FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022995241> ?p ?o ?g. }

• W2022995241 endingPage "27" @default.
• W2022995241 startingPage "16" @default.
• W2022995241 abstract "We have established an easy real-time PCR assay, which allows the precise quantification of changes in the expression level of 6 relevant porcine cytokines, and 3 housekeeping genes. This assay simultaneously detects 9 sequences by measuring 3 x 3 targets in a triplex-format. The mRNA of the lymphokines IL-2, IL-4, IL-10, and IFN-gamma, of the proinflammatory cytokines IL-1alpha and IL-6, and of the housekeeping genes are quantified using TaqMan-probes by means of standard dilution series on the iCycler iQ. The standard consists of equal aliquots of the experimental cDNAs under investigation. Simultaneously the most suitable combination of 3 out of the four housekeeping genes beta-actin, HPRT, GAPDH, and cyclophilin can be selected, and their averaged expression values constitute a normalisation factor. The raw data of all targets of interest is then calculated relative to this normalisation factor, making eventual changes of the relative expression level of the single housekeeping genes controllable and quantifiable. We have applied this assay to quantify changes in the cytokine mRNA levels of porcine stimulated with various concentrations of LPS and ConA, known to induce different cytokine expression patterns. We have shown, that even small differences in the expression level (less than 2-fold) can be precisely quantified, and reveal statistically significant changes, when using the normalisation factor. This assay will be useful for studying changes in the expression of relevant porcine cytokines and will help to further improve the investigation of immune responses in the pig." @default.
• W2022995241 created "2016-06-24" @default.
• W2022995241 creator A5031631543 @default.
• W2022995241 creator A5049448761 @default.
• W2022995241 creator A5060776268 @default.
• W2022995241 creator A5061410834 @default.
• W2022995241 date "2005-11-01" @default.
• W2022995241 modified "2023-09-29" @default.
• W2022995241 title "Quantitative simultaneous multiplex real-time PCR for the detection of porcine cytokines" @default.
• W2022995241 cites W1577577364 @default.
• W2022995241 cites W1967255149 @default.
• W2022995241 cites W1972170391 @default.
• W2022995241 cites W1973308380 @default.
• W2022995241 cites W1991926326 @default.
• W2022995241 cites W2007778401 @default.
• W2022995241 cites W2018868619 @default.
• W2022995241 cites W2023384421 @default.
• W2022995241 cites W2025492442 @default.
• W2022995241 cites W2029128615 @default.
• W2022995241 cites W2032124268 @default.
• W2022995241 cites W2051725610 @default.
• W2022995241 cites W2054465323 @default.
• W2022995241 cites W2057550402 @default.
• W2022995241 cites W2068420331 @default.
• W2022995241 cites W2074899532 @default.
• W2022995241 cites W2088217295 @default.
• W2022995241 cites W2096298665 @default.
• W2022995241 cites W2112982595 @default.
• W2022995241 cites W2123247959 @default.
• W2022995241 cites W2128088040 @default.
• W2022995241 cites W2136054301 @default.
• W2022995241 cites W2143146123 @default.
• W2022995241 cites W2144040053 @default.
• W2022995241 cites W2145879504 @default.
• W2022995241 cites W2147636608 @default.
• W2022995241 cites W2156253798 @default.
• W2022995241 doi "https://doi.org/10.1016/j.jim.2005.06.021" @default.
• W2022995241 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16223507" @default.
• W2022995241 hasPublicationYear "2005" @default.
• W2022995241 type Work @default.
• W2022995241 sameAs 2022995241 @default.
• W2022995241 citedByCount "132" @default.
• W2022995241 countsByYear W20229952412012 @default.
• W2022995241 countsByYear W20229952412013 @default.
• W2022995241 countsByYear W20229952412014 @default.
• W2022995241 countsByYear W20229952412015 @default.
• W2022995241 countsByYear W20229952412016 @default.
• W2022995241 countsByYear W20229952412017 @default.
• W2022995241 countsByYear W20229952412018 @default.
• W2022995241 countsByYear W20229952412019 @default.
• W2022995241 countsByYear W20229952412020 @default.
• W2022995241 countsByYear W20229952412021 @default.
• W2022995241 countsByYear W20229952412022 @default.
• W2022995241 countsByYear W20229952412023 @default.
• W2022995241 crossrefType "journal-article" @default.
• W2022995241 hasAuthorship W2022995241A5031631543 @default.
• W2022995241 hasAuthorship W2022995241A5049448761 @default.
• W2022995241 hasAuthorship W2022995241A5060776268 @default.
• W2022995241 hasAuthorship W2022995241A5061410834 @default.
• W2022995241 hasConcept C104317684 @default.
• W2022995241 hasConcept C142724271 @default.
• W2022995241 hasConcept C143660080 @default.
• W2022995241 hasConcept C150194340 @default.
• W2022995241 hasConcept C153911025 @default.
• W2022995241 hasConcept C164027704 @default.
• W2022995241 hasConcept C184112429 @default.
• W2022995241 hasConcept C203014093 @default.
• W2022995241 hasConcept C204787440 @default.
• W2022995241 hasConcept C2776914184 @default.
• W2022995241 hasConcept C2778690821 @default.
• W2022995241 hasConcept C2779148672 @default.
• W2022995241 hasConcept C2781188995 @default.
• W2022995241 hasConcept C33922130 @default.
• W2022995241 hasConcept C48023723 @default.
• W2022995241 hasConcept C54355233 @default.
• W2022995241 hasConcept C70721500 @default.
• W2022995241 hasConcept C71924100 @default.
• W2022995241 hasConcept C86803240 @default.
• W2022995241 hasConceptScore W2022995241C104317684 @default.
• W2022995241 hasConceptScore W2022995241C142724271 @default.
• W2022995241 hasConceptScore W2022995241C143660080 @default.
• W2022995241 hasConceptScore W2022995241C150194340 @default.
• W2022995241 hasConceptScore W2022995241C153911025 @default.
• W2022995241 hasConceptScore W2022995241C164027704 @default.
• W2022995241 hasConceptScore W2022995241C184112429 @default.
• W2022995241 hasConceptScore W2022995241C203014093 @default.
• W2022995241 hasConceptScore W2022995241C204787440 @default.
• W2022995241 hasConceptScore W2022995241C2776914184 @default.
• W2022995241 hasConceptScore W2022995241C2778690821 @default.
• W2022995241 hasConceptScore W2022995241C2779148672 @default.
• W2022995241 hasConceptScore W2022995241C2781188995 @default.
• W2022995241 hasConceptScore W2022995241C33922130 @default.
• W2022995241 hasConceptScore W2022995241C48023723 @default.
• W2022995241 hasConceptScore W2022995241C54355233 @default.
• W2022995241 hasConceptScore W2022995241C70721500 @default.
• W2022995241 hasConceptScore W2022995241C71924100 @default.
• W2022995241 hasConceptScore W2022995241C86803240 @default.
• W2022995241 hasIssue "1-2" @default.

• next