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• W2022906051 endingPage "493" @default.
• W2022906051 startingPage "488" @default.
• W2022906051 abstract "RNA polymerase was purified from vegetative-phase mycelia of Streptomyces griseus by a series of ion-exchange chromatographies. By western blot analysis using antiserum against S. coelicolor HrdB, which is a principal sigma factor (óhrdB), the purified holoenzyme was found to contain óB (= óhrdB) of S. griseus. Significant amounts of HrdB protein were, however, eluted from the DEAE column at lower concentrations of KCl than that required for elution of the holoenzyme containing óH, suggesting that óB is dissociated from the core enzyme, or an excess amount of óB exists in S. griseus cells. The holoenzyme containing óB (Eón) transcribed in vitro the dagA promoter of S. coelicolor, and the hrdB and hsp70 promoters of 5. griseus, suggesting that it is involved in transcription of the essential genes. EóB may be a major form of RNA polymerase holoenzyme in the growing phase of S. griseus." @default.
• W2022906051 created "2016-06-24" @default.
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• W2022906051 date "1995-09-01" @default.
• W2022906051 modified "2023-10-03" @default.
• W2022906051 title "Purification and Characterization of RNA Polymerase Holoenzyme (EóB) from Vegetative-Phase Mycelia of Streptomyces griseus" @default.
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• W2022906051 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a124934" @default.
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