FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023006665> ?p ?o ?g. }

• W2023006665 endingPage "128" @default.
• W2023006665 startingPage "115" @default.
• W2023006665 abstract "To identify estrogen (E)-responsive genes that may play important roles in the sexual differentiation and maturation of the neuroendocrine hypothalamus, we used mRNA differential display PCR to analyze hypothalamic RNA derived from estrogen-sterilized rats (ESRs). Neonatal rats were s.c.-injected with 100 μg of 17β-estradiol-benzoate (EB) for 5 days. Approximately 300 out of more than 2000 RNAs examined displayed a differential expression pattern between hypothalami of the ESR females compared to their 60-day-old controls. EB-dependent expression of these genes was further analyzed by low-density cDNA array using cDNA probe sets reverse-transcribed from the same groups; 98 genes were confirmed to be differentially expressed. We selected 41 clones that showed higher density differences between the two probe sets than mean density difference in control cyclophilin cDNA blots in the cDNA array. After being cloned into pGEM-T vectors, their sequences were analyzed. Homology searches identified four genes as a protein kinase C (PKC)-binding protein, NELL2 (clone 6-1), a thyroid nuclear factor, TTF-1 (9-1), Munc18-1 (17-6), and leuserpin-2 (18-5). The other 22 genes were similar to reported genes or cDNAs such as mouse kinesin-associated protein 3 (KAP3, 8b), mouse IgE binding lectin (15-1), normalized rat brain cDNA (5-1), rat cDNA (8-1) and rat embryonic cDNA (17-1). Fifteen clones such as clone 7-3 showed no match in the GenBank Database. Further characterization of eight clones (17-1, 7-3, 8-1, 5-1, NELL2, KAP3 homolog, IgE binding lectin homolog, and TTF-1) showed that their expression in the adult female rat hypothalamus is sensitive to neonatal treatment with EB. They showed brain-specific expression and moreover, showed an increase in their mRNA level before the initiation of puberty. Some of them showed gender differences in their different postnatal expression pattern. We speculate that further study will demonstrate that many of the E-regulated genes identified in the present study play important roles in the regulation of the sexual differentiation and E-dependent maturation of the hypothalamus." @default.
• W2023006665 created "2016-06-24" @default.
• W2023006665 creator A5026999837 @default.
• W2023006665 creator A5035243568 @default.
• W2023006665 creator A5036204847 @default.
• W2023006665 creator A5036823265 @default.
• W2023006665 creator A5049737479 @default.
• W2023006665 creator A5070586345 @default.
• W2023006665 creator A5077313683 @default.
• W2023006665 creator A5091214693 @default.
• W2023006665 date "2001-12-01" @default.
• W2023006665 modified "2023-10-01" @default.
• W2023006665 title "Low-density cDNA array-coupled to PCR differential display identifies new estrogen-responsive genes during the postnatal differentiation of the rat hypothalamus" @default.
• W2023006665 cites W1496124518 @default.
• W2023006665 cites W1497664421 @default.
• W2023006665 cites W1819608959 @default.
• W2023006665 cites W1844026860 @default.
• W2023006665 cites W1895711312 @default.
• W2023006665 cites W1963842482 @default.
• W2023006665 cites W1970725106 @default.
• W2023006665 cites W1977707793 @default.
• W2023006665 cites W1978368163 @default.
• W2023006665 cites W1978736710 @default.
• W2023006665 cites W1984643713 @default.
• W2023006665 cites W1986951502 @default.
• W2023006665 cites W1988502218 @default.
• W2023006665 cites W1989155678 @default.
• W2023006665 cites W2000467114 @default.
• W2023006665 cites W2012630820 @default.
• W2023006665 cites W2017115998 @default.
• W2023006665 cites W2018464179 @default.
• W2023006665 cites W2025034597 @default.
• W2023006665 cites W2036111836 @default.
• W2023006665 cites W2037350042 @default.
• W2023006665 cites W2042490889 @default.
• W2023006665 cites W2049442493 @default.
• W2023006665 cites W2052268500 @default.
• W2023006665 cites W2054357633 @default.
• W2023006665 cites W2060999889 @default.
• W2023006665 cites W2066441904 @default.
• W2023006665 cites W2069012385 @default.
• W2023006665 cites W2071693558 @default.
• W2023006665 cites W2082631326 @default.
• W2023006665 cites W2085124463 @default.
• W2023006665 cites W2085887859 @default.
• W2023006665 cites W2088494357 @default.
• W2023006665 cites W2091723964 @default.
• W2023006665 cites W2093963412 @default.
• W2023006665 cites W2098357756 @default.
• W2023006665 cites W2104699052 @default.
• W2023006665 cites W2106697461 @default.
• W2023006665 cites W2118770662 @default.
• W2023006665 cites W2129738259 @default.
• W2023006665 cites W2132411277 @default.
• W2023006665 cites W2143020022 @default.
• W2023006665 cites W2164474217 @default.
• W2023006665 cites W2188426128 @default.
• W2023006665 cites W2224929038 @default.
• W2023006665 cites W4294216491 @default.
• W2023006665 cites W76371562 @default.
• W2023006665 doi "https://doi.org/10.1016/s0169-328x(01)00302-3" @default.
• W2023006665 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11750068" @default.
• W2023006665 hasPublicationYear "2001" @default.
• W2023006665 type Work @default.
• W2023006665 sameAs 2023006665 @default.
• W2023006665 citedByCount "18" @default.
• W2023006665 countsByYear W20230066652017 @default.
• W2023006665 countsByYear W20230066652018 @default.
• W2023006665 countsByYear W20230066652022 @default.
• W2023006665 crossrefType "journal-article" @default.
• W2023006665 hasAuthorship W2023006665A5026999837 @default.
• W2023006665 hasAuthorship W2023006665A5035243568 @default.
• W2023006665 hasAuthorship W2023006665A5036204847 @default.
• W2023006665 hasAuthorship W2023006665A5036823265 @default.
• W2023006665 hasAuthorship W2023006665A5049737479 @default.
• W2023006665 hasAuthorship W2023006665A5070586345 @default.
• W2023006665 hasAuthorship W2023006665A5077313683 @default.
• W2023006665 hasAuthorship W2023006665A5091214693 @default.
• W2023006665 hasConcept C104317684 @default.
• W2023006665 hasConcept C105580179 @default.
• W2023006665 hasConcept C150194340 @default.
• W2023006665 hasConcept C153911025 @default.
• W2023006665 hasConcept C187882448 @default.
• W2023006665 hasConcept C2776657240 @default.
• W2023006665 hasConcept C54355233 @default.
• W2023006665 hasConcept C86803240 @default.
• W2023006665 hasConceptScore W2023006665C104317684 @default.
• W2023006665 hasConceptScore W2023006665C105580179 @default.
• W2023006665 hasConceptScore W2023006665C150194340 @default.
• W2023006665 hasConceptScore W2023006665C153911025 @default.
• W2023006665 hasConceptScore W2023006665C187882448 @default.
• W2023006665 hasConceptScore W2023006665C2776657240 @default.
• W2023006665 hasConceptScore W2023006665C54355233 @default.
• W2023006665 hasConceptScore W2023006665C86803240 @default.
• W2023006665 hasIssue "2" @default.
• W2023006665 hasLocation W20230066651 @default.
• W2023006665 hasLocation W20230066652 @default.
• W2023006665 hasOpenAccess W2023006665 @default.

• next