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- W2022946887 abstract "Single-chain Fv (scFv) antibody'(Ab) fragments were transiently produced in COS-1 cells utilizing a mammalian expression vector featuring a murine immunoglobulin (Ig) light-chain leader sequence for efficient secretion and a murine Ig kappa constant domain (IgCκ) for detection. Several hundred milliliters of supernatants from large-scale COS cell transfections were sufficient to purify the scFv::IgCK fusion proteins by one-step affinity chromatography utilizing an immobilized rat anti-mouse IgCκ monoclonal Ab. Furthermore, the murine IgCκ domain allowed for accurate quantification of the scFv::IgCκ fusion protein secreted into the COS cell supernatant by a sandwich enzyme-linked immunosorbent assay (S-ELISA)." @default.
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- W2022946887 date "1995-12-01" @default.
- W2022946887 modified "2023-09-27" @default.
- W2022946887 title "A COS-cell-based system for rapid production and quantification of scFv::IgCκ antibody fragments" @default.
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- W2022946887 doi "https://doi.org/10.1016/0378-1119(95)00638-9" @default.
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