @default.
- W2022904043 cites W2163637735 @default.
- W2022904043 cites W2171806063 @default.
- W2022904043 cites W2604337567 @default.
- W2022904043 cites W4233778145 @default.
- W2022904043 cites W4255712601 @default.
- W2022904043 doi "https://doi.org/10.1016/j.curtheres.2008.12.003" @default.
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