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- W2022960176 endingPage "8525" @default.
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- W2022960176 abstract "A method for low-level, low-potential electrochemical detection of hydrogen peroxide using a chemically activated redox mediator is presented. This method is unique in that it utilizes a mediator, Amplex Red, which is only redox-active when chemically oxidized by H2O2 in the presence of the enzyme horseradish peroxidase (HRP). When employed in concert with microelectrode square wave voltammetry to optimize sensing at ultralow concentrations (<1 microM), this method exhibits marked improvements in analytical sensitivity and detection limits (limit of detection as low as 8 pM) over existing protocols. Sensing schemes incorporating both freely diffusing and immobilized HRP are evaluated, and the resulting analytical sensitivities are 1.22 +/- 0.04 and (2.1 +/- 0.6) x 10(-1) microA/(microM mm2), respectively, for peroxide concentrations in the high picomolar to low micromolar range. A second linear region exists for lower peroxide concentrations. Furthermore, quantitative enzyme kinetics analysis using Michaelis-Menten parameters is possible through interpretation of data collected in this scheme. Km values for soluble and immobilized HRP were 84 +/- 13 and 504 +/- 19 microM, respectively. This method is amenable to any biological detection scheme that generates hydrogen peroxide as a reactive product." @default.
- W2022960176 created "2016-06-24" @default.
- W2022960176 creator A5064864080 @default.
- W2022960176 creator A5088934376 @default.
- W2022960176 date "2006-11-10" @default.
- W2022960176 modified "2023-10-03" @default.
- W2022960176 title "Picomolar Peroxide Detection Using a Chemically Activated Redox Mediator and Square Wave Voltammetry" @default.
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- W2022960176 doi "https://doi.org/10.1021/ac061483d" @default.
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