FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022969655> ?p ?o ?g. }

• W2022969655 endingPage "175" @default.
• W2022969655 startingPage "168" @default.
• W2022969655 abstract "<i>Purpose:</i> The aim of this study was to evaluate the suppression effect of survivin shRNA on the expression of the survivin gene in the human laryngeal cancer cell line Hep-2. <i>Procedures:</i> 60 cases of laryngeal squamous-cell carcinoma (LSCC) and 10 cases of normal laryngeal mucosa were examined using immunohistochemistry to determine whether the expression of survivin correlated with tumorigenesis. Three plasmid vectors of short hairpin RNA (shRNA) specific for survivin were designed and generated. Western blot and real-time PCR analysis of survivin expression in Hep-2 cells was performed 48 h after transfection. The growth curve was used to determine the cell proliferation. Propidium iodide (PI) single staining was applied to detect the cell cycle. The apoptosis of the cells was analyzed by flow cytometry with the FITC-annexin-V/PI double staining and PI single staining. <i>Results:</i> 68.33% (41 out of 60) of tumors were positive for survivin expression and significantly associated with lymph node metastasis and advanced stage. In contrast, no expression of survivin in normal mucosa was detected. Transfection of Hep-2 cells with survivin shRNA significantly inhibited survivin expression at both the mRNA and the protein level in Hep-2 cells. Downregulation of survivin resulted in increasing the apoptosis index, but the results showed no obvious influence on cell cycle. <i>Conclusions:</i> This study demonstrates that survivin shRNA effectively inhibits survivin gene expression in Hep-2 cells leading to growth suppression and apoptotic induction in Hep-2 cells." @default.
• W2022969655 created "2016-06-24" @default.
• W2022969655 creator A5003952522 @default.
• W2022969655 creator A5007239909 @default.
• W2022969655 creator A5037269791 @default.
• W2022969655 creator A5040593698 @default.
• W2022969655 creator A5063298963 @default.
• W2022969655 creator A5071407050 @default.
• W2022969655 creator A5085352453 @default.
• W2022969655 date "2008-01-01" @default.
• W2022969655 modified "2023-10-10" @default.
• W2022969655 title "Suppression of Survivin Expression by Short Hairpin RNA Induces Apoptosis in Human Laryngeal Carcinoma Cells" @default.
• W2022969655 cites W1967065056 @default.
• W2022969655 cites W1970852630 @default.
• W2022969655 cites W1984545745 @default.
• W2022969655 cites W1985111376 @default.
• W2022969655 cites W1992045438 @default.
• W2022969655 cites W1994017228 @default.
• W2022969655 cites W2002503549 @default.
• W2022969655 cites W2017991840 @default.
• W2022969655 cites W2035523654 @default.
• W2022969655 cites W2042409437 @default.
• W2022969655 cites W2048525179 @default.
• W2022969655 cites W2051624438 @default.
• W2022969655 cites W2076132968 @default.
• W2022969655 cites W2167490100 @default.
• W2022969655 cites W2171109371 @default.
• W2022969655 doi "https://doi.org/10.1159/000124290" @default.
• W2022969655 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18391576" @default.
• W2022969655 hasPublicationYear "2008" @default.
• W2022969655 type Work @default.
• W2022969655 sameAs 2022969655 @default.
• W2022969655 citedByCount "11" @default.
• W2022969655 countsByYear W20229696552012 @default.
• W2022969655 countsByYear W20229696552013 @default.
• W2022969655 countsByYear W20229696552015 @default.
• W2022969655 countsByYear W20229696552018 @default.
• W2022969655 countsByYear W20229696552022 @default.
• W2022969655 crossrefType "journal-article" @default.
• W2022969655 hasAuthorship W2022969655A5003952522 @default.
• W2022969655 hasAuthorship W2022969655A5007239909 @default.
• W2022969655 hasAuthorship W2022969655A5037269791 @default.
• W2022969655 hasAuthorship W2022969655A5040593698 @default.
• W2022969655 hasAuthorship W2022969655A5063298963 @default.
• W2022969655 hasAuthorship W2022969655A5071407050 @default.
• W2022969655 hasAuthorship W2022969655A5085352453 @default.
• W2022969655 hasConcept C121608353 @default.
• W2022969655 hasConcept C153911025 @default.
• W2022969655 hasConcept C173396325 @default.
• W2022969655 hasConcept C185592680 @default.
• W2022969655 hasConcept C190232843 @default.
• W2022969655 hasConcept C190283241 @default.
• W2022969655 hasConcept C2775934118 @default.
• W2022969655 hasConcept C2775975398 @default.
• W2022969655 hasConcept C29537977 @default.
• W2022969655 hasConcept C31573885 @default.
• W2022969655 hasConcept C502942594 @default.
• W2022969655 hasConcept C54009773 @default.
• W2022969655 hasConcept C54355233 @default.
• W2022969655 hasConcept C55493867 @default.
• W2022969655 hasConcept C555283112 @default.
• W2022969655 hasConcept C62112901 @default.
• W2022969655 hasConcept C81885089 @default.
• W2022969655 hasConcept C86803240 @default.
• W2022969655 hasConceptScore W2022969655C121608353 @default.
• W2022969655 hasConceptScore W2022969655C153911025 @default.
• W2022969655 hasConceptScore W2022969655C173396325 @default.
• W2022969655 hasConceptScore W2022969655C185592680 @default.
• W2022969655 hasConceptScore W2022969655C190232843 @default.
• W2022969655 hasConceptScore W2022969655C190283241 @default.
• W2022969655 hasConceptScore W2022969655C2775934118 @default.
• W2022969655 hasConceptScore W2022969655C2775975398 @default.
• W2022969655 hasConceptScore W2022969655C29537977 @default.
• W2022969655 hasConceptScore W2022969655C31573885 @default.
• W2022969655 hasConceptScore W2022969655C502942594 @default.
• W2022969655 hasConceptScore W2022969655C54009773 @default.
• W2022969655 hasConceptScore W2022969655C54355233 @default.
• W2022969655 hasConceptScore W2022969655C55493867 @default.
• W2022969655 hasConceptScore W2022969655C555283112 @default.
• W2022969655 hasConceptScore W2022969655C62112901 @default.
• W2022969655 hasConceptScore W2022969655C81885089 @default.
• W2022969655 hasConceptScore W2022969655C86803240 @default.
• W2022969655 hasIssue "3" @default.
• W2022969655 hasLocation W20229696551 @default.
• W2022969655 hasLocation W20229696552 @default.
• W2022969655 hasOpenAccess W2022969655 @default.
• W2022969655 hasPrimaryLocation W20229696551 @default.
• W2022969655 hasRelatedWork W2377933702 @default.
• W2022969655 hasRelatedWork W2388731891 @default.
• W2022969655 hasRelatedWork W2529824788 @default.
• W2022969655 hasRelatedWork W2580481553 @default.
• W2022969655 hasRelatedWork W3028888098 @default.
• W2022969655 hasRelatedWork W3032354441 @default.
• W2022969655 hasRelatedWork W3143574342 @default.
• W2022969655 hasRelatedWork W3151141465 @default.
• W2022969655 hasRelatedWork W3171350128 @default.
• W2022969655 hasRelatedWork W4254004086 @default.
• W2022969655 hasVolume "70" @default.

• next