FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022949306> ?p ?o ?g. }

• W2022949306 endingPage "3386" @default.
• W2022949306 startingPage "3373" @default.
• W2022949306 abstract "2'-O-Methyloligoribonucleotides have been synthesised on solid phase from base protected 5'-O-dimethoxytrityl-2'-O-methylribonucleoside-3'-O-(2-cyanoethyl N,N-diisopropylphosphoramidites) using 5-(4-nitrophenyl)-1H-tetrazole as activator. Coupling yields greater than 99% were achieved, as judged by trityl cation release. The preparation of a modified 2'-deoxycytidine building block bearing an N4-(5-trifluoroacetylaminopentyl) spacer is also described. The latter compound enabled the chemical synthesis of 2'-O-methyloligoribonucleotide probes carrying several 5'- terminal biotinylation sites (in general four modified residues were used), which can be conveniently 32P end-labelled enzymatically using polynucleotide kinase. Used in conjunction with streptavidin-containing derivatives, such biotinylated probes have important applications in biochemical purification and electron microscopy of RNA-protein complexes. The 2'-O-methyloligoribonucleotides are completely resistant to degradation by either RNA or DNA specific nucleases. In contrast, nucleases with dual RNA/DNA specificity show a complete spectrum of cleavage rates." @default.
• W2022949306 created "2016-06-24" @default.
• W2022949306 creator A5014112981 @default.
• W2022949306 creator A5018657526 @default.
• W2022949306 creator A5042871682 @default.
• W2022949306 creator A5083010445 @default.
• W2022949306 creator A5084924302 @default.
• W2022949306 date "1989-01-01" @default.
• W2022949306 modified "2023-10-05" @default.
• W2022949306 title "Highly efficient chemical synthesis of 2′-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases" @default.
• W2022949306 cites W1551358827 @default.
• W2022949306 cites W1984286262 @default.
• W2022949306 cites W1986115620 @default.
• W2022949306 cites W1986686615 @default.
• W2022949306 cites W2010299178 @default.
• W2022949306 cites W2023971542 @default.
• W2022949306 cites W2040455172 @default.
• W2022949306 cites W2044694730 @default.
• W2022949306 cites W2045822162 @default.
• W2022949306 cites W2084285144 @default.
• W2022949306 cites W2320845554 @default.
• W2022949306 cites W2406599128 @default.
• W2022949306 cites W2413804611 @default.
• W2022949306 cites W2434682130 @default.
• W2022949306 doi "https://doi.org/10.1093/nar/17.9.3373" @default.
• W2022949306 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/317781" @default.
• W2022949306 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2726482" @default.
• W2022949306 hasPublicationYear "1989" @default.
• W2022949306 type Work @default.
• W2022949306 sameAs 2022949306 @default.
• W2022949306 citedByCount "257" @default.
• W2022949306 countsByYear W20229493062012 @default.
• W2022949306 countsByYear W20229493062013 @default.
• W2022949306 countsByYear W20229493062014 @default.
• W2022949306 countsByYear W20229493062015 @default.
• W2022949306 countsByYear W20229493062016 @default.
• W2022949306 countsByYear W20229493062017 @default.
• W2022949306 countsByYear W20229493062018 @default.
• W2022949306 countsByYear W20229493062019 @default.
• W2022949306 countsByYear W20229493062020 @default.
• W2022949306 countsByYear W20229493062021 @default.
• W2022949306 countsByYear W20229493062022 @default.
• W2022949306 countsByYear W20229493062023 @default.
• W2022949306 crossrefType "journal-article" @default.
• W2022949306 hasAuthorship W2022949306A5014112981 @default.
• W2022949306 hasAuthorship W2022949306A5018657526 @default.
• W2022949306 hasAuthorship W2022949306A5042871682 @default.
• W2022949306 hasAuthorship W2022949306A5083010445 @default.
• W2022949306 hasAuthorship W2022949306A5084924302 @default.
• W2022949306 hasBestOaLocation W20229493062 @default.
• W2022949306 hasConcept C104317684 @default.
• W2022949306 hasConcept C111921641 @default.
• W2022949306 hasConcept C129312508 @default.
• W2022949306 hasConcept C151730666 @default.
• W2022949306 hasConcept C153911025 @default.
• W2022949306 hasConcept C175156509 @default.
• W2022949306 hasConcept C185592680 @default.
• W2022949306 hasConcept C204628709 @default.
• W2022949306 hasConcept C21951064 @default.
• W2022949306 hasConcept C24107716 @default.
• W2022949306 hasConcept C3017666073 @default.
• W2022949306 hasConcept C43369102 @default.
• W2022949306 hasConcept C54871578 @default.
• W2022949306 hasConcept C552990157 @default.
• W2022949306 hasConcept C55493867 @default.
• W2022949306 hasConcept C67705224 @default.
• W2022949306 hasConcept C86803240 @default.
• W2022949306 hasConceptScore W2022949306C104317684 @default.
• W2022949306 hasConceptScore W2022949306C111921641 @default.
• W2022949306 hasConceptScore W2022949306C129312508 @default.
• W2022949306 hasConceptScore W2022949306C151730666 @default.
• W2022949306 hasConceptScore W2022949306C153911025 @default.
• W2022949306 hasConceptScore W2022949306C175156509 @default.
• W2022949306 hasConceptScore W2022949306C185592680 @default.
• W2022949306 hasConceptScore W2022949306C204628709 @default.
• W2022949306 hasConceptScore W2022949306C21951064 @default.
• W2022949306 hasConceptScore W2022949306C24107716 @default.
• W2022949306 hasConceptScore W2022949306C3017666073 @default.
• W2022949306 hasConceptScore W2022949306C43369102 @default.
• W2022949306 hasConceptScore W2022949306C54871578 @default.
• W2022949306 hasConceptScore W2022949306C552990157 @default.
• W2022949306 hasConceptScore W2022949306C55493867 @default.
• W2022949306 hasConceptScore W2022949306C67705224 @default.
• W2022949306 hasConceptScore W2022949306C86803240 @default.
• W2022949306 hasIssue "9" @default.
• W2022949306 hasLocation W20229493061 @default.
• W2022949306 hasLocation W20229493062 @default.
• W2022949306 hasLocation W20229493063 @default.
• W2022949306 hasLocation W20229493064 @default.
• W2022949306 hasOpenAccess W2022949306 @default.
• W2022949306 hasPrimaryLocation W20229493061 @default.
• W2022949306 hasRelatedWork W1988220558 @default.
• W2022949306 hasRelatedWork W2002473487 @default.
• W2022949306 hasRelatedWork W2008383097 @default.
• W2022949306 hasRelatedWork W2059177774 @default.
• W2022949306 hasRelatedWork W2062500327 @default.
• W2022949306 hasRelatedWork W2083193178 @default.
• W2022949306 hasRelatedWork W2086743682 @default.

• next