Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022981320> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2022981320 endingPage "396" @default.
- W2022981320 startingPage "373" @default.
- W2022981320 abstract "Abstract A procedure has been developed by which the hybrid formed between a labeled RNA and complementary DNA can be selectively separated from all other single and double-stranded nucleic acids. We describe the application of this procedure to the quantitative determination of labeled avian tumor virus RNA. Purified DNA complementary to avian myeloblastosis virus RNA is extended at its 3′ terminus with 40 to 60 dCMP residues, using terminal deoxynucleotidyl-transferase. The elongated DNA is annealed with the labeled nucleic acid preparation and the mixture is passed through a column of Sephadex to which poly(I) has been covalently bound. The poly(I) retains the specific RNA-DNA hybrids by virtue of their poly(C) extension. The column is washed with RNAase to degrade nonhybridized RNA, the RNA retained on the column is eluted with formamide and its radioactivity is determined. The background hybridization was reduced to 0.003 to 0.008% by addition of oligo(C) 5.20 to the hybridization mixture and by carrying out the adsorption to the poly(I)-Sephadex column in the presence of poly(U). The hybridization efficiency was about 50%. The content of radioactive Rous sarcoma virus-specific RNA was determined in infected and uninfected cells after labeling with [ 3 H]uridine for two hours. The content of labeled virus-specific RNA in infected cells was 0.6 to 0.9% and 0.05% in uninfected cells. The value found for monkey cell RNA was 0.009%. This method can be used for the detection of hybrids between labeled RNA and complementary DNAs too short to allow quantitation by conventional methods. If the RNAase step is omitted the procedure can be used for the isolation of any RNA for which a complementary DNA is available, as well as for its precursor." @default.
- W2022981320 created "2016-06-24" @default.
- W2022981320 creator A5047810199 @default.
- W2022981320 creator A5052603452 @default.
- W2022981320 creator A5065227576 @default.
- W2022981320 creator A5068938871 @default.
- W2022981320 creator A5084617141 @default.
- W2022981320 date "1974-06-01" @default.
- W2022981320 modified "2023-10-14" @default.
- W2022981320 title "A new approach to the isolation of RNA-DNA hybrids and its application to the quantitative determination of labeled tumor virus RNA" @default.
- W2022981320 cites W1568309631 @default.
- W2022981320 cites W1692048711 @default.
- W2022981320 cites W1795036143 @default.
- W2022981320 cites W1883779995 @default.
- W2022981320 cites W1894559991 @default.
- W2022981320 cites W1985959206 @default.
- W2022981320 cites W2005389848 @default.
- W2022981320 cites W2010416376 @default.
- W2022981320 cites W2020953080 @default.
- W2022981320 cites W2032556026 @default.
- W2022981320 cites W2039492390 @default.
- W2022981320 cites W2051611713 @default.
- W2022981320 cites W2054448437 @default.
- W2022981320 cites W2055801203 @default.
- W2022981320 cites W2074135297 @default.
- W2022981320 cites W2087816060 @default.
- W2022981320 cites W2088974713 @default.
- W2022981320 cites W2093232268 @default.
- W2022981320 cites W2108594321 @default.
- W2022981320 cites W2141698253 @default.
- W2022981320 doi "https://doi.org/10.1016/0022-2836(74)90026-6" @default.
- W2022981320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4369723" @default.
- W2022981320 hasPublicationYear "1974" @default.
- W2022981320 type Work @default.
- W2022981320 sameAs 2022981320 @default.
- W2022981320 citedByCount "36" @default.
- W2022981320 crossrefType "journal-article" @default.
- W2022981320 hasAuthorship W2022981320A5047810199 @default.
- W2022981320 hasAuthorship W2022981320A5052603452 @default.
- W2022981320 hasAuthorship W2022981320A5065227576 @default.
- W2022981320 hasAuthorship W2022981320A5068938871 @default.
- W2022981320 hasAuthorship W2022981320A5084617141 @default.
- W2022981320 hasConcept C104317684 @default.
- W2022981320 hasConcept C153911025 @default.
- W2022981320 hasConcept C159047783 @default.
- W2022981320 hasConcept C2522874641 @default.
- W2022981320 hasConcept C2775941552 @default.
- W2022981320 hasConcept C54355233 @default.
- W2022981320 hasConcept C552990157 @default.
- W2022981320 hasConcept C67705224 @default.
- W2022981320 hasConcept C70721500 @default.
- W2022981320 hasConcept C86803240 @default.
- W2022981320 hasConcept C89423630 @default.
- W2022981320 hasConceptScore W2022981320C104317684 @default.
- W2022981320 hasConceptScore W2022981320C153911025 @default.
- W2022981320 hasConceptScore W2022981320C159047783 @default.
- W2022981320 hasConceptScore W2022981320C2522874641 @default.
- W2022981320 hasConceptScore W2022981320C2775941552 @default.
- W2022981320 hasConceptScore W2022981320C54355233 @default.
- W2022981320 hasConceptScore W2022981320C552990157 @default.
- W2022981320 hasConceptScore W2022981320C67705224 @default.
- W2022981320 hasConceptScore W2022981320C70721500 @default.
- W2022981320 hasConceptScore W2022981320C86803240 @default.
- W2022981320 hasConceptScore W2022981320C89423630 @default.
- W2022981320 hasIssue "2" @default.
- W2022981320 hasLocation W20229813201 @default.
- W2022981320 hasLocation W20229813202 @default.
- W2022981320 hasOpenAccess W2022981320 @default.
- W2022981320 hasPrimaryLocation W20229813201 @default.
- W2022981320 hasRelatedWork W1976736675 @default.
- W2022981320 hasRelatedWork W1991523530 @default.
- W2022981320 hasRelatedWork W2002128513 @default.
- W2022981320 hasRelatedWork W2002473487 @default.
- W2022981320 hasRelatedWork W2016551366 @default.
- W2022981320 hasRelatedWork W2020824267 @default.
- W2022981320 hasRelatedWork W2057739827 @default.
- W2022981320 hasRelatedWork W2070232054 @default.
- W2022981320 hasRelatedWork W2142935324 @default.
- W2022981320 hasRelatedWork W2092874662 @default.
- W2022981320 hasVolume "86" @default.
- W2022981320 isParatext "false" @default.
- W2022981320 isRetracted "false" @default.
- W2022981320 magId "2022981320" @default.
- W2022981320 workType "article" @default.