Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022946064> ?p ?o ?g. }
- W2022946064 endingPage "741" @default.
- W2022946064 startingPage "736" @default.
- W2022946064 abstract "Eukaryotic yeast-based DNA damage cellular sensors offer many advantages to traditional prokaryotic-based mutagenicity assays. The HUG1P-GFP promoter-reporter construct has proven to be an effective method to selectively screen for multiple types of DNA damage. To enhance the sensitivity and selectivity of the system to different types of DNA damage, two genes involved in distinct DNA damage responses were deleted. Deletion of MAG1, a gene encoding a DNA glycosylase and member of the base excision repair (BER) pathway, increased the biosensor's sensitivity to the alkylating agents methyl methanesulfonate (MMS) (lowering the sensitivity threshold to 0.0001% (v/v)) and ethyl methanesulfonate (EMS). Deletion of MRE11, part of the highly conserved RMX complex that aids in sensing and repairing double strand breaks in budding yeasts, enhanced sensitivity to gamma radiation (gamma-ray) (detection threshold of 50Gy) and camptothecin. The mre11Delta phenotype dominated in mag1Deltamre11Delta strains. Through the deletions, we were able to engineer increased selectivity to alkylating agents, gamma-ray, and camptothecin, since increased sensitivity to one type of damage did not alter the quantitative response to other genotoxins. The enhancements to the HUG1P-GFP system did not affect its ability to detect several other DNA damaging agents, including 1,2-dimethyl hydrazine (SDMH), phleomycin, and hydroxyurea (HU), or affect its lack of response to the potentially non-genotoxic carcinogen formaldehyde." @default.
- W2022946064 created "2016-06-24" @default.
- W2022946064 creator A5022974865 @default.
- W2022946064 creator A5024507536 @default.
- W2022946064 creator A5062284472 @default.
- W2022946064 date "2008-12-01" @default.
- W2022946064 modified "2023-10-09" @default.
- W2022946064 title "Deletion of MAG1 and MRE11 enhances the sensitivity of the Saccharomyces cerevisiae HUG1P-GFP promoter-reporter construct to genotoxicity" @default.
- W2022946064 cites W178587492 @default.
- W2022946064 cites W1832152442 @default.
- W2022946064 cites W1850362640 @default.
- W2022946064 cites W1934366607 @default.
- W2022946064 cites W1974933116 @default.
- W2022946064 cites W1979587131 @default.
- W2022946064 cites W1988810179 @default.
- W2022946064 cites W1990230487 @default.
- W2022946064 cites W1991586824 @default.
- W2022946064 cites W1991985168 @default.
- W2022946064 cites W2001190858 @default.
- W2022946064 cites W2003411878 @default.
- W2022946064 cites W2007749440 @default.
- W2022946064 cites W2010081095 @default.
- W2022946064 cites W2027282239 @default.
- W2022946064 cites W2038156068 @default.
- W2022946064 cites W2053313583 @default.
- W2022946064 cites W2067022597 @default.
- W2022946064 cites W2068933505 @default.
- W2022946064 cites W2074246747 @default.
- W2022946064 cites W2082087003 @default.
- W2022946064 cites W2097254193 @default.
- W2022946064 cites W2098980010 @default.
- W2022946064 cites W2110555186 @default.
- W2022946064 cites W2115958000 @default.
- W2022946064 cites W2129787793 @default.
- W2022946064 cites W2133701273 @default.
- W2022946064 cites W2142645687 @default.
- W2022946064 cites W2162735386 @default.
- W2022946064 cites W4240546531 @default.
- W2022946064 doi "https://doi.org/10.1016/j.bios.2008.06.033" @default.
- W2022946064 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4526160" @default.
- W2022946064 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18693109" @default.
- W2022946064 hasPublicationYear "2008" @default.
- W2022946064 type Work @default.
- W2022946064 sameAs 2022946064 @default.
- W2022946064 citedByCount "13" @default.
- W2022946064 countsByYear W20229460642012 @default.
- W2022946064 countsByYear W20229460642013 @default.
- W2022946064 countsByYear W20229460642014 @default.
- W2022946064 countsByYear W20229460642016 @default.
- W2022946064 countsByYear W20229460642018 @default.
- W2022946064 crossrefType "journal-article" @default.
- W2022946064 hasAuthorship W2022946064A5022974865 @default.
- W2022946064 hasAuthorship W2022946064A5024507536 @default.
- W2022946064 hasAuthorship W2022946064A5062284472 @default.
- W2022946064 hasBestOaLocation W20229460642 @default.
- W2022946064 hasConcept C104317684 @default.
- W2022946064 hasConcept C134935766 @default.
- W2022946064 hasConcept C143425029 @default.
- W2022946064 hasConcept C150194340 @default.
- W2022946064 hasConcept C153911025 @default.
- W2022946064 hasConcept C161733203 @default.
- W2022946064 hasConcept C185592680 @default.
- W2022946064 hasConcept C187206112 @default.
- W2022946064 hasConcept C2776900477 @default.
- W2022946064 hasConcept C2777576037 @default.
- W2022946064 hasConcept C2779682216 @default.
- W2022946064 hasConcept C552990157 @default.
- W2022946064 hasConcept C55493867 @default.
- W2022946064 hasConcept C86803240 @default.
- W2022946064 hasConceptScore W2022946064C104317684 @default.
- W2022946064 hasConceptScore W2022946064C134935766 @default.
- W2022946064 hasConceptScore W2022946064C143425029 @default.
- W2022946064 hasConceptScore W2022946064C150194340 @default.
- W2022946064 hasConceptScore W2022946064C153911025 @default.
- W2022946064 hasConceptScore W2022946064C161733203 @default.
- W2022946064 hasConceptScore W2022946064C185592680 @default.
- W2022946064 hasConceptScore W2022946064C187206112 @default.
- W2022946064 hasConceptScore W2022946064C2776900477 @default.
- W2022946064 hasConceptScore W2022946064C2777576037 @default.
- W2022946064 hasConceptScore W2022946064C2779682216 @default.
- W2022946064 hasConceptScore W2022946064C552990157 @default.
- W2022946064 hasConceptScore W2022946064C55493867 @default.
- W2022946064 hasConceptScore W2022946064C86803240 @default.
- W2022946064 hasIssue "4" @default.
- W2022946064 hasLocation W20229460641 @default.
- W2022946064 hasLocation W20229460642 @default.
- W2022946064 hasLocation W20229460643 @default.
- W2022946064 hasLocation W20229460644 @default.
- W2022946064 hasOpenAccess W2022946064 @default.
- W2022946064 hasPrimaryLocation W20229460641 @default.
- W2022946064 hasRelatedWork W2007786480 @default.
- W2022946064 hasRelatedWork W2014644281 @default.
- W2022946064 hasRelatedWork W2021717397 @default.
- W2022946064 hasRelatedWork W2077416184 @default.
- W2022946064 hasRelatedWork W2086386402 @default.
- W2022946064 hasRelatedWork W2512850011 @default.
- W2022946064 hasRelatedWork W3031599833 @default.
- W2022946064 hasRelatedWork W3141399017 @default.