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- W2022992227 abstract "We investigated the effect of lead nitrate (0.1, 1.0, 10 or 20 μM) on the metabolism of glycosaminoglycans (GAG) in confluent cultures of bovine aortic endothelial cells. It was found that lead at 10 and 20 μM significantly decreased the accumulation of [35S]sulfate-labeled GAG ([35S]GAG) both in teh cell layer and the medium after a 24-h culture. A time course study showed that 10 μM lead decreased the accumulation of [3H]glucosamine-labeled GAG both in the cell layer and the medium after 24 h and longer. The release of [35S]GAG from the cell layer during the last 3 h of a 24 h culture was not changed by lead. The detachment of [3H]thymidine-labeled cells from the monolayer was unaffected by lead. It was shown that lead at 10 μM decreased both herapan sulfate and the other GAG in the cell layer; the former was more sensitive to lead treatment. Lead at 20 μM and below failed to increase the release of lactate dehydrogenase, suggesting that non-specific cell damage was not caused by lead. From these results, it was suggests that lead decreases endothelial cell harapan sulafte content through a decrease in the GAG production without a non-specific cell damage. Lead may be a risk factor of vascular disorders." @default.
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- W2022992227 date "1991-01-01" @default.
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- W2022992227 title "Effect of lead on the glycosaminoglycans metabolism of bovine aortic endothelial cells in culture" @default.
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- W2022992227 doi "https://doi.org/10.1016/0300-483x(91)90073-a" @default.
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