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• W2023015152 abstract "In this paper we investigate the size, structure and scaling relationships in glycogen isolated from five different animal sources. For this purpose a versatile fractionation technique, asymmetrical flow field-flow fractionation (AsFlFFF), coupled to multi-angle light scattering, is utilized. For determination of the average degree of branching 1H NMR is utilized. The results give a detailed insight into the physico-chemical properties of glycogen over the whole size distribution. The results show that glycogen is a hyper branched macromolecule with wide size distributions, and in some samples two major populations are clearly observed which most likely correspond to β- and α-particles of glycogen. The results also illustrates that glycogen is a polysaccharide showing rather diverse conformational properties, over the size distribution, depending on its origin and the extraction procedure. The ratio between root-mean-square radius and hydrodynamic radius varies depending of both sample origin the molar mass of the macromolecules, reflecting differences in conformation and scaling within the size distribution. Thus, a priori assumptions regarding the rrms/rh are difficult to make and rrms/rh based on average properties give an incomplete description of the properties. Furthermore, the results display the strength of the apparent density (as obtained from AsFlFFF-MALS-RI) as a characterization parameter for scaling in disperse macromolecules." @default.
• W2023015152 created "2016-06-24" @default.
• W2023015152 creator A5048132031 @default.
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• W2023015152 date "2011-11-01" @default.
• W2023015152 modified "2023-09-29" @default.
• W2023015152 title "Size, structure and scaling relationships in glycogen from various sources investigated with asymmetrical flow field-flow fractionation and 1H NMR" @default.
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• W2023015152 doi "https://doi.org/10.1016/j.ijbiomac.2011.05.016" @default.
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