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- W2023008233 abstract "Secretion of the heterologous Kluyveromyces lactis β-galactosidase into culture medium by several Saccharomyces cerevisiae osmotic-remedial thermosensitive-autolytic mutants was assayed and proved that new metabolic abilities were conferred since the constructed strains were able to grow in lactose-containing media. Cell growth became independent of a lactose-uptake mechanism. Higher levels of extra-cellular and intra-cellular β-galactosidase production, lactose consumption and growth were obtained with the LHDP1 strain, showing a thermosensitive-autolytic phenotype as well as being peptidase-defective. The recombinant strain LHDP1 presented the highest β-galactosidase yields from biomass and the lowest ethanol levels from lactose. This strain is effective for the heterologous production and release of K. lactis β-galactosidase into the extra-cellular medium after osmotic shock." @default.
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- W2023008233 date "2004-04-08" @default.
- W2023008233 modified "2023-10-18" @default.
- W2023008233 title "Engineered autolytic yeast strains secreting Kluyveromyces lactis β-galactosidase for production of heterologous proteins in lactose media" @default.
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- W2023008233 doi "https://doi.org/10.1016/j.jbiotec.2003.10.030" @default.
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