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- W2023004623 abstract "GPR103 plays an important role in various tissues, while little information is available about the alternative splicing (AS) of its mRNA. In the present study, we used genomic PCR to identify the partial genomic locus of goose (Anser cygnoides) GPR103 and rapid amplification of cDNA ends (RACE)-PCR to identify five GPR103 variants, including the full-length variant (aGPR103-n) and four alternatively spliced variants (aGPR103-va, -vb, -vc and -vd). Sequence analysis showed that aGPR103-va and -vd are less likely to undergo nonsense-mediated mRNA decay, suggesting that they may be translated into truncated proteins. Quantitative real-time PCR (qRT-PCR) analysis revealed that the five variants are widely distributed in the brain and peripheral tissues of geese and show specific expression patterns. Thus, we here provide the first account of the GPR103 genomic locus and illustrate its transcriptional diversity and widespread distribution in geese." @default.
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- W2023004623 date "2014-05-01" @default.
- W2023004623 modified "2023-09-23" @default.
- W2023004623 title "Five novel variants of GPR103 and their expression in different tissues of goose ( Anser cygnoides )" @default.
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- W2023004623 doi "https://doi.org/10.1016/j.cbpb.2014.03.002" @default.
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