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- W2022982578 abstract "The catalytic activity of serum L-lactate dehydrogenase (LDH), was determined by monitoring the NADH produced by LDH with bacterial bioluminescent enzymes immobilized on a nylon coil. The LDH reaction of L-lactate with NAD took place in a flow-through mixing coil that preceded the bioluminescent detector coil. The response was linear from 1 to 5000 U/l at 37 degrees C and from 3 to 2000 U/l at 25 degrees C. The intra- and inter-assay reproducibility (CV%) were less than 10% and recovery range was 92% to 110%. The results agreed well with those obtained with a spectrophotometric method." @default.
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- W2022982578 date "1989-04-01" @default.
- W2022982578 modified "2023-09-27" @default.
- W2022982578 title "Bioluminescent flow sensors:L-lactate dehydrogenase activity determination in serum" @default.
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- W2022982578 doi "https://doi.org/10.1002/bio.1170030202" @default.
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