Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022959290> ?p ?o ?g. }
- W2022959290 endingPage "3250" @default.
- W2022959290 startingPage "3242" @default.
- W2022959290 abstract "Nef, an accessory protein from human immunodeficiency virus type 1, is critical for optimal viral replication and pathogenesis. Here, we analyzed the influence of full-length myristoylated and nonmyristoylated Nef on artificial lipid bilayers composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). By means of cosedimentation assays, we found that neither nonmyristoylated nor myristoylated Nef stably binds to POPC unilamellar vesicles. Time-resolved ellipsometry rather indicates that the proteins perturb the assembly of POPC planar bilayers. This observation was corroborated by fluorescence and scanning force microscopy, suggesting that membrane disordering occurs upon interaction of full-length myristoylated and nonmyristoylated Nef with planar POPC membranes immobilized on SiO2 surfaces resulting in loss of material from the surface. The membrane perturbations were further investigated by vesicle release experiments, demonstrating that the disordering results in defects through which the fluorophor carboxyfluorescein can pass. From these results, we conclude that Nef is capable of disordering and perturbing lipid membranes and that the myristoyl group is not the decisive determinant for the action of the protein on lipid membranes." @default.
- W2022959290 created "2016-06-24" @default.
- W2022959290 creator A5011493742 @default.
- W2022959290 creator A5041651362 @default.
- W2022959290 creator A5059323669 @default.
- W2022959290 creator A5068603027 @default.
- W2022959290 creator A5071355052 @default.
- W2022959290 creator A5074615704 @default.
- W2022959290 creator A5084418237 @default.
- W2022959290 date "2009-04-01" @default.
- W2022959290 modified "2023-10-10" @default.
- W2022959290 title "HIV-1 Nef Perturbs Artificial Membranes: Investigation of the Contribution of the Myristoyl Anchor" @default.
- W2022959290 cites W167846769 @default.
- W2022959290 cites W1966680129 @default.
- W2022959290 cites W1969436018 @default.
- W2022959290 cites W1976247350 @default.
- W2022959290 cites W1979542915 @default.
- W2022959290 cites W1981298192 @default.
- W2022959290 cites W1986516374 @default.
- W2022959290 cites W1997094324 @default.
- W2022959290 cites W2001711063 @default.
- W2022959290 cites W2008264122 @default.
- W2022959290 cites W2017669430 @default.
- W2022959290 cites W2028748655 @default.
- W2022959290 cites W2034010375 @default.
- W2022959290 cites W2034637556 @default.
- W2022959290 cites W2035096705 @default.
- W2022959290 cites W2038015704 @default.
- W2022959290 cites W2038126024 @default.
- W2022959290 cites W2039300275 @default.
- W2022959290 cites W2040224182 @default.
- W2022959290 cites W2041078155 @default.
- W2022959290 cites W2048779945 @default.
- W2022959290 cites W2050428252 @default.
- W2022959290 cites W2050737543 @default.
- W2022959290 cites W2054395379 @default.
- W2022959290 cites W2060629825 @default.
- W2022959290 cites W2061502091 @default.
- W2022959290 cites W2064557078 @default.
- W2022959290 cites W2065658041 @default.
- W2022959290 cites W2068807057 @default.
- W2022959290 cites W2070335320 @default.
- W2022959290 cites W2074852719 @default.
- W2022959290 cites W2077484316 @default.
- W2022959290 cites W2077704737 @default.
- W2022959290 cites W2086264258 @default.
- W2022959290 cites W2087070066 @default.
- W2022959290 cites W2090429239 @default.
- W2022959290 cites W2095111901 @default.
- W2022959290 cites W2096344281 @default.
- W2022959290 cites W2112612176 @default.
- W2022959290 cites W2113076274 @default.
- W2022959290 cites W2118276643 @default.
- W2022959290 cites W2119021394 @default.
- W2022959290 cites W2124737249 @default.
- W2022959290 cites W2131413819 @default.
- W2022959290 cites W2143430925 @default.
- W2022959290 cites W2152210196 @default.
- W2022959290 cites W2335501329 @default.
- W2022959290 cites W2342220526 @default.
- W2022959290 doi "https://doi.org/10.1016/j.bpj.2008.12.3947" @default.
- W2022959290 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2718304" @default.
- W2022959290 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19383468" @default.
- W2022959290 hasPublicationYear "2009" @default.
- W2022959290 type Work @default.
- W2022959290 sameAs 2022959290 @default.
- W2022959290 citedByCount "5" @default.
- W2022959290 countsByYear W20229592902015 @default.
- W2022959290 crossrefType "journal-article" @default.
- W2022959290 hasAuthorship W2022959290A5011493742 @default.
- W2022959290 hasAuthorship W2022959290A5041651362 @default.
- W2022959290 hasAuthorship W2022959290A5059323669 @default.
- W2022959290 hasAuthorship W2022959290A5068603027 @default.
- W2022959290 hasAuthorship W2022959290A5071355052 @default.
- W2022959290 hasAuthorship W2022959290A5074615704 @default.
- W2022959290 hasAuthorship W2022959290A5084418237 @default.
- W2022959290 hasBestOaLocation W20229592901 @default.
- W2022959290 hasConcept C12554922 @default.
- W2022959290 hasConcept C130316041 @default.
- W2022959290 hasConcept C185154212 @default.
- W2022959290 hasConcept C185592680 @default.
- W2022959290 hasConcept C2780943371 @default.
- W2022959290 hasConcept C39944091 @default.
- W2022959290 hasConcept C41625074 @default.
- W2022959290 hasConcept C55493867 @default.
- W2022959290 hasConcept C77207865 @default.
- W2022959290 hasConcept C8010536 @default.
- W2022959290 hasConcept C86803240 @default.
- W2022959290 hasConceptScore W2022959290C12554922 @default.
- W2022959290 hasConceptScore W2022959290C130316041 @default.
- W2022959290 hasConceptScore W2022959290C185154212 @default.
- W2022959290 hasConceptScore W2022959290C185592680 @default.
- W2022959290 hasConceptScore W2022959290C2780943371 @default.
- W2022959290 hasConceptScore W2022959290C39944091 @default.
- W2022959290 hasConceptScore W2022959290C41625074 @default.
- W2022959290 hasConceptScore W2022959290C55493867 @default.
- W2022959290 hasConceptScore W2022959290C77207865 @default.
- W2022959290 hasConceptScore W2022959290C8010536 @default.